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A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor.

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Affiliations

  • 1. Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.
      Authors
    • Gepner J 1
    (1 author)

Proceedings of the National Academy of Sciences of the United States of America, 01 Dec 1993, 90(23):11132-11136
https://doi.org/10.1073/pnas.90.23.11132 PMID: 8248219 PMCID: PMC47936

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A comment on this article appears in "Conventional protein coding genes in the Drosophila Y chromosome: is the puzzle of the fertility gene function solved?" Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):10904-6. This is a comment on "What determines where alpha-helices begin and end?" Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):10907-8.

Abstract 

A clone encoding a portion of the highly conserved ATP-binding domain of a dynein heavy-chain polypeptide was mapped to a region of the Drosophila melanogaster Y chromosome. Dyneins are large multisubunit enzymes that utilize the hydrolysis of ATP to move along microtubules. They were first identified as the motors that provide the force for flagellar and ciliary bending. Seven different dynein heavy-chain genes have been identified in D. melanogaster by PCR. In the present study, we demonstrate that one of the dynein genes, Dhc-Yh3, is located in Y chromosome region h3, which is contained within kl-5, a locus required for male fertility. The PCR clone derived from Dhc-Yh3 is 85% identical to the corresponding region of the beta heavy chain of sea urchin flagellar dynein but only 53% identical to a cytoplasmic dynein heavy chain from Drosophila. In situ hybridization to Drosophila testes shows Dhc-Yh3 is expressed in wild-type males but not in males missing the kl-5 region. These results are consistent with the hypothesis that the Y chromosome is needed for male fertility because it contains conventional genes that function during spermiogenesis.

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Proc Natl Acad Sci U S A. 1993 Dec 1; 90(23): 11132–11136.
PMCID: PMC47936
PMID: 8248219

A fertility region on the Y chromosome of Drosophila melanogaster encodes a dynein microtubule motor.

J Gepner and T S Hays
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Abstract

A clone encoding a portion of the highly conserved ATP-binding domain of a dynein heavy-chain polypeptide was mapped to a region of the Drosophila melanogaster Y chromosome. Dyneins are large multisubunit enzymes that utilize the hydrolysis of ATP to move along microtubules. They were first identified as the motors that provide the force for flagellar and ciliary bending. Seven different dynein heavy-chain genes have been identified in D. melanogaster by PCR. In the present study, we demonstrate that one of the dynein genes, Dhc-Yh3, is located in Y chromosome region h3, which is contained within kl-5, a locus required for male fertility. The PCR clone derived from Dhc-Yh3 is 85% identical to the corresponding region of the beta heavy chain of sea urchin flagellar dynein but only 53% identical to a cytoplasmic dynein heavy chain from Drosophila. In situ hybridization to Drosophila testes shows Dhc-Yh3 is expressed in wild-type males but not in males missing the kl-5 region. These results are consistent with the hypothesis that the Y chromosome is needed for male fertility because it contains conventional genes that function during spermiogenesis.

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Selected References

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