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Combination of external beam radiotherapy (EBRT) with intratumoral injection of dendritic cells as neo-adjuvant treatment of high-risk soft tissue sarcoma patients.

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  • 1. H. Lee Moffitt Cancer Center, Tampa, FL 33612, USA.
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    • Finkelstein SE 1
    (1 author)

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International Journal of Radiation Oncology, Biology, Physics, 11 Mar 2011, 82(2):924-932
https://doi.org/10.1016/j.ijrobp.2010.12.068 PMID: 21398051 PMCID: PMC4241354

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Abstract 

Purpose

The goal of this study was to determine the effect of combination of intratumoral administration of dendritic cells (DC) and fractionated external beam radiation (EBRT) on tumor-specific immune responses in patients with soft-tissue sarcoma (STS).

Methods and material

Seventeen patients with large (>5 cm) high-grade STS were enrolled in the study. They were treated in the neoadjuvant setting with 5,040 cGy of EBRT, split into 28 fractions and delivered 5 days per week, combined with intratumoral injection of 10(7) DCs followed by complete resection. DCs were injected on the second, third, and fourth Friday of the treatment cycle. Clinical evaluation and immunological assessments were performed.

Results

The treatment was well tolerated. No patient had tumor-specific immune responses before combined EBRT/DC therapy; 9 patients (52.9%) developed tumor-specific immune responses, which lasted from 11 to 42 weeks. Twelve of 17 patients (70.6%) were progression free after 1 year. Treatment caused a dramatic accumulation of T cells in the tumor. The presence of CD4(+) T cells in the tumor positively correlated with tumor-specific immune responses that developed following combined therapy. Accumulation of myeloid-derived suppressor cells but not regulatory T cells negatively correlated with the development of tumor-specific immune responses. Experiments with (111)In labeled DCs demonstrated that these antigen presenting cells need at least 48 h to start migrating from tumor site.

Conclusions

Combination of intratumoral DC administration with EBRT was safe and resulted in induction of antitumor immune responses. This suggests that this therapy is promising and needs further testing in clinical trials design to assess clinical efficacy.

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Int J Radiat Oncol Biol Phys. Author manuscript; available in PMC 2014 Nov 23.
Published in final edited form as:
PMCID: PMC4241354
NIHMSID: NIHMS606941
PMID: 21398051

Combination of External Beam Radiation (EBRT) with Intratumoral Injection of Dendritic Cells as Neo-Adjuvant Treatment of High-Risk Soft Tissue Sarcoma Patients

Steven E. Finkelstein, MD,* Cristina Iclozan, PhD,* Marilyn M. Bui, MD,* Matthew J. Cotter, PhD, Rupal Ramakrishnan, PhD, Jamil Ahmed, BS, David R. Noyes, PhD, David Cheong, MD, Ricardo J. Gonzalez, MD, Randy V. Heysek, MD, Claudia Berman, MD, Brianna C. Lenox, BS, William Janssen, PhD, Jonathan S. Zager, MD, Vernon K. Sondak, MD, G. Douglas Letson, MD, Scott J. Antonia, MD, PhD, and Dmitry I. Gabrilovich, MD., PhD.
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Abstract

Purpose

The goal of this study was to determine the effect of combination of intratumoral administration of dendritic cells (DC) and fractionated external beam radiation (EBRT) on tumor-specific immune responses in patients with soft tissue sarcoma (STS).

Methods and Material

Seventeen patients with large (>5 cm) high grade STS were enrolled in the study. They were treated in the neoadjuvant setting with 5040 cGy of EBRT, split into 28 fractions and delivered 5 days a week, combined with intratumoral injection of 107 DCs followed by complete resection. DCs were injected on the second, third, and fourth Friday of the treatment cycle. Clinical evaluation and immunological assessments were performed.

Results

The treatment was well tolerated. No patient had tumor-specific immune responses before combined EBRT/DC therapy; nine patients (52.9%) developed tumor-specific immune responses, which lasted from 11 to 42 weeks. Twelve of 17 patients (70.6%) were progression free after one year. Treatment caused a dramatic accumulation of T cells in the tumor. The presence of CD4+ T cells in the tumor positively correlated with tumor-specific immune responses that developed following combined therapy. Accumulation of myeloid-derived suppressor cells but not regulatory T cells negatively correlated with the development of tumor-specific immune responses. Experiments with 111In labeled DCs demonstrated that these antigen presenting cells need at least 48 hr to start migrating from tumor site.

Conclusions

Combination of intratumoral DC administration with EBRT was safe and resulted in induction of antitumor immune responses. This suggests that this therapy is promising and need further testing in clinical trials design to assess clinical efficacy.

Keywords: sarcoma, dendritic cells, tumor immunity, combined treatment

Introduction

Intratumoral administration of dendritic cells (DC) is one of the promising methods of induction of therapeutic antitumor immune responses. The main advantage of this approach is that a large variety of tumor-associated antigens present in tumors can be utilized. In addition, patients don’t need to be selected or excluded based on HLA type or the expression of specific antigens. However, immunotherapy alone rarely causes curative anti-tumor effects as the manipulation of tumor microenvironment is necessary to potentiate the effect of DC administration1. Ionizing radiation presents one such powerful intervention. Radiation can not only kill tumor cells releasing tumor antigens, but can also exert various immunomodulatory effects including induction of the expression of cytokines, chemokines, and release of inflammatory mediators 2–4. It also increases the permeability of the local vasculature that leads to recruitment of circulating leukocytes into surrounding tissues including antigen-presenting cells and effector T cells5–7. Thus, the proinflammatory microenvironment within irradiated tumors could provide DCs with maturation-inducing stimuli critical for eliciting effective antigen presentation.

In a number of pre-clinical studies we and others have demonstrated that local tumor irradiation in combination with intratumoral DC administration but not irradiation alone resulted in potent antitumor immune responses that translated into an antitumor effect 7–12. These preclinical studies provided a compelling rationale for testing this approach in the clinic. In a phase I clinical trial of hepatoma, intratumoral administration of DCs in combination with a single high dose of conformal radiation was found to be safe and in some cases resulted in induction of tumor-specific immune responses 13, 14. However, it remains unclear whether conventionally fractionated EBRT coupled with DC can cause a robust immune response in a substantial proportion of patients, whether this response correlates with clinical outcome, and whether the treatment affects immune suppressive cells present in cancer patients. In this study we tested this approach in patients with soft tissue sarcomas (STS).

Sarcomas are relatively rare neoplasms with approximately 10,000+ new cases occurring in the United States every year. Many studies have shown that the preoperative radiotherapy and surgery is an effective strategy to treat many STS with high risk features 15–18. Unfortunately, conventional therapy for large, high grade tumors is frequently systemically ineffective, which makes this a very deadly problem. Indeed, despite a multidisciplinary, multimodality approach employing radiation therapy and at times systemic chemotherapy, approximately 50% of patients with large, high grade STS will go on to develop distant metastasis19. Novel approaches to deal with the systemic potential of this disease are needed.

Materials and Methods

Patient selection and treatment

Seventeen patients with histologically confirmed large high-grade STS of the extremity/trunk/chest wall were enrolled to the study (Table 1). These patients had clinical stage T2N0M0 with a significant (>50%) risk of progressing to distant metastases. All patients provided written informed consent to an Institutional Review Board of University of South Florida approved protocol. Treatment schema is shown in Fig. S1. Patients were treated with external beam radiation (EBRT) combined with experimental intratumoral injection of dendritic cells (DC). Patients received 5040 cGy EBRT in 28 equal fractions. Radiation was delivered 5 days a week (Monday-Friday). DCs (107 cells) were injected intatumorally three times on the second, third, and fourth Friday during the course of radiation. One additional DC injection was given several days prior to surgery to assess DC migration (see below). Tumors were surgically resected 3–6 weeks after the completion of EBRT. Peripheral blood from 15 healthy volunteers (10 females and 5 males age 32–48) was used to establish control values in the analysis of cell phenotype.

Table 1

Patients characteristics and clinical effect of the treatment

PatientAgeSexLocationDiagnosisGradeMax Tumor size prior to therapy on MRI (cm)Max Tumor size after therapy on MRI (cm)Tumor size after therapy on pathology (cm)% Tumor necrosis prior to therapy on MRI% Tumor necrosis after therapy on MRITumor necrosis/fibrosis/hyalinization (%) PATH
164FL pelvisUndifferentiated pleomorphic sarcomaHG10.41211101080
281MR flankSpindle cell sarcomaHG172019507070
373FL spineSarcoma with myxoid and chondroid featuresHG7.69.110509053
454MR shoulderMonophasic synovial sarcomaHG545.35510
546FR thighSpindle/pleomorpic cell sarcomaHG10.710.16.5101070
658ML thighUndifferentiated pleomorphic sarcomaHG9.711.511308090
725ML thighMonophasic synovial sarcomaHG88.41004030
879ML armSpindle/pleomorpic cell sarcomaHG76.611101025
1953MR groin/thighSclerosing epthelioid fibrosarcomaHG12.612.2145520
1050MR paraspineUndifferentiated pleomorphic sarcomaHG15.115.115108070
1170ML thighUndifferentiated pleomorphic sarcomaHG12.213.718205070
1273MBackSclerosing epthelioid fibrosarcomaHG69.7120050
1382MR armMyxoid spindle cell sarcomaIG5.94.8509095
1460MR legUndifferentiated pleomorphic sarcomaHG147.77.551040
1569FL kneeSpindle/pleomorpic cell sarcomaHG5.87.39406098
1634ML kneeMalignant peripheral nerve sheath tumorHG6.213.012505070
1741MGroinMyxoid/round cell liposarcomaIG12.18.81301080
Mean9.7210.2411.1417.3539.4160.06
Standard Deviation3.944.164.0818.4033.1828.07

HG – high grade; IG – intermediate grade

Preparation and administration of DCs

DCs were prepared using culture of mononuclear cells with GM-CSF and IL-4 as described previously 20. DC phenotype was defined as lineage (CD3, CD14, CD19, CD20, CD56) negative, HLA-DR positive cells. DCs (107 cells) were injected in a total volume of 1 ml.

Evaluation of DC migration

DCs were labeled with 111In and 5×106 cells were injected intratumorally prior surgery. Patients were split into three groups. One group has received injection of labeled DCs 24 hr before surgery, the second group 48 hr, and the third group 72 hr before surgery. DC labeling was performed at AnazaoHealth Corporation (Tampa, FL). The patients were imaged using a standard gamma camera (Siemens e-cam) at 20 minutes post injection and immediately prior to surgery. The camera has a 20% window at the 171 kev Indium energy peak and a 20% window at the 245 kev Indium energy peak. An intraoperative gamma probe (US Surgical Navigator) was used during surgical dissection for localization purposes. Multiple measurements were performed for each patient and the results were averaged. The ratios of tumor to background, and lymph nodes to background were compared. The ratio of counts of cases performed at different times was compared following correction for decay.

Evaluation of immune responses

Peripheral blood mononuclear cells (MNC) were collected from patients at different time points during the treatment (Fig. S1) and kept frozen at −180°C. All samples from one patient were analyzed simultaneously to reduce inter-experimental variability. Tumor cells were harvested by core biopsies of patients’ tumors prior the treatment. Tumor cell lysates (TCL) were prepared as described 21 by repeated snap freeze-thawing cycles and stored in liquid nitrogen. Lysates from patients MNC were used as controls. T-cell responses to TCL were assessed using IFN-γ ELISPOT22, 23 and proliferation assays.

To evaluate T cell response to survivin, DCs were generated from patients MNC using GM-CSF and IL-4 and infected with adenovirus-survivin (Ad-surv) as described previously to serve as stimulator cells 22. As a control we used an adenoviral construct containing empty vector (Ad-c). An individual patient was considered a responder to TCL or survivin if at any time point the response in the IFN-γ ELIPOT assay was higher than 30 spots per 2×105 cells and in the proliferation assay higher than 3000 CPM AND the response in IFN-γ ELISPOT or proliferation assays to TCL or Ad-surv was more than 2 SD higher than the response to the corresponding control lysate or Ad-c at the same time point AND 2 SD higher than the response to the same stimuli before start of the treatment.

Analysis of cell phenotype

Cell phenotype was evaluated by multicolor flow cytometry using a LSR II flow cytometer and indicated monoclonal antibodies obtained from Becton Dickinson. The following combinations of antibodies were used to identify cell populations:

  1. Total population of DCs: Lineage− (Lin) (CD3, 14, 19, 56) HL-DR+

  2. Mature DCs: Lin−HLA-DR+CD86+

  3. Myeloid-derived suppressor cells (MDSC) Lin− HLA-DR− CD33+ and CD11b+ CD14− CD33+

  4. Regulatory T cells (Treg) CD4+CD25+Foxp3+ and CD4+CD27− Foxp3+ cells.

Dead cells were eliminated from the analysis by using DAPI staining and in samples with intracellular staining by using Live/Dead Aqua solution (Invitrogen, Carlsbad, CA).

Histological and immunohistochemical evaluation of tumor tissues

Each tumor case was confirmed by pre-treatment biopsy with histology, immunohistochemistry, cytogenetic or molecular testing. Therapy response assessment included tumor necrosis, fibrosis and hyalinization as well as cytological changes. The viable tumor cells were estimated as 100% minus % of tumor necrosis/fibrosis/hyalinization.

In immunohistochemical studies the following antibodies were used: survivin (Novus Biological, Littleton, CO), CD3 (Ventana), CD4 (Cell Marque, Rocklin, CA), and CD8 (Ventana). The percentage of the immunoreactive cells from the representative tumor section was counted in at least 10 representative areas.

Statistical Analysis

We utilized GraphPad Prizm software for statistical analyses. The Wilcoxon Mann-Whitney test was used to determine a relationship between two groups with continuous variables. Univariate associations between frequency of responders and groups were analyzed by Fisher’s exact test considering small sample sizes. No attempt was made to adjust to multiple tests in this exploratory study. Any results appearing to be statistically significant require further confirmation. Two-sided tests were used for all calculations. The statistical significance for all analyses was determined with p<0.05.

Results

Clinical response to combination of EBRT with intratumoral DC administration

No significant (>= grade 2) toxicity was observed during combination EBRT/DC neo-adjuvant treatment. Post operative wound complications were observed in 5/17 patients (29.41%) defined using NCIC criteria 16. Twelve patients (70.6%) had no evidence of the disease for at least one year after the start of the treatment (time of follow-up). Among those 12 patients, 6 are disease-free for more than 2 years and 4 patients for more than 3 years. There were no local recurrences. Pathological evaluation of specimens collected during the surgery after completion of the EBRT/DC treatment demonstrated tumor necrosis/fibrosis/hyalinization with infiltration of lymphocytes (Fig. S2).

Immune responses in patients treated with EBRT and DC administration

Nine out of seventeen patients (52.9%) demonstrated a positive response to either TCL or survivin on at least one point after the start of treatment. Among nine responders three patients had immune responses detected at only one time point and six patients had more robust response detected at least at two time points (Table 2). Most of the responses to TCL were first detected 4–6 weeks after start of the treatment and became undetectable by weeks 11–16 (6–11 weeks after finish of the therapy). Immune responses to survivin lasted substantially longer (until weeks 22–42). Examples of immune responses are shown in Fig. 1.

An external file that holds a picture, illustration, etc. Object name is nihms606941f1.jpg
Immune response in patients treated with EBRT and DC administration

A. IFN-γ producing cells in ELISPOT assay. Each experiment was performed in quadruplicate. Patients 12 and 10 were considered as responders, patient 7 as non-responder. B. T-cell proliferation after stimulation with 100 μg control or tumor cell lysates. Cell proliferation was evaluated by uptake of 3[H]-thymidine. Each experiment was performed in triplicate. Patients 7 and 8 were considered as responders, patient 4 as non-responder. C. MNC from indicated patients were stimulated with DCs infected with control adenovirus (Ad-c) or Ad-surv. The number of IFN-γ producing cells was evaluated in ELISPOT assay. Each experiment was performed in quadruplicate. Patients 15 and 8 were considered as responders, patient 16 as non-responder.

Table 2

Immune responses in patients treated with EBRT and DCs

PatientsLysate IFN-γLysate proliferationSurvivin IFN-γOverall response
1NoneNoneNoneNone
2NoneNoneNoneNone
3NoneNoneNoneNone
4NoneNoneNoneNone
5NoneNoneTransientTransient
6NoneNoneNoneNone
7NoneTransientNoneTransient
8NoneRobustTransientRobust
9NoneRobustTransientRobust
10TransientTransientNoneTransient
11NoneNoneRobustRobust
12TransientTransientRobustRobust
13NoneNoneNoneNone
14NoneNoneNoneNone
15NoneNoneRobustRobust
16NoneNoneNoneNone
17TransientRobustNoneRobust

None – no immune response was detected at any time point;

Transient immune response – response detected at only one time point;

Robust immune response – response detected at least at two time points;

We asked whether immune responses to survivin correlated with the level of expression of this protein in tumor cells. Patients were split into two groups based on the survivin expression in tumor samples: 6 patients with low level of expression (less than 20% of tumor cells positive) (Fig. 2A right panel) and 10 patients with high level of expression (more than 20%) (Fig. 2A, left panel). None of 6 patients with a low level of survivin expression in tumor cells had detectable responses to survivin after completion of the treatment, whereas 6 out of 10 patients (60%) with high level of expression developed survivin-specific response to the treatment (p=0.026). No correlation between the level of survivin expression and response to TCL was observed (Fig. 2B).

An external file that holds a picture, illustration, etc. Object name is nihms606941f2.jpg
Immune response to survivin and T-cell infiltration of the tumors

A. Example of staining with anti-survivin antibody of tumor tissues collected prior to start of the treatment. Left panel patient with high expression of surviving (>20%), right panel patient with low expression (<20%). B. Statistical analysis of link between survivin expression in tumors and survivin-specific immune response to the treatment. Patients with less than 20% survivin positive tumor cells were considered “survivin low” and more than 20% as “survivin high”. Non-responders – patients that did not develop immune response to TAA, responders – patients who had detectable response to either TCL or survin. Tumor samples were available from only 16 patients. C. Tumor tissues collected prior to the treatment (left panel) and during the surgery after the treatment (right panel) and stained with anti-CD3 antibody. D. Tumor tissues were collected before start of the treatment and during the surgery (4–5 weeks after the treatment). For the analysis patients were split into three groups: those with robust response to vaccination (n= 5), transient response (n=3) and non-responders (n=5). The number of T-lymphocytes in tumor tissues was evaluated by immunohistochemistry and calculated per high power field (x400) after counting at least 10 randomly selected fields. For each patient group the difference between the number of cells in tumor tissues after the treatment was significantly higher than before (p<0.05). The differences between groups were not statistically significant except as shown in the figure.

Dramatic accumulation of T-lymphocytes in tumor tissues after the treatment was observed (p<0.01) (Fig. 2C, D). Infiltration of CD4+ T cells in tumor tissues from patients who developed an immune response to the treatment was significantly (p=0.026) higher than in patients who did not respond to the treatment (Fig. 2D). No correlation between T cell (including CD4+T cells) infiltration and disease progression was detected.

Correlation of immune responses to the treatment with the levels of immune suppressive myeloid and lymphoid cells

Immune suppressive myeloid cells (MDSC) and lymphocytes (Treg) play a major role in inhibition of immune responses in cancer. We investigated a connection between the levels of MDSC and Tregs and immune responses to TCL and survivin. We used two combinations of markers to identify MDSC: Lin−HLA-DR−CD33+ and CD14−CD11b+CD33+ 24–26. Both of these sets of markers showed similar results. Patients who did not develop immune responses had higher levels of MDSC than healthy volunteers. The differences became significant after start of the treatment. In contrast, the presence of MDSC in patients who developed immune responses remained low. As a result after the treatment the differences in the proportion of MDSC between responders and non-responders were statistically significant (Fig. 3A). The presence of DCs in the patients was not different from control and was similar between responders and non-responders (Fig. 3B). The proportion of Treg in patients was slightly increased. However, this increase was not statistically significant. No differences between responders and non-responders were found (Fig. 3C). Treatment did not significantly affect the absolute number of neutrophils, or lymphocytes. There was only a temporary increase (p=0.03) in the number of monocytes. The differences in the absolute numbers of MDSC, DCs, and Tregs between the groups were similar to those observed in the proportion of those cell populations (Fig. S3).

An external file that holds a picture, illustration, etc. Object name is nihms606941f3.jpg
Cytokines and immune suppressive myeloid and lymphoid cells

A. Proportion of MDSC in patients. Two antibody cocktails (indicated on the graphs) were used to identify MDSC. Cntrl – values in 12 healthy volunteers. * - statistically significant differences from control. P values for statistically significant differences between the groups are shown on the graph. B. Proportion of DCs in cancer patients. C. Proportion of Treg in patient.

In healthy volunteers the upper 95% confidence interval of the proportion of MDSC was 2.678%. To assess correlation between MDSC level and immune responses all patients were split into two groups based on the values of MDSC in healthy donors: patients who had MDSC proportion within 95% CI of healthy control during or after the treatment and patients who had increased level of MDSC during or after the treatment. Of 7 patients with MDSC proportion within the CI of control group, 6 developed immune response to TCL or survivin. Out of 8 patients with elevated level of MDSC only 1 developed immune response (p=0.01). In two patients analysis could not be performed.

Evaluation of DC migration after intatumoral administration

In order to assess the kinetic of DC migration after intratumoral injection DCs were labeled with 111In that is suitable for analysis of DC migration 27. The maximum duration of observation of DC migratory activity depends on the half-life of the radioisotope (72 hr). To minimize inter-patient variability, radioactivity in the center of the tumor was used as a reference point. As a control in each patient we used the ratio of background radioactivity (distant part of the body) to the center of the tumor. Twenty-four hours after injection of DCs no differences between the level of radioactivity at the edge of the tumor or draining lymph nodes and background was detected, indicating lack of measurable DC migration (Fig. 4A.B). In contrast, 48 hr after DC injection significant radioactivity was detected in both edge of the tumor and lymph nodes (p<0.05). It became significantly higher 72 hr after DC injection indicating migration of DCs from the site of injection (Fig. 4B).

An external file that holds a picture, illustration, etc. Object name is nihms606941f4.jpg
Evaluation of DCs migration after injection to the tumor

DCs were labeled with 111In and injected intratumorally at different time prior to surgery. 24 hr group included 5 patients, 48 hr group – 4 patients, and 72 hr group – 6 patients. A. Ratio of radioactivity at the edge of the tumor to the center of the tumor. B. Ratio of radioactivity at the draining lymph nodes to the radioactivity at the center of the tumor. Background – ratio of radioactivity at distant site to the radioactivity at the center of the tumor. P values are shown on the graph

Discussion

Herein, we report the clinical and immunologic results of the first phase I/II trial in humans combining localized fractionated EBRT with intratumoral administration of DCs in patients afflicted with high-risk STS. Treatment was well tolerated by all patients. The frequency of wound complications (29.1%) was similar to that reported for EBRT alone 16, 28. The proportion of patients remaining disease free one year after the treatment (70.6%) was similar to that reported for patients with high-grade large STS 16, 29. Thus, preliminary evaluation indicates that addition of DC injection to EBRT did not worsen side effects and clinical outcome of conventionally fractionated pre-operative EBRT followed by surgery.

One important finding of this study is that no patient had significant tumor-specific immune responses before combined EBRT/DC therapy. Only after treatment the development of tumor-specific immune responses was observed in 52.9% of patients. This trial was not designed to test what the individual components of therapy would induce immune responses in vivo in human; however murine data from our and other groups 7–12 suggest that individually either DC or radiation is not sufficient to generate curative tumor-specific immune response or infiltration of tumor site with T-lymphocytes. We believe that these results are promising enough to warrant further trials.

Adequate evaluation of cancer vaccines is impossible without measuring immune responses to tumor-specific antigens. One of the most common approaches is to use whole tumor cell lysates. However, it is more informative to complement this analysis using defined antigens specific for the tumor cells. Survivin can be very effective in this role. Survivin is an antiapoptotic protein30. Overexpression of survivin in tumor cells results in increased expression of survivin epitopes on the tumor cell surface in association with MHC class I molecules, thus representing targets for CTL 22, 31, 32. Survivin is expressed at a high level in many common human cancers but not in normal, terminally differentiated adult tissues 30, 33. In previous studies the expression of survivin has been evaluated in malignant tissue samples from 63 STS patients as well as from a panel of tumor cell lines 34, 35. High survivin levels were detected in tumor samples from more than 75% of patients with stage II and from more than 90% patients with stage III STS 35. In our study 62.5% of patients had high level of survivin expression in tumors. None of the patients with low or undetectable levels of survivin developed immune responses to this protein, whereas 60% of patients with high levels of survivin expression developed specific immune responses. These results are important since overexpression of survivin in cancer patients correlates with more aggressive disease and poor survival 36, 37. Thus, survivin can serve as a good surrogate marker for immunological monitoring of the treatment.

Intratumoral DC administration during the ongoing EBRT treatment raises the concern that DCs subjected to radiation would be functionally impaired. Mouse experiments have shown rapid migration of injected DC’s from the tumor site to regional nodes 7. There are data that radiation doses below 20 Gy do not affect the antigen-presenting function of DCs in vitro38 and that a radiation dose at 7.5 Gy did not affect DC function in vivo 39. The radiation dose in our trial was 1.8 Gy per fraction, which was substantially lower than the amount needed to inactivate DCs. To minimize the possible damage, DCs were injected on Friday mornings after completing the radiation fraction for that day. The next fraction was administered 72 hours later on Monday afternoon. However, it was important to evaluate DC migration in direct experiments. Our data indicate that 24 hr was not enough for significant numbers of DCs to migrate out of tumors. However, after 48 hr more than 10% of DCs were located at the edge of the tumor and 2% in draining lymph nodes. After 72 hr the amount of radioactivity in draining lymph nodes more than doubled indicating that DCs were able to migrate from tumors into lymph nodes. These data were obtained in the absence of radiation. It is possible that migration of DCs from recently irradiated tumors is higher. Our data also suggested that change in fractionation of EBRT may be warranted to provide more time for DC migration out of tumors.

Thus, our data demonstrate that combination of EBRT and intratumoral DC administration could be a promising method of the treatment of patients with high-risk STS and provide rationale for more extensive clinical studies in this and other types of cancer.

Acknowledgments

This work was supported by grant from “Gateway for Cancer Research (to DIG) This manuscript is dedicated in memory of Erin Bryant, our nurse, colleague, and friend

Footnotes

This work was presented at the 51st ASTRO meeting and was awarded clinical winner of the resident clinical/basic science research award.

Conflict of interest notification

No Actual or potential conflicts of interest exist

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