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Bioconjugate Chemistry
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Table 1

Reagents Used as Immunogens and as Antigens and Competitors in ELISAs
Immunogens
 polymer component
protein componentend groupPEG M.W.# of PEGs
human interferon-αmethoxy20 kDa1
20 kDa2
hydroxy20 kDa1
20 kDa2
porcine uricasemethoxy10 kDa2.3a
hydroxy10 kDa2.3a
human serum albuminmethoxy10 kDa17
t-butoxy10 kDa17
Antigens
 polymer component
protein componentend groupPEG M.W.# of PEGs
porcine superoxide dismutase (SOD)methoxy10 kDa2.5b
hydroxy10 kDa2.5b
t-butoxy10 kDa2.5b
human interferon-α(No PEG)  
Competitors
free polymersmPEG10 kDa
20 kDa
PEG Diol10 kDa
20 kDa
 polymer component
PEG-albumin conjugatesend groupPEG M.W.# of PEGs
 methoxy10 kDa17–19
hydroxy10 kDa17–22
aNumber of PEGs per uricase subunit.
bNumber of PEGs per SOD subunit.