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Micronuclear RNA synthesis in Paramecium caudatum.

The Journal of Cell Biology, 01 May 1967, 33(2):281-285
https://doi.org/10.1083/jcb.33.2.281 PMID: 6039372 PMCID: PMC2108365

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Abstract 

In a generation time of 8 hr in Paramecium caudatum, the bulk of DNA synthesis detected by thymidine-(3)H incorporation takes place in the latter part of the cell cycle. The micronuclear cycle includes a G(1) of 3 hr followed by an S period of 3-3(1/2) hr. G(2) and division occupies the remaining period of the cycle. Macronuclear RNA synthesis detected by 5'-uridine-(3)H incorporation is continuous throughout the cell cycle. Micronuclear RNA synthesis is restricted to the S period. Ribonuclease removes 80-90% of the incorporated label. Pulse-chase experiments showed that part of the RNA is conserved and released to the cytoplasm during the succeeding G(1) period.

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J Cell Biol. 1967 May 1; 33(2): 281–285.
PMCID: PMC2108365
PMID: 6039372

MICRONUCLEAR RNA SYNTHESIS IN PARAMECIUM CAUDATUM

M. V. Narasimha Rao and David M. Prescott
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Abstract

In a generation time of 8 hr in Paramecium caudatum, the bulk of DNA synthesis detected by thymidine-3H incorporation takes place in the latter part of the cell cycle. The micronuclear cycle includes a G1 of 3 hr followed by an S period of 3–3½ hr. G2 and division occupies the remaining period of the cycle. Macronuclear RNA synthesis detected by 5'-uridine-3H incorporation is continuous throughout the cell cycle. Micronuclear RNA synthesis is restricted to the S period. Ribonuclease removes 80–90% of the incorporated label. Pulse-chase experiments showed that part of the RNA is conserved and released to the cytoplasm during the succeeding G1 period.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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