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Evidence that miRNAs are different from other RNAs.

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  • 1. The Institute of Environmental and Human Health (TIEHH), and Department of Environmental Toxicology, Texas Tech University, Lubbock, Texas 79409-1163, USA.
      Authors
    • Zhang BH 1
    (1 author)

ORCIDs linked to this article

Cellular and Molecular Life Sciences : CMLS, 01 Jan 2006, 63(2):246-254
https://doi.org/10.1007/s00018-005-5467-7 PMID: 16395542 PMCID: PMC11136112

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Abstract 

An examination of 513 known pre-miRNAs and 237 other RNAs (tRNA, rRNA, and mRNA) revealed that miRNAs were significantly different from other RNAs (p < 0.001). miRNA genes were less conserved than other RNA genes, although their mature miRNA sequences were highly conserved. The A+U content of pre-miRNAs was higher than non-coding RNA (p < 0.001), but lower than mRNAs. The nucleotides in pre-miRNAs formed more hydrogen bonds and base pairs than in other RNAs. miRNAs had higher negative adjusted minimal folding free energies than other RNAs except tRNAs (p < 0.001). The MFE index (MFEI) was a sufficient criterion to distinguish miRNAs from all coding and non-coding RNAs (p < 0.001). The MFEI for miRNAs was 0.97, significantly higher than tRNAs (0.64), rRNAs (0.59), or mRNAs (0.65). Our findings should facilitate the prediction and identification of new miRNAs using computational and experimental strategies.

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Cell Mol Life Sci. 2006 Jan; 63(2): 246–254.
Published online 2006 Jan 6. https://doi.org/10.1007/s00018-005-5467-7
PMCID: PMC11136112
PMID: 16395542

Evidence that miRNAs are different from other RNAs

B. H. Zhang, X. P. Pan, S. B. Cox, G. P. Cobb, and T. A. Andersoncorresponding author
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Abstract.

An examination of 513 known pre-miRNAs and 237 other RNAs (tRNA, rRNA, and mRNA) revealed that miRNAs were significantly different from other RNAs (p < 0.001). miRNA genes were less conserved than other RNA genes, although their mature miRNA sequences were highly conserved. The A+U content of pre-miRNAs was higher than non-coding RNA (p < 0.001), but lower than mRNAs. The nucleotides in pre-miRNAs formed more hydrogen bonds and base pairs than in other RNAs. miRNAs had higher negative adjusted minimal folding free energies than other RNAs except tRNAs (p < 0.001). The MFE index (MFEI) was a sufficient criterion to distinguish miRNAs from all coding and non-coding RNAs (p < 0.001). The MFEI for miRNAs was 0.97, significantly higher than tRNAs (0.64), rRNAs (0.59), or mRNAs (0.65). Our findings should facilitate the prediction and identification of new miRNAs using computational and experimental strategies.

Key words. MicroRNA, plant, tRNA, rRNA, mRNA, bioinformatics, minimal folding free energy index, MFEI

Footnotes

Received 5 October 2005; received after revision 4 November 2005; accepted 16 November 2005

Articles from Cellular and Molecular Life Sciences: CMLS are provided here courtesy of Springer

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