Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin.

The Journal of Experimental Medicine, 01 May 1959, 109(5):475-485
https://doi.org/10.1084/jem.109.5.475 PMID: 13641571 PMCID: PMC2136976

This article is in the Europe PMC Open access subset. Refer to the copyright information in the article for licensing details.
Free full text in Europe PMC

Abstract 

Primary or established strain cultures of a variety of primate cells that were susceptible to cytopathic infection strongly adsorbed poliovirus. Insusceptible non-primate cells in primary or established-strain culture did not so adsorb virus (or propagate it), with exception of the ERK-1 embryo rabbit kidney strain. All tested cells, regardless of type or susceptibility, adsorbed about 1 per cent of input virus, which became cell-associated (CAV) without loss of infectivity. In combination with susceptible or insusceptible cells, CAV was only about 90 per cent neutralized by homotypic antiserum. CAV eluted continuously from non-susceptible cells with continued incubation; eluted virus gave rise to infection and new CAV to the same degree in susceptible cells as did original virus.

Free full text 

Logo of jexpmedLink to Publisher's site
J Exp Med. 1959 May 1; 109(5): 475–485.
PMCID: PMC2136976
PMID: 13641571

THE MAMMALIAN CELL-VIRUS RELATIONSHIP

I. ATTACHMENT OF POLIOVIRUS TO CULTIVATED CELLS OF PRIMATE AND NON-PRIMATE ORIGIN
Leroy C. McLaren, John J. Holland, and Jerome T. Syverton
Author information Article notes Copyright and License information Disclaimer

Abstract

Primary or established strain cultures of a variety of primate cells that were susceptible to cytopathic infection strongly adsorbed poliovirus. Insusceptible non-primate cells in primary or established-strain culture did not so adsorb virus (or propagate it), with exception of the ERK-1 embryo rabbit kidney strain. All tested cells, regardless of type or susceptibility, adsorbed about 1 per cent of input virus, which became cell-associated (CAV) without loss of infectivity. In combination with susceptible or insusceptible cells, CAV was only about 90 per cent neutralized by homotypic antiserum. CAV eluted continuously from non-susceptible cells with continued incubation; eluted virus gave rise to infection and new CAV to the same degree in susceptible cells as did original virus.

Full Text

The Full Text of this article is available as a PDF (549K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • SYVERTON JT, McLAREN LC. Human cells in continuous culture. I. Derivation of cell strains from esophagus, palate, liver, and lung. Cancer Res. 1957 Oct;17(9):923–926. [Abstract] [Google Scholar]
  • SCHERER WF, SYVERTON JT, GEY GO. Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix. J Exp Med. 1953 May;97(5):695–710. [Europe PMC free article] [Abstract] [Google Scholar]
  • PULVERTAFT RJ, DAVIES JR, WEISS L, WILKINSON JH. Studies on tissue cultures of human pathological thyroids. J Pathol Bacteriol. 1959 Jan;77(1):19–32. [Abstract] [Google Scholar]
  • BERMAN L, STULBERG CS. Eight culture strains (Detroit) of human epithelial-like cells. Proc Soc Exp Biol Med. 1956 Aug-Sep;92(4):730–735. [Abstract] [Google Scholar]
  • WESTWOOD JC, MACPHERSON IA, TITMUSS DH. Transformation of normal cells in tissue culture: its significance relative to malignancy and virus vaccine production. Br J Exp Pathol. 1957 Apr;38(2):138–154. [Europe PMC free article] [Abstract] [Google Scholar]
  • PUCK TT, MARCUS PI, CIECIURA SJ. Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer. J Exp Med. 1956 Feb 1;103(2):273–283. [Europe PMC free article] [Abstract] [Google Scholar]
  • GIFFORD GE, SYVERTON JT. Replication of poliovirus in primate cell cultures maintained under anaerobic conditions. Virology. 1957 Oct;4(2):216–223. [Abstract] [Google Scholar]
  • SANFORD KK, EARLE WR, LIKELY GD. The growth in vitro of single isolated tissue cells. J Natl Cancer Inst. 1948 Dec;9(3):229–246. [Abstract] [Google Scholar]
  • RUBIN H, FRANKLIN RM, BALUDA M. Infection and growth of Newcastle disease virus (NDV) in cultures of chick embryo lung epithelium. Virology. 1957 Jun;3(3):587–600. [Abstract] [Google Scholar]
  • KAPLAN AS. A study of the herpes simplex virus-rabbit kidney cell system by the plaque technique. Virology. 1957 Dec;4(3):435–457. [Abstract] [Google Scholar]
  • KAPLAN AS, MELNICK JL. Multiplication of virulent poliovirus in capuchin monkey kidney cultures without microscopically observed cytopathogenicity. Proc Soc Exp Biol Med. 1955 Dec;90(3):562–565. [Abstract] [Google Scholar]
  • DARNELL JE., Jr Adsorption and maturation of poliovirus in singly and multiply infected HeLa cells. J Exp Med. 1958 May 1;107(5):633–641. [Europe PMC free article] [Abstract] [Google Scholar]
  • ACKERMANN WW, ISHIDA N, MAASSAB HF. Growth characteristics of influenza virus concerning the binding of virus by host cells. J Exp Med. 1955 Nov 1;102(5):545–554. [Europe PMC free article] [Abstract] [Google Scholar]
  • WARREN J, CUTCHINS EC. General characteristics and viral susceptibility of bovine embryonic tissue cultures. Virology. 1957 Oct;4(2):297–304. [Abstract] [Google Scholar]
  • BACHRACH HL, CALLIS JJ, HESS WR, PATTY RE. A plaque assay for foot-and-mouth disease virus and kinetics of virus reproduction. Virology. 1957 Oct;4(2):224–236. [Abstract] [Google Scholar]
  • YOUNGNER JS. Virus adsorption and plaque formation in monolayer cultures of trypsin-dispersed monkey kidney. J Immunol. 1956 Apr;76(4):288–292. [Abstract] [Google Scholar]
  • HSIUNG GD, MELNICK JL. Adsorption, multiplication, and cytopathogenicity of enteroviruses (poliomyelitis, Coxsackie, and ECHO groups) in susceptible and resistant monkey kidney cells. J Immunol. 1958 Jan;80(1):45–52. [Abstract] [Google Scholar]
  • DREW RM. Isolation and propagation of rabbit kidney epithelialike cells. Science. 1957 Oct 18;126(3277):747–748. [Abstract] [Google Scholar]
  • DUNHAM WB, EWING FM. Propagation of poliovirus in chick embryo cell cultures. I. Cultivation of 3 virus types. Proc Soc Exp Biol Med. 1957 Aug-Sep;95(4):637–639. [Abstract] [Google Scholar]
  • HOLLAND JJ, McLAREN LC. The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of poliovirus by HeLa cells. J Exp Med. 1959 May 1;109(5):487–504. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Full text links 

Read article at publisher's site: https://doi.org/10.1084/jem.109.5.475

Read article for free, from open access legal sources, via Unpaywall: http://jem.rupress.org/content/109/5/475.full.pdfUnpaywall

Citations & impact 

Impact metrics

111
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/57638084
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/57638084

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1084/jem.109.5.475

Supporting
Mentioning
Contrasting
0
21
0

Article citations

Go to all (111) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.