| abstract |
Disclosed is a method for preparing a bifunctional hybrid film for tumor exosome self-calibration detection. A bifunctional hybrid film, namely, aptamer-BPNSs/Fc/ZIF-67/ITO, is constructed on the basis of simple self-assembly of black phosphorus nanosheets BPNSs, an aptamer and a ferrocene Fc-doped cobalt-based metal organic framework ZIF-67 complex on an indium tin oxide ITO electrode. The aptamer, which is marked by methylene blue MB, specifically binds to a CD63 protein to accurately capture protein. The protein is a specific biomolecule carried by a breast cancer MCF-7 cell exosome, and realizes detection of the tumor cell exosome. MB is used as a response signal, Fc is used as a reference, and a self-calibration sensor for quantitative detection of the tumor exosome is constructed. Compared with the prior art, the method is convenient to operate, highly sensitive, low in terms of cost, has a high specificity, can be used as a new exosome self-calibration detection method, and is used for the quantitative detection of exosomes in biomedical samples. |