Interleukin (Il)-4 Is a Major Regulatory Cytokine Governing Bioactive IL-12 Production by Mouse and Human Dendritic Cells

doi:10.1084/jem.192.6.823

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J Exp Med. 2000 Sep 18; 192(6): 823–834.

doi: 10.1084/jem.192.6.823

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Table 2

IL-12 p70 and p40 Production by Isolated Human Thymic DCs in the Presence or Absence of IL-4

Experiment
1	p70 (pg/ml)	260	542
	p40 (pg/ml)	12,992	5,440
2	p70 (pg/ml)	6	76
	p40 (pg/ml)	906	391
	IL-4	−	+

Human thymic DCs (9 × 10⁴ DCs per 200 μl in experiment 1, or 5 × 10⁴ DCs per 200 μl in experiment 2) were incubated with CD40L in the presence of GM-CSF and IFN-γ with or without IL-4 for 3 d (experiment 1) or 2 d (experiment 2). Supernatants were assayed for IL-12 p70 and p40 by ELISA.