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Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.

Nucleic Acids Research, 01 Feb 1987, 15(4):1717-1728
https://doi.org/10.1093/nar/15.4.1717 PMID: 3029725 PMCID: PMC340576

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Abstract 

In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.

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Nucleic Acids Res. 1987 Feb 25; 15(4): 1717–1728.
PMCID: PMC340576
PMID: 3029725

Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia.

A Baroin, A Prat, and F Caron
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Abstract

In Paramecium primaurelia, the macronuclear gene encoding the G surface protein is located near a telomere. In this study, multiple copies of this telomere have been isolated and the subtelomeric and telomeric regions of some of them have been sequenced. The telomeric sequences consist of tandem repeats of the hexanucleotides C4A2 or C3A3. We show that the location where these repeats are added, which we call the telomeric site, is variable within a 0.6-0.8-kb region. These results are discussed in relation with the formation of macronuclear DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • McTavish C, Sommerville J. Macronuclear DNA organization and transcription in Paramecium primaurelia. Chromosoma. 1980;78(2):147–164. [Abstract] [Google Scholar]
  • Prescott DM, Murti KG. Chromosome structure in ciliated protozoans. Cold Spring Harb Symp Quant Biol. 1974;38:609–618. [Abstract] [Google Scholar]
  • Gorovsky MA. Genome organization and reorganization in Tetrahymena. Annu Rev Genet. 1980;14:203–239. [Abstract] [Google Scholar]
  • Altschuler MI, Yao MC. Macronuclear DNA of Tetrahymena thermophila exists as defined subchromosomal-sized molecules. Nucleic Acids Res. 1985 Aug 26;13(16):5817–5831. [Europe PMC free article] [Abstract] [Google Scholar]
  • Carle GF, Olson MV. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. [Europe PMC free article] [Abstract] [Google Scholar]
  • Yao MC, Gall JG. A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis. Cell. 1977 Sep;12(1):121–132. [Abstract] [Google Scholar]
  • Pan WC, Blackburn EH. Single extrachromosomal ribosomal RNA gene copies are synthesized during amplification of the rDNA in Tetrahymena. Cell. 1981 Feb;23(2):459–466. [Abstract] [Google Scholar]
  • Karrer KM, Gall JG. The macronuclear ribosomal DNA of Tetrahymena pyriformis is a palindrome. J Mol Biol. 1976 Jun 25;104(2):421–453. [Abstract] [Google Scholar]
  • Engberg J, Andersson P, Leick V, Collins J. Free ribosomal DNA molecules from Tetrahymena pyriformis GL are giant palindromes. J Mol Biol. 1976 Jun 25;104(2):455–470. [Abstract] [Google Scholar]
  • Blackburn EH, Gall JG. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. J Mol Biol. 1978 Mar 25;120(1):33–53. [Abstract] [Google Scholar]
  • King BO, Yao MC. Tandemly repeated hexanucleotide at Tetrahymena rDNA free end is generated from a single copy during development. Cell. 1982 Nov;31(1):177–182. [Abstract] [Google Scholar]
  • Yao MC. Ribosomal RNA gene amplification in Tetrahymena may be associated with chromosome breakage and DNA elimination. Cell. 1981 Jun;24(3):765–774. [Abstract] [Google Scholar]
  • Yao MC, Zhu SG, Yao CH. Gene amplification in Tetrahymena thermophila: formation of extrachromosomal palindromic genes coding for rRNA. Mol Cell Biol. 1985 Jun;5(6):1260–1267. [Europe PMC free article] [Abstract] [Google Scholar]
  • Challoner PB, Blackburn EH. Conservation of sequences adjacent to the telomeric C4A2 repeats of ciliate macronuclear ribosomal RNA gene molecules. Nucleic Acids Res. 1986 Aug 11;14(15):6299–6311. [Europe PMC free article] [Abstract] [Google Scholar]
  • Greider CW, Blackburn EH. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell. 1985 Dec;43(2 Pt 1):405–413. [Abstract] [Google Scholar]
  • Meyer E, Caron F, Baroin A. Macronuclear structure of the G surface antigen gene of Paramecium primaurelia and direct expression of its repeated epitopes in Escherichia coli. Mol Cell Biol. 1985 Sep;5(9):2414–2422. [Europe PMC free article] [Abstract] [Google Scholar]
  • Frischauf AM, Lehrach H, Poustka A, Murray N. Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983 Nov 15;170(4):827–842. [Abstract] [Google Scholar]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [Abstract] [Google Scholar]
  • Prat A, Katinka M, Caron F, Meyer E. Nucleotide sequence of the Paramecium primaurelia G surface protein. A huge protein with a highly periodic structure. J Mol Biol. 1986 May 5;189(1):47–60. [Abstract] [Google Scholar]
  • Yao MC, Yao CH. Repeated hexanucleotide C-C-C-C-A-A is present near free ends of macronuclear DNA of Tetrahymena. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7436–7439. [Europe PMC free article] [Abstract] [Google Scholar]
  • Katzen AL, Cann GM, Blackburn EH. Sequence-specific fragmentation of macronuclear DNA in a holotrichous ciliate. Cell. 1981 May;24(2):313–320. [Abstract] [Google Scholar]
  • Blackburn EH, Budarf ML, Challoner PB, Cherry JM, Howard EA, Katzen AL, Pan WC, Ryan T. DNA termini in ciliate macronuclei. Cold Spring Harb Symp Quant Biol. 1983;47(Pt 2):1195–1207. [Abstract] [Google Scholar]
  • Yokoyama R, Yao MC. Sequence characterization of Tetrahymena macronuclear DNA ends. Nucleic Acids Res. 1986 Mar 11;14(5):2109–2122. [Europe PMC free article] [Abstract] [Google Scholar]
  • Capdeville Y. Allelic modulation in Paramecium aurelia heterozygotes. Study of G serotypes in syngen 1. Mol Gen Genet. 1971;112(4):306–316. [Abstract] [Google Scholar]
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