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Recombination-mediated PCR-directed plasmid construction in vivo in yeast.

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  • 1. Affymax Research Institute, 4001 Miranda Avenue, Palo Alto, CA 94304, USA.
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    • Oldenburg KR 1
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Nucleic Acids Research, 01 Jan 1997, 25(2):451-452
https://doi.org/10.1093/nar/25.2.451 PMID: 9016579 PMCID: PMC146432

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Abstract 

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.

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Nucleic Acids Res. 1997 Jan 15; 25(2): 451–452.
PMCID: PMC146432
PMID: 9016579

Recombination-mediated PCR-directed plasmid construction in vivo in yeast.

K R Oldenburg, K T Vo, S Michaelis, and C Paddon
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Abstract

We have extended the technique of PCR-directed recombination in Saccharomyces cerevisiae to develop a simple method for plasmid or gene construction in the absence of suitable restriction sites. The DNA to be cloned is PCR-amplified with 30-40 bp of homology to a linearized yeast plasmid. Co-transformation into yeast results in homologous recombination at a position directed by the PCR oligonucleotides.

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Selected References

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NIGMS NIH HHS (2)