Modulation of Syndecan-1 Shedding after Hemorrhagic Shock and Resuscitation
Figure 3
Endothelial cell permeability is decreased by FFP in an in vitro model of endothelial injury.
HUVEC's were stimulated with IL-1β and subjected to hypoxia for four hours followed by rexoygenation and incubation for an additional four hours in media alone (hypoxia/reoxygenation, HR), 5% lactated Ringers (LR), or 5% FFP, and compared to normoxia. In vitro permeability was assessed by fluoresceine-isothiocyanate [FITC] labeled dextran transport. Permeability was significantly increased by HR compared to normoxia, lessened by LR, but FFP decreased permeability to below that of normoxia. Results are presented as means ± SEM, n = 6/group. Means notated with letters indicate statistical differences between groups.
