| abstract |
A method to improve neural cell viability in brain or spinal cord tissue after brain or spinal cord injury or surgery is provided. This method comprises applying a sterile liquid medium to the brain or spinal cord tissue, wherein the sterile aqueous liquid medium comprises 0 to about 3000 µM CaCI2, about 0.1 to about 1.2 µM Fe(NO3)3, about 2500 to about 10000 µM KCI, 0 to about 4000 µM MgCI2, about 30000 to about 150000 µM NaCI, about 100 to about 30000 µM NaHCO3, about 250 to about 4000 µM NaH2PO4, about 0.01 to about 0.4 µM sodium selenite, about 0.2 to about 2 µM ZnSO4, about 2500 to about 50000 µM D-glucose, about 1 to about 50 µM L-carnitine, about 3 to about 80 µM ethanolamine, about 15 to about 400 µM D(+)-galactose, about 40 to about 800 µM putrescine, about 20 to about 500 µM sodium pyruvate, and growth-promoting essential fatty acids, hormones, amino acids, vitamins and anti-oxidants in amounts effective for neuron growth, and wherein the medium is essentially free of ferrous sulfate, glutamate, and aspartate. |