| abstract |
The present disclosure relates to rotatable platform for use in Lab-on-disc (LoD) applications, and LoD microfluidic device having the rotatable platform and which is suitable for analysis of a fluid sample. The present disclosure further relates to realtime monitoring of cells using a centrifugal microfluidic platform. In particular, the present disclosure relates to a mobile LoD device, which can be used in remote destinations and for point of care applications. One embodiment relates to a method for monitoring microorganisms under the constant supply of nutrients, comprising the step of inoculating the cells in a culture chamber in a rotatable microfluidic platform, rotating the platform such that liquid in a reservoir connected to or located on the platform, the liquid comprising nutrients for the cells, is constantly supplied to the cells in the culture chamber by means of shear / centrifugal force resulting from rotating the platform, and continuously monitoring the cells during rotation of the platform by means of imaging, electrochemical and/or electrical measurements. |