| abstract |
The present invention provides an expression system and process for the production of diphtheria toxin polypeptides or mutated forms thereof, such as the CRM197 toxoid polypeptide, in genetically modified E. coli , with high yield. . The system and process are based on the decoupling of biomass growth from recombinant protein induction, i.e. using a protein production inducer that can not be used as a carbon source for growth by bacteria. <i /> The invention also relates to the use of specific components and states that enhance protein yields. |