| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4047b0f33ae605e46e352cfbf9da7588
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d654431b598534fc1609e36c31825981
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_764094c9612c9261f162d0f095108a11 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-16 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6893
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-86
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07H23-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-115 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H19-04
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07H21-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K48-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 |
| filingDate |
2008-07-17^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_217e06acb4c087aebf4c2452fd9aa83f
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c10f4daa2a9631f237164305ffa7429 |
| publicationDate |
2009-01-22^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
WO-2009012363-A2 |
| titleOfInvention |
Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof |
| abstract |
The present disclosure encompasses oligonucleotide aptamers selectively binding a target glycosylated polypeptide or protein, and having biased affinity for the glycan through a boronic acid linked to a nucleosidic base of a nucleotide(s). The disclosure further encompasses methods for isolating an aptamer(s) selectively binding a target glycosylated polypeptide, where, from a population of randomized oligonucleotides that have at least one nucleotide having a boronic acid label linked to a base, is selected a first subpopulation of aptamers binding to the target glycosylated polypeptide or protein. This subpopulation is then amplified without using boronic acid-modified TTP, and amplification products not binding to a target glycosylated polypeptide or protein are selected. The second subpopulation of aptamers is then amplified using boronic acid-modified TTP to provide a population of boronic acid-modified aptamers capable of selectively binding to a glycosylation site of a target polypeptide or protein. Other aspects of the disclosure encompass methods for the use of the modified aptamers to detect glycosylated species of a polypeptide or protein. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2310054-A2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-2011144187-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-10450673-B2
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/EP-2310054-A4
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2017048147-A1 |
| priorityDate |
2007-07-19^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |