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Organization of the micronuclear genome of oxytricha nova.

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  • 1. Laboratory for Cell, Molecular and Developmental Biology, Department of Biological Sciences, University of Illinois, Chicago, Illinois 60680.
      Authors
    • Jahn CL 1
    (1 author)

Genetics, 01 Sep 1988, 120(1):123-134
https://doi.org/10.1093/genetics/120.1.123 PMID: 17246474 PMCID: PMC1203484

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Abstract 

In the hypotrichous ciliated protozoan Oxytricha nova, approximately 95% of the micronuclear genome, including all of the repetitive DNA and most of the unique sequence DNA, is eliminated during the formation of the macronuclear genome. We have examined the interspersion patterns of repetitive and unique and eliminated and retained sequences in the micronuclear genome by characterizing randomly selected clones of micronuclear DNA. Three major classes of clones have been defined: (1) those containing primarily unique, retained sequences; (2) those containing only unique, eliminated sequences; and (3) those containing only repetitive, eliminated sequences. Clones of type one and three document two aspects of organization observed previously: clustering of macronuclear destined sequences and the presence of a prevalent repetitive element. Clones of the second type demonstrate for the first time that eliminated unique sequence DNA occurs in long stretches uninterrupted by repetitive sequences. To further examine repetitive sequence interspersion, we characterized the repetitive sequence family that is present in 50% of the clones (class three above). A consensus map of this element was obtained by mapping approximately 80 phage clones and by hybridization to digests of micronuclear DNA. The repeat element is extremely large (approximately 24 kb) and is interspersed with both macronuclear destined sequences and eliminated unique sequences.

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Logo of geneticsLink to Publisher's site
Genetics. 1988 Sep; 120(1): 123–134.
PMCID: PMC1203484
PMID: 17246474

Organization of the Micronuclear Genome of Oxytricha Nova

C. L. Jahn, K. E. Prescott, and M. W. Waggener
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Abstract

In the hypotrichous ciliated protozoan Oxytricha nova, approximately 95% of the micronuclear genome, including all of the repetitive DNA and most of the unique sequence DNA, is eliminated during the formation of the macronuclear genome. We have examined the interspersion patterns of repetitive and unique and eliminated and retained sequences in the micronuclear genome by characterizing randomly selected clones of micronuclear DNA. Three major classes of clones have been defined: (1) those containing primarily unique, retained sequences; (2) those containing only unique, eliminated sequences; and (3) those containing only repetitive, eliminated sequences. Clones of type one and three document two aspects of organization observed previously: clustering of macronuclear destined sequences and the presence of a prevalent repetitive element. Clones of the second type demonstrate for the first time that eliminated unique sequence DNA occurs in long stretches uninterrupted by repetitive sequences. To further examine repetitive sequence interspersion, we characterized the repetitive sequence family that is present in 50% of the clones (class three above). A consensus map of this element was obtained by mapping approximately 80 phage clones and by hybridization to digests of micronuclear DNA. The repeat element is extremely large (approximately 24 kb) and is interspersed with both macronuclear destined sequences and eliminated unique sequences.

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Selected References

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  • Altschuler MI, Yao MC. Macronuclear DNA of Tetrahymena thermophila exists as defined subchromosomal-sized molecules. Nucleic Acids Res. 1985 Aug 26;13(16):5817–5831. [Europe PMC free article] [Abstract] [Google Scholar]
  • Austerberry CF, Yao MC. Nucleotide sequence structure and consistency of a developmentally regulated DNA deletion in Tetrahymena thermophila. Mol Cell Biol. 1987 Jan;7(1):435–443. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cartinhour SW, Herrick GA. Three different macronuclear DNAs in Oxytricha fallax share a common sequence block. Mol Cell Biol. 1984 May;4(5):931–938. [Europe PMC free article] [Abstract] [Google Scholar]
  • Conover RK, Brunk CF. Macronuclear DNA molecules of Tetrahymena thermophila. Mol Cell Biol. 1986 Mar;6(3):900–905. [Europe PMC free article] [Abstract] [Google Scholar]
  • Dawson D, Herrick G. Telomeric properties of C4A4-homologous sequences in micronuclear DNA of Oxytricha fallax. Cell. 1984 Jan;36(1):171–177. [Abstract] [Google Scholar]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [Abstract] [Google Scholar]
  • Frischauf AM, Lehrach H, Poustka A, Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. [Abstract] [Google Scholar]
  • Helftenbein E. Nucleotide sequence of a macronuclear DNA molecule coding for alpha-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon. Nucleic Acids Res. 1985 Jan 25;13(2):415–433. [Europe PMC free article] [Abstract] [Google Scholar]
  • Herrick G, Cartinhour S, Dawson D, Ang D, Sheets R, Lee A, Williams K. Mobile elements bounded by C4A4 telomeric repeats in Oxytricha fallax. Cell. 1985 Dec;43(3 Pt 2):759–768. [Abstract] [Google Scholar]
  • Herrick G, Hunter D, Williams K, Kotter K. Alternative processing during development of a macronuclear chromosome family in Oxytricha fallax. Genes Dev. 1987 Dec;1(10):1047–1058. [Abstract] [Google Scholar]
  • Howard EA, Blackburn EH. Reproducible and variable genomic rearrangements occur in the developing somatic nucleus of the ciliate Tetrahymena thermophila. Mol Cell Biol. 1985 Aug;5(8):2039–2050. [Europe PMC free article] [Abstract] [Google Scholar]
  • Karrer KM. Germ line-specific DNA sequences are present on all five micronuclear chromosomes in Tetrahymena thermophila. Mol Cell Biol. 1983 Nov;3(11):1909–1919. [Europe PMC free article] [Abstract] [Google Scholar]
  • Klobutcher LA, Vailonis-Walsh AM, Cahill K, Ribas-Aparicio RM. Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova. Mol Cell Biol. 1986 Nov;6(11):3606–3613. [Europe PMC free article] [Abstract] [Google Scholar]
  • Lauth MR, Spear BB, Heumann J, Prescott DM. DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha. Cell. 1976 Jan;7(1):67–74. [Abstract] [Google Scholar]
  • Moritz KB, Roth GE. Complexity of germline and somatic DNA in Ascaris. Nature. 1976 Jan 1;259(5538):55–57. [Abstract] [Google Scholar]
  • Orgel LE, Crick FH. Selfish DNA: the ultimate parasite. Nature. 1980 Apr 17;284(5757):604–607. [Abstract] [Google Scholar]
  • Prescott DM, Murti KG. Chromosome structure in ciliated protozoans. Cold Spring Harb Symp Quant Biol. 1974;38:609–618. [Abstract] [Google Scholar]
  • Pruitt RE, Meyerowitz EM. Characterization of the genome of Arabidopsis thaliana. J Mol Biol. 1986 Jan 20;187(2):169–183. [Abstract] [Google Scholar]
  • Ribas-Aparicio RM, Sparkowski JJ, Proulx AE, Mitchell JD, Klobutcher LA. Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA. Genes Dev. 1987 Jun;1(4):323–336. [Abstract] [Google Scholar]
  • Swanton MT, Greslin AF, Prescott DM. Arrangement of coding and non-coding sequences in the DNA molecules coding for rRNAs in Oxytricha sp. DNA of ciliated protozoa. VII. Chromosoma. 1980;77(2):203–215. [Abstract] [Google Scholar]
  • Swanton MT, Heumann JM, Prescott DM. Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII. Chromosoma. 1980;77(2):217–227. [Abstract] [Google Scholar]
  • Yao MC, Gorovsky MA. Comparison of the sequences of macro- and micronuclear DNA of Tetrahymena pyriformis. Chromosoma. 1974;48(1):1–18. [Abstract] [Google Scholar]
  • Yao MC, Choi J, Yokoyama S, Austerberry CF, Yao CH. DNA elimination in Tetrahymena: a developmental process involving extensive breakage and rejoining of DNA at defined sites. Cell. 1984 Feb;36(2):433–440. [Abstract] [Google Scholar]
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