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Requirement for membrane potential in injection of phage T4 DNA.

Proceedings of the National Academy of Sciences of the United States of America, 01 Sep 1979, 76(9):4669-4673
https://doi.org/10.1073/pnas.76.9.4669 PMID: 41245 PMCID: PMC411642

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Abstract 

The first stages of infection by phage T4 may be divided into energy-dependent and energy-independent processes. Irreversible adsorption, unplugging, and initial exposure of the DNA terminus may occur at 4 degrees C, or at 37 degrees C in bacteria whose energy-yielding metabolism has been poisoned. DNA injection into the cytoplasm needs higher temperatures and energy from the host cell. The nature of this energy requirements was deduced from the use of metabolic inhibitors. Our results show that T4 DNA injection specifically requires the presence of a protonmotive force across the cytoplasmic membrane of the host. Moreover, the chemical gradient (delta pH) does not appear to be essential, but the membrane potential (delta psi) is required.

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Proc Natl Acad Sci U S A. 1979 Sep; 76(9): 4669–4673.
PMCID: PMC411642
PMID: 41245

Requirement for membrane potential in injection of phage T4 DNA.

B Labedan and E B Goldberg
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Abstract

The first stages of infection by phage T4 may be divided into energy-dependent and energy-independent processes. Irreversible adsorption, unplugging, and initial exposure of the DNA terminus may occur at 4 degrees C, or at 37 degrees C in bacteria whose energy-yielding metabolism has been poisoned. DNA injection into the cytoplasm needs higher temperatures and energy from the host cell. The nature of this energy requirements was deduced from the use of metabolic inhibitors. Our results show that T4 DNA injection specifically requires the presence of a protonmotive force across the cytoplasmic membrane of the host. Moreover, the chemical gradient (delta pH) does not appear to be essential, but the membrane potential (delta psi) is required.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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