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Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.

Proceedings of the National Academy of Sciences of the United States of America, 01 Aug 1981, 78(8):4887-4891
https://doi.org/10.1073/pnas.78.8.4887 PMID: 6272282 PMCID: PMC320284

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Abstract 

The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kilobases and represented all but the 5' 115 bases of poliovirus RNA. A third clone was generated from primer-extended DNA and contained sequences from the 5' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5' end of the RNA and extending to a termination codon 71 bases from the 3' end contained known poliovirus polypeptide sequence.

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Proc Natl Acad Sci U S A. 1981 Aug; 78(8): 4887–4891.
PMCID: PMC320284
PMID: 6272282

Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.

V R Racaniello and D Baltimore
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Abstract

The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kilobases and represented all but the 5' 115 bases of poliovirus RNA. A third clone was generated from primer-extended DNA and contained sequences from the 5' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5' end of the RNA and extending to a termination codon 71 bases from the 3' end contained known poliovirus polypeptide sequence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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