Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

The membrane attack mechanism of complement: the three polypeptide chain structure of the eigth component (C8).

The Journal of Experimental Medicine, 01 May 1976, 143(5):1131-1139
https://doi.org/10.1084/jem.143.5.1131 PMID: 1262784 PMCID: PMC2190193

This article is in the Europe PMC Open access subset. Refer to the copyright information in the article for licensing details.
Free full text in Europe PMC

Abstract 

The purification of human C8 in milligram quantities from outdated human serum was achieved by ammonium sulfate precipitation (37.5-50% saturation) and ion exchange column chromatography employing CM-32 cellulose and QAE-Sephadex. The yield of C8 activity ranged from 2-9%, and the average purification was 1,700-fold. Fully reduced C8 was shown by SDS polyacrylamide gel electrophoresis to have three polypeptide chains which were present in equimolor ratios: alpha, 77,000 daltons; beta, 63,000 daltons; and gamma, 13,700 daltons. C8 denaturation by SDS and urea in the absence of reducing agents revealed two noncovalently linked subunits: alpha-gamma, 99,000 daltons, and beta, 75,000 daltons.

Free full text 

Logo of jexpmedLink to Publisher's site
J Exp Med. 1976 May 1; 143(5): 1131–1139.
PMCID: PMC2190193
PMID: 1262784

The membrane attack mechanism of complement: the three polypeptide chain structure of the eigth component (C8)

Copyright and License information Disclaimer

Abstract

The purification of human C8 in milligram quantities from outdated human serum was achieved by ammonium sulfate precipitation (37.5-50% saturation) and ion exchange column chromatography employing CM-32 cellulose and QAE-Sephadex. The yield of C8 activity ranged from 2-9%, and the average purification was 1,700-fold. Fully reduced C8 was shown by SDS polyacrylamide gel electrophoresis to have three polypeptide chains which were present in equimolor ratios: alpha, 77,000 daltons; beta, 63,000 daltons; and gamma, 13,700 daltons. C8 denaturation by SDS and urea in the absence of reducing agents revealed two noncovalently linked subunits: alpha-gamma, 99,000 daltons, and beta, 75,000 daltons.

Full Text

The Full Text of this article is available as a PDF (674K).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Stolfi RL. Immune lytic transformation: a state of irreversible damage generated as a result of the reaction of the eighth component in the guinea pig complement system. J Immunol. 1968 Jan;100(1):46–54. [Abstract] [Google Scholar]
  • Hadding U, Müller-Eberhard HJ. Complement: substitution of the terminal component in immune hemolysis by 1,10-phenanthroline. Science. 1967 Jul 28;157(3787):442–443. [Abstract] [Google Scholar]
  • Stolfi RL. An analogue of guinea pig C8: in vitro generation and inhibitory activity. J Immunol. 1970 May;104(5):1212–1219. [Abstract] [Google Scholar]
  • Tamura N, Shimada A, Chang S. Further evidence for immune cytolysis by antibody and the first eight components of complement in the absence of C9. Immunology. 1972 Jan;22(1):131–140. [Abstract] [Google Scholar]
  • Kolb WP, Haxby JA, Arroyave CM, Müller-Eberhard HJ. Molecular analysis of the membrane attack mechanism of complement. J Exp Med. 1972 Mar 1;135(3):549–566. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kolb WP, Müller-Eberhard HJ. The membrane attack mechanism of complement. Verification of a stable C5-9 complex in free solution. J Exp Med. 1973 Aug 1;138(2):438–451. [Europe PMC free article] [Abstract] [Google Scholar]
  • Kolb WP, Muller-Eberhard HJ. The membrane attack mechanism of complement. Isolation and subunit composition of the C5b-9 complex. J Exp Med. 1975 Apr 1;141(4):724–735. [Europe PMC free article] [Abstract] [Google Scholar]
  • Hadding U, Müller-Eberhard HJ. The ninth component of human complement: isolation, description and mode of action. Immunology. 1969 Jun;16(6):719–735. [Abstract] [Google Scholar]
  • McConahey PJ, Dixon FJ. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. [Abstract] [Google Scholar]
  • Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [Abstract] [Google Scholar]
  • Glossmann H, Neville DM., Jr Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat. J Biol Chem. 1971 Oct 25;246(20):6339–6346. [Abstract] [Google Scholar]
  • Müller-Eberhard HJ. Complement. Annu Rev Biochem. 1975;44:697–724. [Abstract] [Google Scholar]
  • Fish WW, Reynolds JA, Tanford C. Gel chromatography of proteins in denaturing solvents. Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants. J Biol Chem. 1970 Oct 10;245(19):5166–5168. [Abstract] [Google Scholar]
  • Manni JA, Müller-Eberhard HJ. The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency. J Exp Med. 1969 Nov 1;130(5):1145–1160. [Europe PMC free article] [Abstract] [Google Scholar]
  • Heusser C, Boesman M, Nordin JH, Isliker H. Effect of chemical and enzymatic radioiodination on in vitro human Clq activities. J Immunol. 1973 Mar;110(3):820–828. [Abstract] [Google Scholar]
Articles from The Journal of Experimental Medicine are provided here courtesy of The Rockefeller University Press

Full text links 

Read article at publisher's site: https://doi.org/10.1084/jem.143.5.1131

Read article for free, from open access legal sources, via Unpaywall: https://rupress.org/jem/article-pdf/143/5/1131/1087698/1131.pdfUnpaywall

Citations & impact 

Impact metrics

67
Citations
Jump to Citations
2
Data citations
Jump to Data

Citations of article over time

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1084/jem.143.5.1131

Supporting
Mentioning
Contrasting
1
27
0

Article citations

Go to all (67) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.