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publicationNumber WO-2016142550-A1
titleOfInvention A process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids
abstract It is disclosed a process for producing a hydrolyzed mixture from a pre-treated ligno-cellulosic slurry comprising a slurry liquid and slurry solids. In the disclosed process, at least a portion of a cellulase from an enzyme cocktail having an initial cellulase activity is adsorbed onto the slurry solids of the pre-treated ligno-cellulosic biomass slurry to create an adsorbed cellulase, wherein the adsorbed cellulase has an adsorbed cellulase activity which is greater than 45% of the initial cellulase activity. The pre-treated ligno-cellulosic slurry with adsorbed enzymes is subjected to enzymatic hydrolysis to produce the hydrolyzed mixture. The enzymatic hydrolysis may comprise more than one hydrolysis step, which may be continuous or batch.
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