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Insufficient glucose supply is linked to hypothermia upon cold exposure in high-fat diet-fed mice lacking PEMT.

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Affiliations

  • 1. Group on the Molecular and Cell Biology of Lipids University of Alberta, Edmonton, Canada Departments of Biochemistry, University of Alberta, Edmonton, Canada.
      Authors
    • Gao X 1
    • van der Veen JN 1
    • Vance DE 1
    (3 authors)
  • 2. Departments of Biochemistry, University of Alberta, Edmonton, Canada.
      Authors
    • Fernandez-Patron C 2
    (1 author)
  • 3. Group on the Molecular and Cell Biology of Lipids University of Alberta, Edmonton, Canada Medicine, University of Alberta, Edmonton, Canada.
      Authors
    • Vance JE 3
    (1 author)
  • 4. Group on the Molecular and Cell Biology of Lipids University of Alberta, Edmonton, Canada Agricultural, Food, and Nutritional Science, University of Alberta, Edmonton, Canada.
      Authors
    • Jacobs RL 4
    (1 author)

ORCIDs linked to this article

Journal of Lipid Research, 25 Jun 2015, 56(9):1701-1710
https://doi.org/10.1194/jlr.m059287 PMID: 26113536 PMCID: PMC4548774

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Abstract 

Mice that lack phosphatidylethanolamine N-methyltransferase (Pemt(-/-) mice) are protected from high-fat (HF) diet-induced obesity. HF-fed Pemt(-/-) mice show higher oxygen consumption and heat production, indicating that more energy might be utilized for thermogenesis and might account for the resistance to diet-induced weight gain. To test this hypothesis, HF-fed Pemt(-/-) and Pemt(+/+) mice were challenged with acute cold exposure at 4°C. Unexpectedly, HF-fed Pemt(-/-) mice developed hypothermia within 3 h of cold exposure. In contrast, chow-fed Pemt(-/-) mice, possessing similar body mass, maintained body temperature. Lack of PEMT did not impair the capacity for thermogenesis in skeletal muscle or brown adipose tissue. Plasma catecholamines were not altered by Pemt genotype, and stimulation of lipolysis was intact in brown and white adipose tissue of Pemt(-/-) mice. HF-fed Pemt(-/-) mice also developed higher systolic blood pressure, accompanied by reduced cardiac output. Choline supplementation reversed the cold-induced hypothermia in HF-fed Pemt(-/-) mice with no effect on blood pressure. Plasma glucose levels were ∼50% lower in HF-fed Pemt(-/-) mice compared with Pemt(+/+) mice. Choline supplementation normalized plasma hypoglycemia and the expression of proteins involved in gluconeogenesis. We propose that cold-induced hypothermia in HF-fed Pemt(-/-) mice is linked to plasma hypoglycemia due to compromised hepatic glucose production.

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Logo of jlrJournal of Lipid Research
J Lipid Res. 2015 Sep; 56(9): 1701–1710.
PMCID: PMC4548774
PMID: 26113536

Insufficient glucose supply is linked to hypothermia upon cold exposure in high-fat diet-fed mice lacking PEMT[S]

Xia Gao,*† Jelske N. van der Veen,*† Carlos Fernandez-Patron,† Jean E. Vance,*§ Dennis E. Vance,*† and René L. Jacobs1,*,**
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Abstract

Mice that lack phosphatidylethanolamine N-methyltransferase (Pemt−/− mice) are protected from high-fat (HF) diet-induced obesity. HF-fed Pemt−/− mice show higher oxygen consumption and heat production, indicating that more energy might be utilized for thermogenesis and might account for the resistance to diet-induced weight gain. To test this hypothesis, HF-fed Pemt−/− and Pemt+/+ mice were challenged with acute cold exposure at 4°C. Unexpectedly, HF-fed Pemt−/− mice developed hypothermia within 3 h of cold exposure. In contrast, chow-fed Pemt−/− mice, possessing similar body mass, maintained body temperature. Lack of PEMT did not impair the capacity for thermogenesis in skeletal muscle or brown adipose tissue. Plasma catecholamines were not altered by Pemt genotype, and stimulation of lipolysis was intact in brown and white adipose tissue of Pemt−/− mice. HF-fed Pemt−/− mice also developed higher systolic blood pressure, accompanied by reduced cardiac output. Choline supplementation reversed the cold-induced hypothermia in HF-fed Pemt−/− mice with no effect on blood pressure. Plasma glucose levels were ~50% lower in HF-fed Pemt−/− mice compared with Pemt+/+ mice. Choline supplementation normalized plasma hypoglycemia and the expression of proteins involved in gluconeogenesis. We propose that cold-induced hypothermia in HF-fed Pemt−/− mice is linked to plasma hypoglycemia due to compromised hepatic glucose production.

Keywords: adipose tissue, obesity, phosphatidylcholine, phosphatidylethanolamine, phosphatidylethanolamine N-methyltransferase, hypertension

Phosphatidylethanolamine N-methyltransferase (PEMT) catalyzes phosphatidylcholine (PC) biosynthesis via three sequential methylations of phosphatidylethanolamine (PE) (1). In the liver, PEMT contributes ~30% of hepatic PC production, whereas the remaining 70% of hepatic PC is synthesized via the CDP-choline pathway (2, 3). Normal levels of hepatic PC are required to maintain membrane integrity (4) and normal VLDL secretion (5–7). In mice, inhibition of either the CDP-choline pathway by liver-specific deletion of CTP:phosphocholine cytidylyltransferase (LCT)α or elimination of the PEMT pathway reduces hepatic PC content by ~30% and causes hepatic steatosis (8). The importance of the PEMT pathway has been highlighted in Pemt−/− mice fed either a choline-deficient diet (4) or a high-fat (HF) diet (9). When fed the HF diet for 10 weeks, Pemt−/− mice develop nonalcoholic steatohepatitis, but are protected against diet-induced obesity (DIO) and insulin resistance (9). HF-fed Pemt−/− mice also exhibit higher oxygen consumption than HF-fed Pemt+/+ mice (9). LCTα−/− mice fed the HF diet also develop steatohepatitis (8–10), but are not protected from DIO (9). Thus, the resistance of Pemt−/− mice to DIO does not appear to rely primarily on decreased hepatic PC or impaired VLDL secretion (5, 6).

Increased oxygen consumption and heat production in HF-fed Pemt−/− mice (9) suggest thermogenesis in these mice might contribute to adipose hypotrophy in responding to the HF diet (9, 11). Cold exposure is widely used to determine the capability for thermogenesis (12). Upon cold exposure, both skeletal muscle-mediated shivering thermogenesis and brown adipose tissue (BAT)-mediated nonshivering thermogenesis are activated to maintain the core body temperature (12). Intolerance to cold exposure might reflect inadequate heat production or a defective heart to accommodate the cold-activated catabolism (12).

In this study, we investigated the contribution of thermogenesis to the resistance of Pemt−/− mice to DIO. After consuming the HF diet for 2 weeks, Pemt−/− mice unexpectedly failed to maintain body temperature above 28°C during acute cold challenge. On the other hand, Pemt−/− mice that were fed a standard chow diet were able to maintain their body temperature over 33°C upon cold exposure. We explored the mechanisms underlying cold-induced hypothermia in HF-fed Pemt−/− mice. Dietary choline supplementation completely reversed the hypothermia in HF-fed Pemt−/− mice, with concomitant normalization of plasma glucose and markers of hepatic gluconeogenesis.

MATERIALS AND METHODS

Animal handling and diets

All procedures were approved by the University of Alberta’s Institutional Animal Care Committee in accordance with guidelines of the Canadian Council on Animal Care. Male C57Bl/6 Pemt+/+ and Pemt−/− mice were housed with free access to water and chow (LabDiet, #5001). Pemt+/+ and Pemt−/− mice (8–9 weeks old) were fed a HF diet (60% calories from fat; Bio-Serv, #F3282) or choline-supplemented HF (HFCS) diet for 2 weeks. The HF diet contained 1.15 g choline per kg diet, and choline content was raised to 4 g choline per kg diet in the HFCS diet. Tissues were collected from mice that had either been fasted for 12 h or not fasted, as indicated. Blood was collected by cardiac puncture. Tissues were either used immediately for assessment of fatty acid oxidation or stored at −80°C.

The procedure for cold challenge was adapted from a previous study (13). Mice were fasted for 4 h then transferred to the cold room (4°C) with individual housing and no bedding, food, or water for up to 4 h. Rectal body temperature was determined hourly using a mouse rectal probe (model 4600 Precision thermometer). When the body temperature dropped below 28°C, the mice were removed from the cold room. Tissues were collected immediately after the cold exposure.

In vivo metabolic analysis

Metabolic measurements (heat production and beam breaks) were obtained using a comprehensive lab animal monitoring system (Columbus Instruments, Columbus, OH) as described (9). Mice were housed individually for 3 days before being placed in metabolic cages. Following an initial 24 h acclimatization period, measurements were taken every 13 min for 24 h.

Analytical procedures

Plasma TG and TG extracted from tissue (liver or interscapular BAT) homogenates were quantified using a commercially available kit from Roche Diagnostics. Plasma NEFAs and glucose were quantified using commercially available kits from Wako Chemicals GmbH and BioAssay Systems (14), respectively. Plasma thyroid hormones [triiodothyronine (T3) and thyroxine (T4)] were quantified by commercially available kits (Leinco Technologies, Inc.). Plasma epinephrine and norepinephrine were quantified with a commercially available 2-CAT (A-N) Research ELISA kit (Labor Diagnostika Nord). PC and PE extracted from BAT homogenates were quantified as previously described (15). Protein concentrations were determined by the Bradford assay (Bio-Rad). A portion of BAT was fixed in 10% buffered formalin and subjected to hematoxylin and eosin staining.

Fatty acid oxidation

The rate of fatty acid oxidation in interscapular BAT or gastrocnemius muscle was determined as described with minor modifications (11, 16). Pemt+/+ and Pemt−/− mice were fed the HF diet for 2 weeks. After 12 h fasting, freshly isolated interscapular BAT or gastrocnemius muscle was gently homogenized in ice-cold buffer [10 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA (pH 7.4)] followed by sonication for 10 s. After centrifugation at 500 g for 10 min at 4°C, the supernatant was collected and protein concentration was determined. The rate of [1-14C]palmitate oxidation was determined over 1 h by capturing the amount of 14CO2 released (complete oxidation) and by measuring the amount of 14C-labeled acid-soluble metabolites (incomplete oxidation). Briefly, 1.5–2 mg BAT homogenate or 0.35–0.55 mg muscle homogenate protein (in 250 μl) was preincubated with the reaction mixture at 37°C for 5 min in a 25 ml glass vial. The glass vial was fitted with an Eppendorf tube containing Whatman filter paper (for injection of 150 μl of 1 M NaOH to capture the released CO2) and was capped with a rubber stopper. The assay mixture contained 115 mM NaCl, 2.6 mM KCl, 10 mM Tris-HCl (pH 7.4), 1.2 mM KH2PO4, 10 mM NaHCO3, 0.2 mM EDTA, 0.3% fatty acid-free BSA, 2 mM L-carnitine, 5 mM ATP, 0.5 mM malate, and 0.1 mM CoA. The reaction was initiated by injecting BSA-conjugated 0.2 mM palmitate (molar ratio 6:1 for palmitate:BSA), which contained 0.5 μCi/ml 1-14C-palmitate, through the rubber stopper. After 1 h of incubation at 37°C, 300 μl of 3 M perchloric acid was injected to stop the reaction. The vials were left at room temperature for 2 h to capture the released CO2. The radioactivity of CO2 captured by the filter papers and the radioactivity in acid-soluble metabolites (in the supernatant) were measured by a scintillation counter.

Immunoblotting

Liver and interscapular BAT were homogenized in buffer [100 mM Tris-HCl, 150 mM sodium chloride, 1 mM EDTA, 1 mM DTT, and 0.1 mM PMSF (pH 7.4) containing a protease inhibitor cocktail. Gastrocnemius muscle was homogenized in buffer [50 mM Tris-HCl, 250 mM sucrose, 1 mM EDTA, 1 mM DTT, and 0.1 mM PMSF (pH 7.4)] containing a protease inhibitor cocktail. Proteins were transferred to a polyvinylidene difluoride membrane and the membrane was probed with primary antibodies against fatty acid binding protein 4 (FABP4) (Cell Signaling, #2120), PPARγ (Cell Signaling, #24443), CCAAT/enhancer-binding protein β (C/EBPβ) (Santa Cruz, sc150), PPARγ coactivator 1α (PGC1α) (Abcam, #ab54481), phosphoenolpyruvate carboxykinase (PEPCK) (Abcam, #ab70358), uncoupling protein (UCP)1 (Abcam, #ab10983), UCP2 (Abcam, #ab67241), UCP3 (Abcam, #ab3477), porin (VDAC; Abcam, #ab14734), complexes of the electron transport chain (Abcam, #ab110413), calnexin (Enzo Life Sciences, SPA-865), hormone-sensitive lipase (HSL) (Cell Signaling, #4107), phospho-HSL (Ser660; Cell Signaling, #4126), adipose TG lipase (ATGL) (Cell Signaling, #2138), TG hydrolase (TGH) (a gift from Dr. Richard Lehner), protein disulfide isomerase (PDI) (Enzo Life Sciences, SPA-890), or GAPDH (Abcam, #ab8245). The immunoreactive bands were visualized by enhanced chemiluminescence (Amersham Biosciences) according to the manufacturer’s instructions and protein levels were quantified using ImageJ software.

Real-time quantitative PCR and analysis of mitochondrial DNA content

RNA isolation, cDNA synthesis, and real-time quantitative PCR were performed as described previously (17). mRNA levels were normalized to β-actin mRNA; primer sequences are listed in supplementary Table 1. Mitochondrial DNA content was determined as described previously (17). Briefly, total DNA was extracted from livers using a DNEasy kit (Qiagen). The mitochondrial DNA copy number was calculated using real-time quantitative PCR by measuring mitochondrial DNA encoding mitochondrial NADH-ubiquinone oxidoreductase chain 1 versus a nuclear DNA encoding lipoprotein lipase.

Systolic blood pressure

Systolic blood pressure was measured indirectly using a commercially available computerized tail cuff plethysmography system (Kent Scientific Corporation, Torrington, CT) (18). Transthoracic echocardiography was performed as described (19) on mildly anesthetized mice using a Vevo 770 high-resolution imaging system equipped with a 30 MHz transducer (RMV-707b; VisualSonics, Toronto, Canada).

Statistical analysis

Data are expressed as mean ± SEM. Comparisons between two groups were performed using Student’s t-test. For all other comparisons, a two-way ANOVA followed by a Bonferroni post hoc test of individual group differences was used. P < 0.05 was considered significant. Unless otherwise indicated, five to seven animals were used per group.

RESULTS

HF-fed Pemt−/− mice are hypothermic upon cold exposure

When fed the HF diet for 10 weeks, Pemt−/− mice exhibit higher oxygen consumption than Pemt+/+ mice (9), indicating that higher energy expenditure for heat production might account for the resistance of HF-fed Pemt−/− mice to DIO. After 2 weeks of HF feeding, Pemt−/− mice showed higher heat production and physical activity, assessed by counts of beam breaks, than Pemt+/+ mice in the dark cycle only, not the light cycle (Fig. 1A, B). The higher heat production and physical activity might account for the resistance to DIO in Pemt−/− mice. Thus, Pemt−/− and Pemt+/+ mice were fed the HF diet for 2 weeks in the current study. Pemt−/− mice did not gain weight after HF feeding, compared with ~3.7 g body weight gain in Pemt+/+ mice (Fig. 1C). To determine the capacity for thermogenesis, we exposed HF-fed Pemt−/− and Pemt+/+ mice to an acute cold exposure. Unexpectedly, HF-fed Pemt−/− mice were unable to maintain their body temperature upon cold exposure, as the rectal body temperature rapidly (within 2 to 3 h) decreased below 28°C (Fig. 1D). In contrast, Pemt+/+ mice maintained their body temperature above 30°C throughout 4 h cold exposure (Fig. 1D).

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HF-fed Pemt−/− mice are hypothermic upon cold exposure. Pemt+/+ and Pemt−/− mice (8–9 weeks old) were fed the HF diet for 2 weeks. A, B: Mice were housed individually for 3 days then placed in metabolic cages for metabolic measurements throughout the light and dark cycles. Heat production (A) and physical activity (B) accessed by beam breaks. b.w., body weight. C: HF-induced body weight gain. D: Rectal temperature (Temp.) during cold challenge. Mice were fasted for 4 h then placed at 4°C without bedding, food, or water. All data are from five to six mice of each genotype; *P < 0.05 by Student t-test for Pemt+/+ versus Pemt−/−.

The difference in body weight between Pemt−/− and Pemt+/+ mice might have caused the cold-induced hypothermia in HF-fed Pemt−/− mice. However, chow-fed Pemt−/− and Pemt+/+ mice, who had negligible body weight differences (Fig. 2A), maintained their body temperature above 33°C for up to 4 h of cold exposure (Fig. 2B). Thus, upon cold exposure, HF-fed Pemt−/− mice develop hypothermia, which is not caused by lower body weight.

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Chow-fed Pemt−/− mice do not develop hypothermia upon cold exposure. Pemt+/+ and Pemt−/− mice (10–11 weeks old) were fed a standard chow diet. Body weight (A) and rectal temperature (Temp.) (B) during cold challenge. Mice were fasted for 4 h then placed at 4°C without bedding, food, or water. All data are from six to seven mice of each group; *P < 0.05 by Student t-test for Pemt+/+ versus Pemt−/−.

PEMT deficiency does not impair oxidative capacity of skeletal muscle

Cold exposure of mice stimulates shivering thermogenesis, which is mainly mediated by skeletal muscle, to maintain their core body temperature (12). We thus examined the thermogenic capacity of skeletal muscle, freshly dissected from Pemt+/+ and Pemt−/− mice fed the HF diet for 2 weeks, without being exposed to cold. The rate of incomplete fatty acid oxidation, as measured by 14C incorporation into acid soluble metabolites in gastrocnemius muscle from Pemt−/− mice, was not different from that in Pemt+/+ mice, whereas the rate of complete fatty acid oxidation, as measured by 14CO2 generation, was higher in Pemt−/− mice than in Pemt+/+ mice (Fig. 3A). The amounts of mitochondrial marker protein VDAC and electron transport chain proteins (complexes I to V) were the same in Pemt−/− and Pemt+/+ mice (Fig. 3B). Furthermore, levels of UCP2 and UCP3 in Pemt−/− mice were comparable to those in Pemt+/+ mice (Fig. 3B). Thus, Pemt−/− mice seem to have intact oxidative capacity in skeletal muscle.

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Oxidative capacity of skeletal muscle from HF-fed Pemt−/− mice and Pemt+/+ mice. Pemt+/+ and Pemt−/− mice (8–9 weeks old) were fed the HF diet for 2 weeks. Gastrocnemius muscle was collected from mice after an overnight fast, without being exposed to cold. A: The rate of complete (14CO2) and incomplete (14C-ASM) fatty acid oxidation was measured in skeletal muscle homogenates. Data are from five to seven mice of each group; *P < 0.05 by Student t-test for Pemt+/+ versus Pemt−/−. B: Immunoblots of VDAC, electron transport chain complexes I to V, UCP2, UCP3, and the loading control, GAPDH. Data are from three mice of each group.

PEMT deficiency does not alter thermogenic features of BAT

BAT-mediated thermogenesis is promoted by cold exposure. We considered the possibility that defective BAT-mediated thermogenesis contributed to hypothermia in HF-fed Pemt−/− mice. Thus, we examined the thermogenic capacity in BAT. PEMT protein (supplementary Fig. 1A) and its enzymatic activity (data not shown) were undetectable in BAT. When fed a standard chow diet, BAT from Pemt−/− mice contained the same amount of PC (61.05 ± 2.46 nmol/mg protein vs. 61.36 ± 2.31 nmol/mg protein in Pemt+/+ mice) and TG (1.89 ± 0.15 µmol/mg protein vs. 2.11 ± 0.11 nmol/mg protein in Pemt+/+ mice) as Pemt+/+ mice. When the mice were fed the HF diet for 2 weeks, the mass of BAT was the same between genotypes (Fig. 4A). The levels of PC and PE, and the PC/PE ratio, in BAT were not different between Pemt−/− and Pemt+/+ mice (supplementary Fig. 1B). However, BAT from Pemt−/− mice contained slightly less TG than did BAT from Pemt+/+ mice under nonfasting conditions (supplementary Fig. 1C). Fasting greatly reduced TG storage to a similar extent in both genotypes, as shown in histological analysis and biochemical measurement (Fig. 4B, supplementary Fig. 1C), indicating that fasting-induced lipolysis was intact in BAT from Pemt−/− mice.

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Comparable oxidative capacity in BAT from HF-fed Pemt−/− mice and Pemt+/+ mice. Pemt+/+ and Pemt−/− mice (8–9 weeks old) were fed the HF diet for 2 weeks. BAT was collected from mice that had been fasted overnight. A: Weight of BAT as percent body weight (B.W.). B: Hematoxylin and eosin staining of BAT; bar: 50 µm. C: Representative immunoblots of C/EBPβ (indicated by the star), FABP4, PPARγ, and their densitometric analysis relative to the loading control, calnexin. D: Representative immunoblots of PGC1α, UCP1, VDAC, and electron transport chain complexes I to V and their densitometric analysis relative to the loading control calnexin. E: Mitochondrial copy number was assessed as the ratio of mitochondrial NADH-ubiquinone oxidoreductase chain 1 (Nd1) versus a nuclear DNA encoding lipoprotein lipase (Lpl). F: The rate of complete (14CO2) and incomplete (14C-ASM) fatty acid oxidation in BAT homogenates. All data are from five to seven mice of each group; *P < 0.05 by Student t-test for Pemt+/+ versus Pemt−/−.

Lack of PEMT did not significantly impair adipocyte differentiation in BAT. The protein levels of adipocyte differentiation markers PPARγ and FABP4 were the same in HF-fed Pemt−/− mice and Pemt+/+ mice, but the amount of C/EBPβ protein was reduced by 56% by PEMT deficiency (Fig. 4C). However, in mice fed a standard chow diet, the protein levels of FABP4 and C/EBPβ were not different between Pemt genotypes (data not shown). Thus, PEMT deficiency does not impair the differentiation of brown adipocytes. The reduced amount of C/EBPβ protein in HF-fed Pemt−/− mice suggests that lipogenesis might be reduced, which might account for the minor reduction of TG in BAT under nonfasting conditions (supplementary Fig. 1C).

We next explored the thermogenic capacity of BAT. The protein levels of UCP1 and PGC1α, a key regulator of oxidative metabolism and UCP1-mediated thermogenesis in BAT (20), were not different between genotypes (Fig. 4D). Moreover, amounts of the mitochondrial marker protein VDAC and mitochondrial electron transport chain proteins (complexes I to V) were the same in BAT from Pemt−/− and Pemt+/+ mice (Fig. 4D). PEMT deficiency did not alter mitochondrial number either (Fig. 4E). Furthermore, the rates of complete fatty acid oxidation (measured as the amount of released 14CO2) and incomplete fatty acid oxidation (measured as the amount of 14C-labeled acid-soluble metabolites) in BAT from Pemt−/− mice were similar to those in Pemt+/+ mice (Fig. 4F). Overall, BAT of HF-fed Pemt−/− mice and Pemt+/+ mice exhibits comparable thermogenic features.

Cold-stimulated adrenergic activation of lipolysis in adipose tissues is intact in Pemt−/− mice

Cold activates the sympathetic nervous system, particularly via the release of norepinephrine, which stimulates lipolysis in white adipose tissue (WAT) to provide substrates for thermogenesis to maintain core body temperature (21). However, after cold exposure, plasma levels of epinephrine and norepinephrine were not lower in Pemt−/− mice than in Pemt+/+ mice (Fig. 5A). The lipases HSL and ATGL contribute over 95% of lipolytic activity in adipose tissue (22). Following cold exposure, WAT from Pemt−/− mice contained 1.7-fold more phospho-HSL and the same amount of total HSL (Fig. 5B) as did Pemt+/+ mice. Thus, the ratio of phospho-HSL/HSL in WAT of Pemt−/− mice was higher than in Pemt+/+ mice, indicating more activated HSL in Pemt−/− mice compared with Pemt+/+ mice. The amounts of ATGL protein and another lipolytic enzyme, TGH, in WAT from Pemt−/− mice were also higher than in Pemt+/+ mice (Fig. 5B). Consequently, the levels of fatty acids in plasma were elevated by cold exposure in both Pemt−/− and Pemt+/+ mice to a similar level (Fig. 5C), compared with mice not exposed to cold (11). Thus, the exacerbation of cold-induced hypothermia in Pemt−/− mice does not appear to be caused by inefficient lipolysis in WAT.

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Capacity for cold-stimulated TG lipolysis in WAT. Pemt+/+ and Pemt−/− mice (8–9 weeks old) were fed the HF diet for 2 weeks followed by a 4 h fast and cold exposure. A: Plasma levels of epinephrine (E) and norepinephrine (NE). B: Representative immunoblots of lipolytic enzymes: phospho-HSL (p-HSL), total-HSL (HSL), ATGL, TGH, and their densitometric analysis relative to the loading control GAPDH. C: Levels of plasma NEFAs. All data are from five to seven mice of each group; *P < 0.05 by Student t-test for Pemt+/+versus Pemt−/−.

Following cold exposure, the protein levels of phospho-HSL, total-HSL, and ATGL in BAT from Pemt−/− mice were indistinguishable from those in Pemt+/+ mice (supplementary Fig. 2A). In contrast, the amount of TGH protein was lower in BAT from Pemt−/− mice than from Pemt+/+ mice (supplementary Fig. 2A). Cold exposure reduced the amount of TG in BAT from mice of both genotypes compared with BAT collected from nonfasted mice (supplementary Figs. 1C, 2B). After cold exposure, however, BAT from Pemt−/− mice contained 49% less TG than did BAT from Pemt+/+ mice (supplementary Fig. 2B). In addition, amounts of the mitochondrial protein VDAC and mitochondrial number (supplementary Fig. 2C, D) were not influenced by Pemt genotype. The level of PGC1α in BAT from Pemt−/− mice was slightly lower than that in Pemt+/+ mice, whereas the amount of UCP1 protein was 2-fold higher in the Pemt−/− mice (supplementary Fig. 2C). Moreover, the amounts of mitochondrial electron transport chain proteins were equivalent in BAT from Pemt+/+ and Pemt−/− mice, except for a slightly higher amount of complex V in the Pemt−/− mice (supplementary Fig. 2C). Furthermore, compared with Pemt+/+ mice, BAT from Pemt−/− mice contained higher levels of mRNAs encoding proteins involved in fatty acid oxidation, such as carnitine palmitoyltransferase 1α (CPT1α) and long-chain acyl-CoA dehydrogenase (LCAD) (supplementary Fig. 2E). The amounts of mRNAs encoding medium-chain acyl-CoA dehydrogenase (MCAD) and pyruvate dehydrogenase kinase isozyme 4 (PDK4) were not different between genotypes (supplementary Fig. 2E). Thus, in Pemt−/− mice, the HF-induced hypothermia is not the result of impaired adrenergic activation of BAT.

Systolic hypertension and reduced cardiac output in HF-fed Pemt−/− mice

Cold exposure stimulates cardiac output and blood flow to BAT (23, 24). To determine whether the cold-induced hypothermia in HF-fed Pemt−/− mice could be attributed to an insufficient circulatory system, we measured systolic blood pressure in Pemt+/+ and Pemt−/− mice with a computerized tail cuff plethysmography system (18). Systolic blood pressure in HF-fed Pemt−/− mice was 20% higher than in HF-fed Pemt+/+ mice (Fig. 6A), but heart weight was the same in mice of both genotypes (Fig. 6B). Cardiac function of these mice was evaluated by echocardiography (supplementary Methods). In Pemt−/− mice, the ejection fraction, fraction shortening, and cardiac output were 17, 21, and 25% lower than in Pemt+/+ mice (Fig. 6C). However, heart rate was not altered by Pemt genotype (supplementary Table 2). Furthermore, Pemt−/− mice exhibited lower mitral early and late ventricular filling velocities, suggesting that Pemt−/− mice had dias t olic dysfunction and longer left ventricle isovolumic contraction time than Pemt+/+ mice (supplementary Table 2). These data indicate that the HF-induced hypothermia in Pemt−/− mice might be linked to hypertension, accompanied by reduced cardiac output.

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Blood pressure and cardiac output in HF-fed Pemt+/+ and Pemt−/− mice. The mice were fed the HF diet for 2 weeks. A: Systolic blood pressure was measured by a computerized tail cuff plethysmography system. B: Heart mass as percent of body weight (B.W.) and as percent of tibia length (in centimeters). C: In vivo cardiac function was determined by echocardiography: left ventricle ejection fraction, fraction shortening, and cardiac output. All data are from five to six mice of each genotype; *P < 0.05 by Student t-test for Pemt+/+ versus Pemt−/−.

Choline supplementation prevents cold-induced hypothermia in HF-fed Pemt−/− mice

Our previous studies have revealed that Pemt−/− mice are resistant to DIO due to choline insufficiency (9). HF-induced steatohepatitis in Pemt−/− mice is also improved by increased dietary choline (10). To determine whether the hypothermia in HF-fed Pemt−/− mice was also related to choline insufficiency, we increased the amount of choline to 4 g/kg diet in the HF diet that we fed for 2 weeks. Remarkably, choline supplementation completely prevented the hypothermia in Pemt−/− mice (Fig. 7A). All Pemt+/+ mice that were fed either the HF or the HFCS diet maintained their body temperature upon cold exposure. Moreover, the HFCS diet increased body weight in Pemt−/− mice (Fig. 7B) without increasing the weight of BAT or WAT (Fig. 7C, D). In Pemt+/+ mice, the HFCS diet did not alter body weight gain or the mass of BAT or WAT (Fig. 7C, D). The heart weight was not different among the four groups of mice (Fig. 7E). Moreover, the HFCS diet increased blood pressure in Pemt+/+ mice, but not in Pemt−/− mice (Fig. 7G), implying that the higher blood pressure was not a causative factor for the hypothermia. These data demonstrate that choline supplementation prevents cold-induced hypothermia in HF-fed Pemt−/− mice, whereas the higher blood pressure in the HF-fed Pemt−/− mice does not contribute to this process.

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Choline supplementation prevents hypothermia in HF-fed Pemt−/− mice. Pemt+/+ and Pemt−/− mice were fed the HF or HFCS diet for 2 weeks followed by a 4 h fast and cold exposure. A: Rectal temperature during cold challenge. B: Body weight (B.W.) gain over the 2 weeks of feeding. C: BAT weight as percent of B.W. D: WAT weight as percent of B.W. E: Heart weight as percent of B.W. F: Systolic blood pressure in mice fed the HF or HFCS diet for 2 weeks without fasting or cold exposure. All data are from four to seven mice of each group; *P < 0.05 by two-way ANOVA for Pemt+/+ versus Pemt−/−; #P < 0.05 by two-way ANOVA for the HF diet versus the HFCS diet for the same genotype.

Because thyroid hormones are also regulators of thermogenesis (21), we measured plasma levels of thyroid hormones in Pemt+/+ and Pemt−/− mice fed the HF diet or the HFCS diet. In comparison to HF-fed Pemt+/+ mice, the level of plasma T4 was 38% higher in HF-fed Pemt−/− mice, and this increase was prevented by choline supplementation (supplementary Fig. 3A). However, the level of plasma T3, the more active form of thyroid hormone, was 69% lower in Pemt−/− mice than in Pemt+/+ mice, and this difference was not eliminated by choline supplementation (supplementary Fig. 3A). Thus, cold-induced hypothermia in HF-fed Pemt−/− mice is unlikely to be due to insufficient thyroid hormone T3.

Choline supplementation normalizes plasma glucose in HF-fed Pemt−/− mice

Surprisingly, in Pemt−/− mice fed the HF diet for 2 weeks, the cold-induced weight loss was less than in Pemt+/+ mice, but this difference was completely normalized by choline supplementation (Fig. 8A). However, a 4 h fasting period prior to the cold exposure led to the same amount of weight loss in the four groups of mice (supplementary Fig. 3B). To gain further insight into the mechanism underlying the cold sensitivity of Pemt−/− mice, we investigated the energy supply in plasma after cold exposure of the mice. As shown in Fig. 8B, the amount of NEFAs in plasma of HF-fed Pemt−/− mice was the same as in Pemt+/+ mice, and was not changed by choline supplementation (Fig. 8B). Plasma TG was not different between cold-exposed HF-fed Pemt−/− mice and Pemt+/+ mice (Fig. 8C). The HFCS diet increased the amount of plasma TG in Pemt+/+ mice, but not in Pemt−/− mice (Fig. 8C). However, the level of plasma glucose in HF-fed Pemt−/− mice was 49% lower than in Pemt+/+ mice (Fig. 9A). Although the HFCS diet did not alter plasma glucose levels in Pemt+/+ mice, plasma glucose was increased by 180% in the Pemt−/− mice (Fig. 9A). Thus, these data suggest that reduced plasma glucose, rather than TG or fatty acids, contributes to the cold-induced hypothermia in HF-fed Pemt−/− mice.

An external file that holds a picture, illustration, etc. Object name is 1701fig8.jpg

Choline prevents cold-induced weight loss and normalizes plasma glucose in HF-fed Pemt−/− mice. Pemt+/+ and Pemt−/− mice were fed the HF diet or HFCS diet for 2 weeks, followed by a 4 h fast and cold exposure. A: Cold-induced weight loss in grams (left panel) and as percent of body weight (B.W.) (right panel). B: Plasma NEFAs. C: Plasma TG. All data are from four to seven mice of each group; *P < 0.05 by two-way ANOVA for Pemt+/+ versus Pemt−/−; #P < 0.05 by two-way ANOVA for HF diet versus HFCS diet for the same genotype.

An external file that holds a picture, illustration, etc. Object name is 1701fig9.jpg

Choline normalizes reduced expression of hepatic gluconeogenesis related genes in HF-fed Pemt−/− mice. Pemt+/+ and Pemt−/− mice were fed the HF diet or HFCS diet for 2 weeks, followed by a 4 h fast and cold exposure. A: Plasma glucose. B: Representative immunoblots of PEPCK, PGC1α, protein disulfide isomerase (PDI), and GAPDH. C: Densitometric analysis of amounts of proteins in (B), which were normalized to the amount of GAPDH. All data are from four to seven mice of each group; *P < 0.05 by two-way ANOVA for Pemt+/+ versus Pemt−/−; #P < 0.05 by two-way ANOVA for HF diet versus HFCS diet for the same genotype.

The liver is the major site of glucose production (25). HF-fed Pemt−/− mice show an attenuated hepatic gluconeogenesis and reduced hepatic glycogen (10, 26). To determine whether differences in plasma glucose in Pemt+/+ and Pemt−/− mice were due to hepatic gluconeogenesis, we measured the amounts of a key enzyme in hepatic gluconeogenesis, PEPCK, and its transcription regulator, PGC1α (Fig. 9B, C). Compared with livers of HF-fed Pemt+/+ mice, livers of HF-fed Pemt−/− mice contained 46% less PEPCK protein. Choline supplementation completely restored the amount of this protein to that in HF-fed Pemt+/+ mice. Similarly, the amount of PGC1α in the livers of Pemt−/− mice was 68% lower than in HF-fed Pemt+/+ mice, and the HFCS diet modestly increased the level of PGC1α in Pemt−/− mice. Moreover, the HFCS diet increased the lower level of TG in BAT from Pemt−/− mice to that in Pemt+/+ mice (supplementary Fig. 4). Together, it appears that impaired hepatic gluconeogenesis is associated with cold-induced hypothermia in HF-fed Pemt−/− mice.

DISCUSSION

We report the unexpected finding that HF-fed Pemt−/− mice develop hypothermia upon cold exposure. However, HF-fed Pemt−/− mice show intact thermogenic features in skeletal muscle and in BAT, where PEMT activity is undetectable. Moreover, HF-fed Pemt−/− mice exhibit systolic hypertension and reduced cardiac output. This cold-induced hypothermia, but not the hypertension, in HF-fed Pemt−/− mice is prevented by dietary choline supplementation. Importantly, plasma glucose levels are ~50% lower in HF-fed Pemt−/− mice than in HF-fed Pemt+/+ mice. Choline supplementation normalizes the plasma glucose and raises the expression of hepatic gluconeogenesis-related genes. Together, our results strongly support a link between insufficient plasma glucose supply and cold-induced thermogenesis in HF-fed Pemt−/− mice.

As was the case for mice fed the HF diet for 10 weeks (9), Pemt−/− mice fed the HF diet for only 2 weeks show HF-induced phenotypes: steatohepatitis and resistance to diet-induced adipose hypertrophy (10, 11). Moreover, Pemt−/− mice fed the HF diet for 2 weeks also have elevated heat production and physical activity compared with HF-fed Pemt−/− mice, but only in the dark cycle (Fig. 1A, B). All these data imply that HF-fed Pemt−/− mice expend more energy than Pemt+/+ mice to maintain body temperature under normal housing conditions (at 22–25°C), when thermogenesis is anticipated to be enhanced compared with that of mice housed under thermoneutral conditions at ~30°C (12). The increased energy utilized for thermogenesis at 22–25°C could account for the resistance of HF-fed Pemt−/− mice to DIO. Thus, it is quite surprising that HF-fed Pemt−/− mice become hypothermic upon cold exposure (Fig. 1E). Even though HF-fed Pemt−/− mice contained less WAT mass (11) than HF-fed Pemt+/+ mice, our data indicate that the cold-induced hypothermia of Pemt−/− mice is not due to reduced insulation caused by lower body fat mass. Indeed, chow-fed Pemt−/− mice have the same amount of body mass and contain the same WAT mass as HF-fed Pemt−/− mice (11), but are not cold sensitive (Fig. 2B).

Cold exposure activates the sympathetic nervous system and stimulates energy utilization for thermogenesis, particularly in BAT, to maintain core body temperature (21). Cold-induced sympathetic activity stimulates β-adrenergic receptors on brown adipocytes for promotion of lipolysis for UCP1-mediated thermogenesis. Acute cold exposure also stimulates shivering thermogenesis in skeletal muscle to defend body temperature (12). Maintenance of body temperature upon acute exposure to cold depletes glycogen in the liver and is a high energy-consuming process (27, 28). Thus, cold exposure challenges the cardiovascular system because of the high demand of energy supply for thermogenesis in BAT and skeletal muscle.

To determine the mechanism(s) underlying cold-induced hypothermia in HF-fed Pemt−/− mice, we first explored the possibility of defects in thermogenesis mediated by skeletal muscle and BAT. The thermogenic capacity of skeletal muscle and BAT appeared to be intact in Pemt−/− mice fed the HF diet for 2 weeks, because the rates of fatty acid oxidation and expression of mitochondrial electron transport chain proteins in these tissues were not affected by Pemt genotype. Although both Pemt−/− and Pemt+/+ mice shivered at the beginning of acute cold exposure, Pemt−/− mice showed essentially no shivering after 2 h of cold exposure due to the drop of body temperature. Our data also suggests that the cold-induced hypothermia in HF-fed Pemt−/− mice is unlikely caused by an impaired capacity of thermogenesis in BAT or an inability to mobilize fatty acids from the WAT. The data also suggest that mechanisms other than fatty acid catabolism might cause this unsustainable shivering.

BAT is highly vascularized (29), and cold exposure increases cardiac output and promotes blood flow to BAT (23). We found that systolic blood pressure was higher in HF-fed Pemt−/− mice than in HF-fed Pemt+/+ mice (Fig. 5A), consistent with the observation that hypertensive patients are often sensitive to cold (30). However, it is unlikely that the higher blood pressure in HF-fed Pemt−/− mice accounts for the hypothermia because blood pressure was not decreased by choline supplementation (Fig. 7F). Moreover, choline elevated blood pressure in Pemt+/+ mice (Fig. 7F). However, our data do not completely exclude a possible defect in the cardiovascular systems of HF-fed Pemt−/− mice and a potential connection with cold-induced hypothermia in these mice. On one hand, increased heat loss due to defective peripheral vessel constriction has been linked to cold-sensitivity in thyroid hormone receptor α1 mutant mice (31). On the other hand, HF-fed Pemt−/− mice have choline deficiency (unpublished observations), which might reduce acetylcholine (32), a well-known vascular dilator (33).

BAT consumes not only fatty acids but also large amounts of glucose from blood (34, 35). Cold exposure profoundly increases (by 95-fold) glucose uptake by BAT in rats (36). Chronic infusion of norepinephrine into rats via subcutaneously implanted mini-pumps also markedly increases (by ~50-fold) glucose uptake by BAT (37). Moreover, mice with transplanted BAT have lower body weight, improved glucose tolerance, and increased insulin sensitivity than mice without BAT transplantation, providing strong support for a fundamental role of BAT in global glucose homeostasis (38). In addition, in humans, glucose uptake by BAT is reported to be temperature-sensitive (39, 40), and cold exposure of humans stimulates glucose uptake by BAT (41). Recent studies demonstrate that BAT in humans plays a physiologically significant role in whole-body glucose homeostasis, insulin sensitivity, and energy expenditure (42, 43).

Levels of plasma fatty acids and TG in HF-fed Pemt−/− mice were comparable to those in HF-fed Pemt+/+ mice (Fig. 8B, C), indicating that Pemt−/− mice have an intact capability for mobilization of energy storage and fat supply for thermogenesis. However, the 49% lower level of plasma glucose in Pemt−/− mice compared with Pemt+/+ mice (Fig. 9A) might explain why Pemt−/− mice are hypothermic. When dietary choline supplementation completely reversed the hypothermia in Pemt−/− mice (Fig. 7A), the hypoglycemia and the lower amounts of PEPCK and PGC1α in the livers of Pemt−/− mice were restored and increased respectively (Fig. 9B). These observations are consistent with the finding that PEMT deficiency impairs hepatic gluconeogenesis and reduces hepatic glycogen stores and plasma glucose (10, 26). Choline supplementation increases hepatic gluconeogenesis, plasma glucose, and hepatic glycogen in HF-fed Pemt−/− mice (26). Interestingly, choline supplementation also increases the lower TG content of BAT in HF-fed Pemt−/− mice (supplementary Fig. 4), perhaps reflecting an increased supply of glucose for lipogenesis in the BAT. We conclude, therefore, that compromised hepatic gluconeogenesis and diminished glycogen stores reduce plasma glucose in HF-fed Pemt−/− mice leading to an insufficient supply of energy for thermogenesis upon cold exposure.

In summary, HF-fed Pemt−/− mice become hypothermic upon cold exposure, which is not due to defective thermogenic capacity of skeletal muscle or BAT, or cold stimulated adrenergic signaling. Choline supplementation prevents the hypothermia and plasma hypoglycemia, highlighting the significant contribution of glucose as an energy substrate for thermogenesis during acute cold exposure in HF-fed Pemt−/− mice.

Supplementary Material

Supplemental Data:

Acknowledgments

The authors thank Dr. Gary D. Lopaschuk (University of Alberta) for help with interpretation of cardiac function. They thank Dr. Jerome Yager’s lab (University of Alberta) for technical help with rectal temperature measurements, and Dr. Guergana Tasseva for help with fatty acid oxidation measurements. They thank Susanne Lingrell and Randal Nelson for excellent technical assistance.

Footnotes

Abbreviations:

ATGL
adipose TG lipase
BAT
brown adipose tissue
C/EBPβ
CCAAT/enhancer-binding protein β
DIO
diet-induced obesity
FABP4
fatty acid binding protein 4
HF
high-fat
HFCS
choline-supplemented high-fat
HSL
hormone-sensitive lipase
PC
phosphatidylcholine
PE
phosphatidylethanolamine
PEMT
phosphatidylethanolamine N-methyltransferase
PEPCK
phosphoenolpyruvate carboxykinase
PGC1α
PPARγ coactivator 1α
T3
triiodothyronine
T4
thyroxine
TGH
TG hydrolase
UCP
uncoupling protein
WAT
white adipose tissue

This research was supported by Canadian Institutes of Health Research Grants (MOP 5182 and MOP 133505). R.L.J. is a Canadian Institutes of Health Research New Investigator. X.G. was supported by a studentship from Alberta Innovates Health Solutions. The R.L.J. and D.E.V. laboratories are associated with the Alberta Diabetes Institute and the Alberta Mazankowski Heart Institute.

[S]The online version of this article (available at http://www.jlr.org) contains a supplement.

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