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The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.

The Journal of Experimental Medicine, 01 Nov 1969, 130(5):1145-1160
https://doi.org/10.1084/jem.130.5.1145 PMID: 5347695 PMCID: PMC2180480

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Abstract 

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.

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J Exp Med. 1969 Oct 31; 130(5): 1145–1160.
PMCID: PMC2180480
PMID: 5347695

THE EIGHTH COMPONENT OF HUMAN COMPLEMENT (C8): ISOLATION, CHARACTERIZATION, AND HEMOLYTIC EFFICIENCY

Jorge A. Manni and Hans J. Müller-Eberhard
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Abstract

A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a γ1-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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