| abstract |
The present invention provides a recombinant polynucleotide, the polynucleotide comprising a first and a second sequence, the first sequence encoding a signal peptide comprising a TAT signal and a Sec avoidance signal and the second sequence encoding a heterologous protein. The sequence of the signal peptide is M - X1 - K/R - X2 - K/R - X3 - RR - X4 - K/R - A in which X1 is a sequence of 0 to 10 amino acids, X2 is a sequence of 0 to 3 amino acids, X3 is a sequence of 0 to 10 amino acids and X4 is a sequence of 15 to 24 amino acids in which at least 75 % up to about 90 % of the residues are hydrophobic. |