Figure 2

Schematic overview of gene mutations in plasma (P) and pancreatic juice (PJ) for 1 patient with high-grade dysplasia (HGD; #1) and 25 patients with pancreatic cancer (PC; #2–#26). Only genes showing an alteration are presented. Gene mutations in plasma and PJ #1–4 were evaluated using the Oncomine colon cfDNA assay, and for #5–26, the Accel-Amplicon 57G Plus pan-cancer profiling panel was used. For the tissue (#1–4, #6) and biopsy samples (#5), an in-house-generated pan-cancer panel was used. Concordance of KRAS between PJ and plasma was seen in three patients (#6, #16, and #17), whereas concordance of TP53 between PJ and plasma was seen in two patients (#13 and #20). Furthermore, patients with a P72R heterozygous variant had a higher TP53 detection rate than those with a homozygous variant. HGD = high-grade dysplasia, PC = pancreatic cancer, Tis = tissue, P = plasma, PJ = pancreatic juice, and AF = allelic frequency. * indicates a nonsense mutation.