| abstract |
It is intended to provide genes and proteins which can impart boron-tolerance to organisms and, therefore, are expected as usable in constructing plants tolerant to boron excess. Several genes of a higher plant Arabidopsis are expressed in a yeast, which is a model organism of eucaryotes, to thereby find five types of genes capable of imparting boron-tolerance to the yeast, i.e., AtPAB2, AtRBP47c’, AtRPS20B, AtMYB13 and AtMYB68, AtRBP45a, AtRBP45b, AtRBP45c, AtRBP45d, AtRBP47a, AtRBP47b, AtRBP47c, AtUBP1a, AtUBP1b and AtUBP1c. It is also found out that the key factor of the boron toxicity mechanism resides in the specific inhibition of splicing and thus a gene relating to the acceleration of the splicing efficiency is a gene capable of imparting boron-tolerance. |