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Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity.

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Affiliations

  • 1. Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium.
      Authors
    • Zambryski P 1
    (1 author)

The EMBO Journal, 01 Jan 1983, 2(12):2143-2150
https://doi.org/10.1002/j.1460-2075.1983.tb01715.x PMID: 16453482 PMCID: PMC555426

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Abstract 

A Ti plasmid mutant was constructed in which all the on-cogenic functions of the T-DNA have been deleted and replaced by pBR322. This Ti plasmid, pGV3850, still mediates efficient transfer and stabilization of its truncated T-DNA into infected plant cells. Moreover, integration and expression of this minimal T-DNA in plant cells does not interfere with normal plant cell differentiation. A DNA fragment cloned in a pBR vector can be inserted in the pGV3850 T-region upon a single recombination event through the pBR322 region of pGV3850 producing a co-integrate useful for the transformation of plant cells. Based upon these properties, pGV3850 is proposed as an extremely versatile vector for the introduction of any DNA of interest into plant cells.

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EMBO J. 1983; 2(12): 2143–2150.
PMCID: PMC555426
PMID: 16453482

Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity

P. Zambryski,1 H. Joos,1 C. Genetello,1 J. Leemans,24 M. Van Montagu,12* and J. Schell13*
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Abstract

A Ti plasmid mutant was constructed in which all the on-cogenic functions of the T-DNA have been deleted and replaced by pBR322. This Ti plasmid, pGV3850, still mediates efficient transfer and stabilization of its truncated T-DNA into infected plant cells. Moreover, integration and expression of this minimal T-DNA in plant cells does not interfere with normal plant cell differentiation. A DNA fragment cloned in a pBR vector can be inserted in the pGV3850 T-region upon a single recombination event through the pBR322 region of pGV3850 producing a co-integrate useful for the transformation of plant cells. Based upon these properties, pGV3850 is proposed as an extremely versatile vector for the introduction of any DNA of interest into plant cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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