| abstract |
The present teachings relate to methods, compositions, and kids for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identify ans quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiements of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiements of the present teachings provide for highly multiplexed detection. identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures. |