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Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.

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  • 1. Department of Microbiology and Immunology, University of Illinois, College of Medicine, Chicago 60680.
      Authors
    • Nies DH 1
    (1 author)

Proceedings of the National Academy of Sciences of the United States of America, 01 Oct 1989, 86(19):7351-7355
https://doi.org/10.1073/pnas.86.19.7351 PMID: 2678100 PMCID: PMC298059

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Abstract 

Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.

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Proc Natl Acad Sci U S A. 1989 Oct; 86(19): 7351–7355.
PMCID: PMC298059
PMID: 2678100

Expression and nucleotide sequence of a plasmid-determined divalent cation efflux system from Alcaligenes eutrophus.

D H Nies, A Nies, L Chu, and S Silver
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Abstract

Resistance to cobalt, zinc, and cadmium specified by the czc determinant on plasmid pMOL30 in Alcaligenes eutrophus results from a cation efflux system. Five membrane-bound polypeptides that were expressed in Escherichia coli from this determinant under the control of a phage T7 promoter were assigned to four open reading frames identified in the nucleotide sequence of the 6881-base-pair fragment containing the czc putative operon. The contributions of the polypeptides to the cation efflux system were analyzed with deletion derivatives of the 6.9-kilobase fragment, constructed, and expressed in E. coli under the control of the phage T7 promoter and in A. eutrophus under the control of the lac promoter.

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Selected References

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