Abstract
Free full text
Genes transferred by retroviral vectors into normal and mutant myoblasts in primary cultures are expressed in myotubes.
Abstract
Retroviral vectors were used to transfer genes efficiently into rat and dog myoblasts in primary cultures under conditions which permitted the transduced myoblasts to differentiate into myotubes expressing the transferred genes. The transduced myotubes expressed normal markers of differentiation and were morphologically indistinguishable from uninfected myotubes. Retroviral vector-mediated gene transfer was also used to correct a genetic enzyme deficiency in mutant canine muscle cells.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen RE. Muscle cell culture as a tool in animal growth research. Fed Proc. 1987 Feb;46(2):290–294. [Abstract] [Google Scholar]
- Armentano D, Yu SF, Kantoff PW, von Ruden T, Anderson WF, Gilboa E. Effect of internal viral sequences on the utility of retroviral vectors. J Virol. 1987 May;61(5):1647–1650. [Europe PMC free article] [Abstract] [Google Scholar]
- Fishman WH, Goldman SS, DeLellis R. Dual localization of beta-glucuronidase in endoplasmic reticulum and in lysosomes. Nature. 1967 Feb 4;213(5075):457–460. [Abstract] [Google Scholar]
- Gambke B, Rubinstein NA. A monoclonal antibody to the embryonic myosin heavy chain of rat skeletal muscle. J Biol Chem. 1984 Oct 10;259(19):12092–12100. [Abstract] [Google Scholar]
- Haskins ME, Desnick RJ, DiFerrante N, Jezyk PF, Patterson DF. Beta-glucuronidase deficiency in a dog: a model of human mucopolysaccharidosis VII. Pediatr Res. 1984 Oct;18(10):980–984. [Abstract] [Google Scholar]
- Lagunoff D, Nicol DM, Pritzi P. Uptake of beta-glucuronidase by deficient human fibroblasts. Lab Invest. 1973 Oct;29(4):449–453. [Abstract] [Google Scholar]
- Lassar AB, Thayer MJ, Overell RW, Weintraub H. Transformation by activated ras or fos prevents myogenesis by inhibiting expression of MyoD1. Cell. 1989 Aug 25;58(4):659–667. [Abstract] [Google Scholar]
- Miller AD, Buttimore C. Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol Cell Biol. 1986 Aug;6(8):2895–2902. [Europe PMC free article] [Abstract] [Google Scholar]
- Nishimura Y, Rosenfeld MG, Kreibich G, Gubler U, Sabatini DD, Adesnik M, Andy R. Nucleotide sequence of rat preputial gland beta-glucuronidase cDNA and in vitro insertion of its encoded polypeptide into microsomal membranes. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7292–7296. [Europe PMC free article] [Abstract] [Google Scholar]
- Okazaki K, Holtzer H. Myogenesis: fusion, myosin synthesis, and the mitotic cycle. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1484–1490. [Europe PMC free article] [Abstract] [Google Scholar]
- Sharma PM, Reddy GR, Vora S, Babior BM, McLachlan A. Cloning and expression of a human muscle phosphofructokinase cDNA. Gene. 1989 Apr 15;77(1):177–183. [Abstract] [Google Scholar]
- Wolfe JH, Schuchman EH, Stramm LE, Concaugh EA, Haskins ME, Aguirre GD, Patterson DF, Desnick RJ, Gilboa E. Restoration of normal lysosomal function in mucopolysaccharidosis type VII cells by retroviral vector-mediated gene transfer. Proc Natl Acad Sci U S A. 1990 Apr;87(8):2877–2881. [Europe PMC free article] [Abstract] [Google Scholar]
Associated Data
Articles from Molecular and Cellular Biology are provided here courtesy of Taylor & Francis
Full text links
Read article at publisher's site: https://doi.org/10.1128/mcb.10.6.3268-3271.1990
Free after 4 months at mcb.asm.org
http://mcb.asm.org/cgi/reprint/10/6/3268
Free to read at mcb.asm.org
http://mcb.asm.org/cgi/content/abstract/10/6/3268
Citations & impact
Impact metrics
Citations of article over time
Alternative metrics
Discover the attention surrounding your research
https://www.altmetric.com/details/124818907
Article citations
Spin infection enables efficient gene delivery to muscle stem cells.
Biotechniques, 63(2):72-76, 01 Aug 2017
Cited by: 5 articles | PMID: 28803542 | PMCID: PMC5768144
Combination therapies for lysosomal storage disease: is the whole greater than the sum of its parts?
Hum Mol Genet, 20(r1):R54-60, 19 Mar 2011
Cited by: 36 articles | PMID: 21421999 | PMCID: PMC3095053
Review Free full text in Europe PMC
Neonatal intramuscular injection with recombinant adeno-associated virus results in prolonged beta-glucuronidase expression in situ and correction of liver pathology in mucopolysaccharidosis type VII mice.
Hum Gene Ther, 10(1):85-94, 01 Jan 1999
Cited by: 85 articles | PMID: 10022533
Gene therapy for lysosomal storage diseases.
Expert Opin Investig Drugs, 7(10):1673-1682, 01 Oct 1998
Cited by: 3 articles | PMID: 15991909
Regulation of N-acetylgalactosamine 4-sulfatase expression in retrovirus-transduced feline mucopolysaccharidosis type VI muscle cells.
DNA Cell Biol, 18(3):187-195, 01 Mar 1999
Cited by: 2 articles | PMID: 10098600
Go to all (16) article citations
Similar Articles
To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.
Gene transfer into rat airway epithelial cells using retroviral vectors.
Somat Cell Mol Genet, 17(2):185-190, 01 Mar 1991
Cited by: 3 articles | PMID: 1849320
An improved retroviral vector for gene transfer into undifferentiated cells.
Nucleic Acids Res, 17(10):4001, 01 May 1989
Cited by: 15 articles | PMID: 2543963 | PMCID: PMC317899
Effects of recombinant retroviral vector mediated human insulin like growth factor-1 gene transfection on skeletal muscle growth in rat.
Chin Med J (Engl), 119(23):1991-1998, 01 Dec 2006
Cited by: 1 article | PMID: 17199944
Retroviral-mediated transfer of a dystrophin minigene into mdx mouse myoblasts in vitro.
FEBS Lett, 296(2):128-134, 01 Jan 1992
Cited by: 40 articles | PMID: 1733767
Funding
Funders who supported this work.
NIDDK NIH HHS (3)
Grant ID: DK-25759
Grant ID: DK-37602
Grant ID: DK-39812
