File:Direct-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Direct-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 1 min 10 s, 352 × 288 pixels, 260 kbps overall, file size: 2.17 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary

[edit]
Description
English: Rotating three-dimensional reconstructions of LGAC with and without nocodazole treatment, at different phases of CCH stimulation. LGAC expressing YFP-Rab27b are shown, comparing the effects of no treatment to nocodazole treatment on the subapical SV pool within representative acini by acquisition of z-stacks images under different conditions. Control and treated acini were imaged at resting phase prior to any stimulation, imaged after 15 m stimulation with 100 µM CCH, and then also imaged after 15 m CCH stimulation followed by multiple washes with fresh media, and 60 m recovery time without or with additional nocodazole. Results are typical of those from N = 5 separate preparations. Bar represents 5 µm.
Date
Source Video S1 from Chiang L, Karvar S, Hamm-Alvarez S (2012). "Direct Imaging of RAB27B-Enriched Secretory Vesicle Biogenesis in Lacrimal Acinar Cells Reveals Origins on a Nascent Vesicle Budding Site". PLOS ONE. DOI:10.1371/journal.pone.0031789. PMID 22363735. PMC: 3282733.
Author Chiang L, Karvar S, Hamm-Alvarez S
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current05:35, 15 November 20121 min 10 s, 352 × 288 (2.17 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 240P 127 kbps Completed 11:30, 24 August 2018 46 s
WebM 360P 578 kbps Completed 04:51, 30 November 2023 7.0 s
QuickTime 144p (MJPEG) 1.04 Mbps Completed 13:47, 22 October 2024 3.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Direct-Imaging-of-RAB27B-Enriched-Secretory-Vesicle-Biogenesis-in-Lacrimal-Acinar-Cells-Reveals-pone.0031789.s001.ogv&oldid=496979085"