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Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

Molecular and Cellular Biology, 01 Sep 1983, 3(9):1562-1566
https://doi.org/10.1128/mcb.3.9.1562-1566.1983 PMID: 6415403 PMCID: PMC370009

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Abstract 

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.

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Mol Cell Biol. 1983 Sep; 3(9): 1562–1566.
PMCID: PMC370009
PMID: 6415403

Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.

K G Murti and D M Prescott
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Abstract

Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed: (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that: (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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