| abstract |
The present invention relates to a primer set comprising a primer represented by formula 1 below.n[Formula 1] 5'-X-Y-Z-3',nIn the formula, X is a first binding region comprising a 15 to 25 bp hybridizing nucleotide sequence complementary to a template nucleic acid to be hybridized, Y is a 3 to 10 bp ring forming region, and Z is a second binding region comprising a 5 to 10 bp hybridizing nucleotide sequence complementary to the template nucleic acid, and nucleotides corresponding to an allele and 1 to 3 bp non-complementary nucleotides continuously binding thereto, wherein X, Y and Z are deoxyribonucleotides or ribonucleotides. |