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Scratch Wound Healing Assay.

Author information

Affiliations

  • 1. DiSIT-Dipartimento di Scienze e Innovazione Tecnologica, University of Piemonte Orientale, Vercelli, Italy.
      Authors
    • Martinotti S 1
    • Ranzato E 1
    (2 authors)

ORCIDs linked to this article

Methods in Molecular Biology (Clifton, N.J.), 01 Jan 2020, 2109:225-229
https://doi.org/10.1007/7651_2019_259 PMID: 31414347 

Abstract 

Cell migration is a crucial step for wound healing. Assays able to evaluate cell migration are very useful to evaluate in vitro wound healing. Scratch wound assay creates a gap in confluent monolayer of keratinocytes to mimic a wound. The protocol of scratch wound is based on few steps: cell culture preparation, scratch wound assay, data acquisition, and data analysis.

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Read article at publisher's site: https://doi.org/10.1007/7651_2019_259

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