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Plaque formation and isolation of pure lines with poliomyelitis viruses.

The Journal of Experimental Medicine, 01 Feb 1954, 99(2):167-182
https://doi.org/10.1084/jem.99.2.167 PMID: 13130792 PMCID: PMC2180341

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Abstract 

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 10(8) plaque-forming particles per ml., were obtained.

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J Exp Med. 1954 Jan 31; 99(2): 167–182.
PMCID: PMC2180341
PMID: 13130792

PLAQUE FORMATION AND ISOLATION OF PURE LINES WITH POLIOMYELITIS VIRUSES

R. Dulbecco and Marguerite Vogt
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Abstract

Plaques have been produced with the three types of poliomyelitis viruses on monolayer tissue cultures of monkey kidney and monkey testis. The number of plaques was proportional to the concentration of the virus. Each plaque originates, therefore, from a single virus particle, defined as the virus unit that is unseparable by dilution. The plaques are due to the specific action of the virus since they are suppressed by type-specific antiserum. Pure virus lines were established by isolating the virus population produced in single plaques. These derived virus lines had the same morphological, serological, and pathogenic properties as the parent strain. High titer virus stocks, with titers up to 7 x 108 plaque-forming particles per ml., were obtained.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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