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Heterogeneity of group A streptococcal pyrogenic exotoxin type B.

Infection and Immunity, 01 May 1978, 20(2):512-518
https://doi.org/10.1128/iai.20.2.512-518.1978 PMID: 352946 PMCID: PMC421884

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Abstract 

Streptococcal pyrogenic exotoxin type B purified from culture filtrates of either the NY-5 or T-19 strain of group A streptococcus was found to be heterogeneous in charge. Three protein fractions with isoelectric points of 8.0, 8.4, and 9.0 were isolated by differential solubility in ethanol and acetate-buffered saline followed by isoelectric focusing and shown to be antigenically identical to streptococcal pyrogenic exotoxin type B. The molecular weights of all three fractions were approximately 17,500, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with aggregates forming in the presence of hyaluronic acid. Only the pI 8.4 fraction showed the characteristic activities of streptococcal pyrogenic exotoxin in rabbits: pyrogenicity and ability to enhance susceptibility to lethal endotoxin shock. The pI 8.0 and pI 9.0 fractions were not pyrogenic, but could be used to immunize against pyrogenicity. These two fractions failed either to enhance lethal endotoxin shock or to immunize against enhancement activity. When the isolated fractions were electrofocused again they appeared heterogeneous, suggesting an instability of the B toxin molecular forms.

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Infect Immun. 1978 May; 20(2): 512–518.
PMCID: PMC421884
PMID: 352946

Heterogeneity of group A streptococcal pyrogenic exotoxin type B.

E L Barsumian, C M Cunningham, P M Schlievert, and D W Watson
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Abstract

Streptococcal pyrogenic exotoxin type B purified from culture filtrates of either the NY-5 or T-19 strain of group A streptococcus was found to be heterogeneous in charge. Three protein fractions with isoelectric points of 8.0, 8.4, and 9.0 were isolated by differential solubility in ethanol and acetate-buffered saline followed by isoelectric focusing and shown to be antigenically identical to streptococcal pyrogenic exotoxin type B. The molecular weights of all three fractions were approximately 17,500, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, with aggregates forming in the presence of hyaluronic acid. Only the pI 8.4 fraction showed the characteristic activities of streptococcal pyrogenic exotoxin in rabbits: pyrogenicity and ability to enhance susceptibility to lethal endotoxin shock. The pI 8.0 and pI 9.0 fractions were not pyrogenic, but could be used to immunize against pyrogenicity. These two fractions failed either to enhance lethal endotoxin shock or to immunize against enhancement activity. When the isolated fractions were electrofocused again they appeared heterogeneous, suggesting an instability of the B toxin molecular forms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Awdeh ZL, Williamson AR, Askonas BA. One cell-one immunoglobulin. Origin of limited heterogeneity of myeloma proteins. Biochem J. 1970 Jan;116(2):241–248. [Europe PMC free article] [Abstract] [Google Scholar]
  • BAIRD-PARKER AC, JOSEPH RL. FRACTIONATION OF STAPHYLOCOCCAL ENTEROTOXIN B. Nature. 1964 May 9;202:570–571. [Abstract] [Google Scholar]
  • Borja CR, Bergdoll MS. Purification and partial characterization of enterotoxin C produced by Staphylococcus aureus strain 137. Biochemistry. 1967 May;6(5):1467–1473. [Abstract] [Google Scholar]
  • Chu FS, Thadhani K, Schantz EJ, Bergdoll MS. Purification and characterization of staphylococcal enterotoxin A. Biochemistry. 1966 Oct;5(10):3281–3289. [Abstract] [Google Scholar]
  • Cunningham CM, Barsumian EL, Watson DW. Further purification of group A streptococcal pyrogenic exotoxin and characterization of the purified toxin. Infect Immun. 1976 Sep;14(3):767–775. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cunningham CM, Watson DW. Alteration of clearance function by group A streptococcal pyrogenic exotoxin and its relation to suppression of the antibody response. Infect Immun. 1978 Jan;19(1):51–57. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cunningham CM, Watson DW. Suppression of antibody response by group A streptococcal pyrogenic exotoxin and characterization of the cells involved. Infect Immun. 1978 Feb;19(2):470–476. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hanna EE, Hale M. Deregulation of mouse antibody-forming cells in vivo in cell culture by streptococcal pyrogenic exotoxin. Infect Immun. 1975 Feb;11(2):265–272. [Europe PMC free article] [Abstract] [Google Scholar]
  • HANNA EE, WATSON DW. HOST-PARASITE RELATIONSHIPS AMONG GROUP A STREPTOCOCCI. 3. DEPRESSION OF RETICULOENDOTHELIAL FUNCTION BY STREPTOCOCCAL PYROGENIC EXOTOXINS. J Bacteriol. 1965 Jan;89:154–158. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hanna EE, Watson DW. Host-parasite relationships among group A streptococci. IV. Suppression of antibody response by streptococcal pyrogenic exotoxin. J Bacteriol. 1968 Jan;95(1):14–21. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hanna EE, Watson DW. Enhanced immune response after immunosuppression by Streptococcal pyrogenic exotoxin. Infect Immun. 1973 Jun;7(6):1009–1011. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hayes MB, Wellner D. Microheterogeneity of L-amino acid oxidase. Separation of multiple components by polyacrylamide gel electrofucusing. J Biol Chem. 1969 Dec 25;244(24):6636–6644. [Abstract] [Google Scholar]
  • Kim YB, Watson DW. A purified group A streptococcal pyrogenic exotoxin. Physiochemical and biological properties including the enhancement of susceptibility to endotoxin lethal shock. J Exp Med. 1970 Mar 1;131(3):611–622. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kondo I, Sakurai S, Sarai Y. Purification of exfoliatin produced by Staphylococcus aureus of bacteriophage group 2 and its physicochemical properties. Infect Immun. 1973 Aug;8(2):156–164. [Europe PMC free article] [Abstract] [Google Scholar]
  • Nauciel C, Blass J, Mangalo R, Raynaud M. Evidence for two molecular forms of streptococcal erythrogenic toxin. Conversion to a single form by 2-mercaptoethanol. Eur J Biochem. 1969 Nov;11(1):160–164. [Abstract] [Google Scholar]
  • Schlievert PM, Bettin KM, Watson DW. Purification and characterization of group A streptococcal pyrogenic exotoxin type C. Infect Immun. 1977 May;16(2):673–679. [Europe PMC free article] [Abstract] [Google Scholar]
  • Schuh V, Hríbalová V, Atkins E. The pyrogenic effect of scarlet fever toxin. IV. Pyrogenicity of strain C 203 U filtrate: comparison with some basic characteristics of the known types of scarlet fever toxin. Yale J Biol Med. 1970 Aug;43(1):31–42. [Europe PMC free article] [Abstract] [Google Scholar]
  • SCHWAB JH, WATSON DW, CROMARTIE WJ. Production of generalized Shwartzman reaction with group A streptococcal factors. Proc Soc Exp Biol Med. 1953 Apr;82(4):754–761. [Abstract] [Google Scholar]
  • SCHWAB JH, WATSON DW, CROMARTIE WJ. Further studies of group A streptococcal factors with lethal and cardiotoxic properties. J Infect Dis. 1955 Jan-Feb;96(1):14–18. [Abstract] [Google Scholar]
  • WATSON DW. Host-parasite factors in group A streptococcal infections. Pyrogenic and other effects of immunologic distinct exotoxins related to scarlet fever toxins. J Exp Med. 1960 Feb 1;111:255–284. [Europe PMC free article] [Abstract] [Google Scholar]
  • Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [Abstract] [Google Scholar]
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