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Somatic mutation and clonal expansion of B cells in an antigen-driven immune response.

The EMBO Journal, 01 Feb 1985, 4(2):345-350
https://doi.org/10.1002/j.1460-2075.1985.tb03635.x PMID: 3926481 PMCID: PMC554192

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Abstract 

The variable (V) regions of three closely related monoclonal antibodies produced by hybridomas which had been isolated from a single mouse were sequenced at the level of the mRNA. The sequences and the restriction analysis of the immunoglobulin loci carried by the hybridoma cells indicate that the antibodies are derived from cells belonging to a single B cell clone. The sequence data imply a high frequency and stepwise occurrence of somatic point mutations in the expressed V region genes and substantial clonal expansion of B cells in the mouse. The mutations appear to be randomly introduced into heavy and light chain V region genes. Mutations are also seen in the complementarity determining regions which may thus have been involved in the selection of the cells producing the three antibodies.

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EMBO J. 1985 Feb; 4(2): 345–350.
PMCID: PMC554192
PMID: 3926481

Somatic mutation and clonal expansion of B cells in an antigen-driven immune response.

F Sablitzky, G Wildner, and K Rajewsky
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Abstract

The variable (V) regions of three closely related monoclonal antibodies produced by hybridomas which had been isolated from a single mouse were sequenced at the level of the mRNA. The sequences and the restriction analysis of the immunoglobulin loci carried by the hybridoma cells indicate that the antibodies are derived from cells belonging to a single B cell clone. The sequence data imply a high frequency and stepwise occurrence of somatic point mutations in the expressed V region genes and substantial clonal expansion of B cells in the mouse. The mutations appear to be randomly introduced into heavy and light chain V region genes. Mutations are also seen in the complementarity determining regions which may thus have been involved in the selection of the cells producing the three antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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