Abstract
Free full text
Transformation of yeast.
Abstract
A stable leu2- yeast strain has been transformed to LEU2+ by using a chimeric ColE1 plasmid carrying the yeast leu2 gene. We have used recently developed hybridization and restriction endonuclease mapping techniques to demonstrate directly the presence of the transforming DNA in the yeast genome and also to determine the arrangement of the sequences that were introduced. These studies show that ColE1 DNA together with the yeast sequences can integrate into the yeast chromosomes. This integration may be additive or substitutive. The bacterial plasmid sequences, once integrated, behave as a simple Mendelian element. In addition, we have determined the genetic linkage relationships for each newly introduced LEU2+ allele with the original leu2- allele. These studies show that the transforming squences integrate not only in the leu2 region but also in several other chromosomal locations.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Khan NC, Sen SP. Genetic transformation in yeasts. J Gen Microbiol. 1974 Aug;83(2):237–250. [Abstract] [Google Scholar]
- Mishra NC, Tatum EL. Non-Mendelian inheritance of DNA-induced inositol independence in Neurospora. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3875–3879. [Europe PMC free article] [Abstract] [Google Scholar]
- Ratzkin B, Carbon J. Functional expression of cloned yeast DNA in Escherichia coli. Proc Natl Acad Sci U S A. 1977 Feb;74(2):487–491. [Europe PMC free article] [Abstract] [Google Scholar]
- Hicks J, Fink GR. Identification of chromosomal location of yeast DNA from hybrid plasmid p Yeleu 10. Nature. 1977 Sep 15;269(5625):265–267. [Abstract] [Google Scholar]
- Clewell DB. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol. J Bacteriol. 1972 May;110(2):667–676. [Europe PMC free article] [Abstract] [Google Scholar]
- Guerry P, LeBlanc DJ, Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. [Europe PMC free article] [Abstract] [Google Scholar]
- Cryer DR, Eccleshall R, Marmur J. Isolation of yeast DNA. Methods Cell Biol. 1975;12:39–44. [Abstract] [Google Scholar]
- Hutchison HT, Hartwell LH. Macromolecule synthesis in yeast spheroplasts. J Bacteriol. 1967 Nov;94(5):1697–1705. [Europe PMC free article] [Abstract] [Google Scholar]
- van Solingen P, van der Plaat JB. Fusion of yeast spheroplasts. J Bacteriol. 1977 May;130(2):946–947. [Europe PMC free article] [Abstract] [Google Scholar]
- Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [Abstract] [Google Scholar]
- Maniatis T, Jeffrey A, Kleid DG. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. [Europe PMC free article] [Abstract] [Google Scholar]
- Grunstein M, Hogness DS. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. [Europe PMC free article] [Abstract] [Google Scholar]
- Reanney D. Extrachromosomal elements as possible agents of adaptation and development. Bacteriol Rev. 1976 Sep;40(3):552–590. [Europe PMC free article] [Abstract] [Google Scholar]
Associated Data
Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences
Full text links
Read article at publisher's site: https://doi.org/10.1073/pnas.75.4.1929
Read article for free, from open access legal sources, via Unpaywall:
http://www.pnas.org/content/75/4/1929.full.pdf
Citations & impact
Impact metrics
Citations of article over time
Alternative metrics
Article citations
Four decades of Eukaryotic DNA replication: From yeast genetics to high-resolution cryo-EM structures of the replisome.
Proc Natl Acad Sci U S A, 121(42):e2415231121, 04 Oct 2024
Cited by: 0 articles | PMID: 39365830
A replisome-associated histone H3-H4 chaperone required for epigenetic inheritance.
Cell, 187(18):5010-5028.e24, 01 Aug 2024
Cited by: 0 articles | PMID: 39094570 | PMCID: PMC11380579
Advancing Cordyceps militaris Industry: Gene Manipulation and Sustainable Biotechnological Strategies.
Bioengineering (Basel), 11(8):783, 02 Aug 2024
Cited by: 0 articles | PMID: 39199741 | PMCID: PMC11351413
Review
Free full text in Europe PMCWhat are the 100 most cited fungal genera?
Stud Mycol, 108:1-411, 15 Jul 2024
Cited by: 1 article | PMID: 39100921 | PMCID: PMC11293126
Conversion of polyploid and alloploid Saccharomyces sensu stricto strains to leu2 mutants by genome DNA editing.
Appl Microbiol Biotechnol, 108(1):416, 12 Jul 2024
Cited by: 0 articles | PMID: 38995331 | PMCID: PMC11245423
Go to all (946) article citations
Other citations
Data
Similar Articles
To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.
Transformation of yeast by a replicating hybrid plasmid.
Nature, 275(5676):104-109, 01 Sep 1978
Cited by: 787 articles | PMID: 357984
Functional expression in yeast of the Escherichia coli plasmid gene coding for chloramphenicol acetyltransferase.
Proc Natl Acad Sci U S A, 77(2):1078-1082, 01 Feb 1980
Cited by: 24 articles | PMID: 6987664 | PMCID: PMC348427
[Insertion of transposon Tn9 into the spinal (Escherichia coli-Saccharomyces cerevisiae) plasmids and the expression of the prokaryotic gene of chloramphenicol resistance in yeast cells].
Genetika, 19(4):541-547, 01 Apr 1983
Cited by: 0 articles | PMID: 6305766
Plasmid transformation of Escherichia coli and other bacteria.
Methods Enzymol, 204:63-113, 01 Jan 1991
Cited by: 332 articles | PMID: 1943786
Review
