Figure 5

Generation and validation of a MET^−/− pancreatic carcinoma cell line. (a) Flow cytometry analysis of MET expression at the surface of wild-type (wt) and MET^−/− Capan-I cells. (b) HGF-dependent phosphorylation of MET and activation of downstream transducers in lysates from wild-type (wt) and MET^−/− Capan-I cells. Vinculin was used as a loading control. p145 MET: MET receptor β chain; p145 P-MET: phosphorylated MET receptor β chain; p190 MET: precursor form of the MET receptor; p60AKT: AKT; p60 P-AKT: phosphorylated AKT; p44/42ERK: ERK; p44/42 P-ERK: phosphorylated ERK; p117 Vinculin: vinculin. The uncropped Western blots are shown in Supplementary Materials File S1.