| abstract |
Provided is a method for producing pancreatic blast cells, the method comprising a step for culturing PDX1-positive, NKX6.1-negative cells in a medium containing KGF, EGF, and BMP inhibitor. The culture step can be performed by suspension culture or by adhesion culture. Culture by adhesion culture can be performed in a medium further comprising ROCK inhibitor or non-muscle myosin II inhibitor. |