| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_709b4a26ef028713f9b302509eb824d7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3690d91c97567ab1af2ceb95bd9c470a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff47a6c30c863b4e7334d82ba857d6b2 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2531-125 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1096
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2001-02-09^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56a36a25cc464fd0cb776abd1fba5672
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f157044323e51a8f15699c552190f5e6 |
| publicationDate |
2002-10-17^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-0159101-A9 |
| titleOfInvention |
Method for amplifying full length single strand polynucleotide sequences |
| abstract |
Applicants have identified a novel amplification method that uses two specific primers to clone both the 5' and 3' cDNA ends in a single reaction. This new method also uses the first strand of cDNA obtained after reverse transcription of mRNA rather than double stranded cDNA, further increasing efficiency of amplification. According to the invention the first strand of cDNA is self-ligated using a DNA ligase. |
| priorityDate |
2000-02-10^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |