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Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma.

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  • 1. Pathology Division, National Cancer Center Research Institute, Tokyo, Japan.
      Authors
    • Tsuda H 1
    (1 author)

Proceedings of the National Academy of Sciences of the United States of America, 01 Sep 1990, 87(17):6791-6794
https://doi.org/10.1073/pnas.87.17.6791 PMID: 2168560 PMCID: PMC54623

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Abstract 

Loss of heterozygosity on chromosome 16 is a common genetic alteration in human hepatocellular carcinoma (HCC). To clarify the pathogenetic significance of allele loss on chromosome 16, we performed restriction fragment length polymorphism analysis of 70 surgically resected tumors by using 15 polymorphic DNA markers for chromosome 16. Loss of heterozygosity on chromosome 16 was detected in 36 (52%) of 69 informative cases, and the common region of allele loss in these 36 tumors was located between the HP locus (16q22.1) and the CTRB locus (16q22.3-q23.2). These losses occurred more frequently in HCCs of poor differentiation, of larger size, and with metastasis, whereas they were not detected in HCC at the earliest stage. In addition, these losses were not associated with presence or absence of hepatitis B virus DNA integration or hepatitis C virus infection. These results show that loss of heterozygosity on chromosome 16 is a late event occurring after hepatocarcinogenesis and strongly suggest that this phenomenon is involved in enhancement of tumor aggressiveness during progression of HCC.

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Proc Natl Acad Sci U S A. 1990 Sep; 87(17): 6791–6794.
PMCID: PMC54623
PMID: 2168560

Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma.

H Tsuda, W D Zhang, Y Shimosato, J Yokota, M Terada, T Sugimura, T Miyamura, and S Hirohashi
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Abstract

Loss of heterozygosity on chromosome 16 is a common genetic alteration in human hepatocellular carcinoma (HCC). To clarify the pathogenetic significance of allele loss on chromosome 16, we performed restriction fragment length polymorphism analysis of 70 surgically resected tumors by using 15 polymorphic DNA markers for chromosome 16. Loss of heterozygosity on chromosome 16 was detected in 36 (52%) of 69 informative cases, and the common region of allele loss in these 36 tumors was located between the HP locus (16q22.1) and the CTRB locus (16q22.3-q23.2). These losses occurred more frequently in HCCs of poor differentiation, of larger size, and with metastasis, whereas they were not detected in HCC at the earliest stage. In addition, these losses were not associated with presence or absence of hepatitis B virus DNA integration or hepatitis C virus infection. These results show that loss of heterozygosity on chromosome 16 is a late event occurring after hepatocarcinogenesis and strongly suggest that this phenomenon is involved in enhancement of tumor aggressiveness during progression of HCC.

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Selected References

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