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FIG. 1.

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Analysis of recognition patterns of anti-P. falciparum antisera by Western blotting. Saponin-lysed mature 3D7 schizonts were probed with rabbit polyclonal antisera raised against EBA175 (lane 1) and PfAMA1 ectodomain (strain 3D7) (lane 3). The reactivities of mouse MAbs 5G8 (lane 2) against the AMA1 pro-region and 1F9 (lane 4) against AMA1 domain 1 are also shown. The parasite samples in lanes 1 and 2 were treated with 2-mercaptoethanol as a reducing agent, whereas those in lanes 3 and 4 were run under nonreducing conditions, as the epitope for MAb 1F9 has been shown to be reduction sensitive.

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