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Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.

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  • 1. Department of Chemistry, Rutgers University, New Brunswick, NJ 08855.
      Authors
    • Zhou YH 1
    (1 author)

Nucleic Acids Research, 01 Nov 1991, 19(21):6052
https://doi.org/10.1093/nar/19.21.6052 PMID: 1658751 PMCID: PMC329070

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Nucleic Acids Res. 1991 Nov 11; 19(21): 6052.
PMCID: PMC329070
PMID: 1658751

Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.

Y H Zhou, X P Zhang, and R H Ebright
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Selected References

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  • Tindall KR, Kunkel TA. Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Biochemistry. 1988 Aug 9;27(16):6008–6013. [Abstract] [Google Scholar]
  • Eckert KA, Kunkel TA. High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleic Acids Res. 1990 Jul 11;18(13):3739–3744. [Europe PMC free article] [Abstract] [Google Scholar]
  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. [Abstract] [Google Scholar]
  • Keohavong P, Thilly WG. Fidelity of DNA polymerases in DNA amplification. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9253–9257. [Europe PMC free article] [Abstract] [Google Scholar]
  • Eckert KA, Kunkel TA. DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl. 1991 Aug;1(1):17–24. [Abstract] [Google Scholar]
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Funding 

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NIGMS NIH HHS (1)