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Density Centrifugation Method for Recovering Rhizobium spp. from Soil for Fluorescent-Antibody Studies.

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  • 1. Departments of Soil Science and Agronomy, North Carolina State University, Raleigh, North Carolina 27650.
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    • Wollum AG 1
    (1 author)

Applied and Environmental Microbiology, 01 Feb 1980, 39(2):466-469
https://doi.org/10.1128/aem.39.2.466-469.1980 PMID: 16345522 PMCID: PMC291356

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Abstract 

A density centrifugation procedure has been developed as a replacement for soil flocculation and clarification steps employed in quantitative fluorescent-antibody studies on Rhizobium in soils. Near-quantitative recovery of added cells of two strains of Rhizobium japonicum and two strains of R. phaseoli was achieved from six soils with various properties. It is proposed that this technique may prove useful in separating other soil microorganisms from soil particles in ecological studies employing fluorescent-antibody techniques.

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Appl Environ Microbiol. 1980 Feb; 39(2): 466–469.
PMCID: PMC291356
PMID: 16345522

Density Centrifugation Method for Recovering Rhizobium spp. from Soil for Fluorescent-Antibody Studies †

A. G. Wollum, II1 and R. H. Miller2
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Abstract

A density centrifugation procedure has been developed as a replacement for soil flocculation and clarification steps employed in quantitative fluorescent-antibody studies on Rhizobium in soils. Near-quantitative recovery of added cells of two strains of Rhizobium japonicum and two strains of R. phaseoli was achieved from six soils with various properties. It is proposed that this technique may prove useful in separating other soil microorganisms from soil particles in ecological studies employing fluorescent-antibody techniques.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Bohlool BB, Brock TD. Immunofluorescence approach to the study of the ecology of Thermoplasma acidophilum in coal refuse material. Appl Microbiol. 1974 Jul;28(1):11–16. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cole MA, Elkan GH. Transmissible resistance to penicillin G, neomycin, and chloramphenicol in Rhizobium japonicum. Antimicrob Agents Chemother. 1973 Sep;4(3):248–253. [Europe PMC free article] [Abstract] [Google Scholar]
  • Fliermans CB, Schmidt EL. Autoradiography and immunofluorescence combined for autecological study of single cell activity with Nitrobacter as a model system. Appl Microbiol. 1975 Oct;30(4):676–684. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hobbie JE, Daley RJ, Jasper S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl Environ Microbiol. 1977 May;33(5):1225–1228. [Europe PMC free article] [Abstract] [Google Scholar]
  • Reyes VG, Schmidt EL. Population Densities of Rhizobium japonicum Strain 123 Estimated Directly in Soil and Rhizospheres. Appl Environ Microbiol. 1979 May;37(5):854–858. [Europe PMC free article] [Abstract] [Google Scholar]
  • Strayer RF, Tiedje JM. Application of the fluorescent-antibody technique to the study of a methanogenic bacterium in lake sediments. Appl Environ Microbiol. 1978 Jan;35(1):192–198. [Europe PMC free article] [Abstract] [Google Scholar]
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