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Alternative use of chromosome fragmentation sites in the ciliated protozoan Oxytricha nova.

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  • 1. Department of Biochemistry, University of Connecticut Health Center, Farmington 06032.
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    • Klobutcher LA 1
    (1 author)

ORCIDs linked to this article

Nucleic Acids Research, 01 Jan 1988, 16(1):251-264
https://doi.org/10.1093/nar/16.1.251 PMID: 2829118 PMCID: PMC334624

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Abstract 

During its life cycle, the hypotrichous ciliated protozoan Oxytricha nova transforms a copy of its micronucleus, which contains chromosome-sized DNA, into a macronucleus containing linear, gene-sized DNA molecules. A region of the micronuclear genome has been defined that gives rise to two distinct macronuclear DNA molecules during development. Through analysis of recombinant macronuclear and micronuclear clones, the generation of the two macronuclear DNA molecules was shown to be the result of alternative use of chromosome fragmentation sites. In addition, evidence was obtained that adjacent micronuclear precursors of macronuclear DNA molecules can overlap by a few base pairs. The significance of these findings in relation to developmental chromosome fragmentation is discussed.

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Nucleic Acids Res. 1988 Jan 11; 16(1): 251–264.
PMCID: PMC334624
PMID: 2829118

Alternative use of chromosome fragmentation sites in the ciliated protozoan Oxytricha nova.

L A Klobutcher, M E Huff, and G E Gonye
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Abstract

During its life cycle, the hypotrichous ciliated protozoan Oxytricha nova transforms a copy of its micronucleus, which contains chromosome-sized DNA, into a macronucleus containing linear, gene-sized DNA molecules. A region of the micronuclear genome has been defined that gives rise to two distinct macronuclear DNA molecules during development. Through analysis of recombinant macronuclear and micronuclear clones, the generation of the two macronuclear DNA molecules was shown to be the result of alternative use of chromosome fragmentation sites. In addition, evidence was obtained that adjacent micronuclear precursors of macronuclear DNA molecules can overlap by a few base pairs. The significance of these findings in relation to developmental chromosome fragmentation is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Swanton MT, Heumann JM, Prescott DM. Gene-sized DNA molecules of the macronuclei in three species of hypotrichs: size distributions and absence of nicks. DNA of ciliated protozoa. VIII. Chromosoma. 1980;77(2):217–227. [Abstract] [Google Scholar]
  • Kraut H, Lipps HJ, Prescott DM. The genome of hypotrichous ciliates. Int Rev Cytol. 1986;99:1–28. [Abstract] [Google Scholar]
  • Klobutcher LA, Jahn CL, Prescott DM. Internal sequences are eliminated from genes during macronuclear development in the ciliated protozoan Oxytricha nova. Cell. 1984 Apr;36(4):1045–1055. [Abstract] [Google Scholar]
  • Pluta AF, Dani GM, Spear BB, Zakian VA. Elaboration of telomeres in yeast: recognition and modification of termini from Oxytricha macronuclear DNA. Proc Natl Acad Sci U S A. 1984 Mar;81(5):1475–1479. [Europe PMC free article] [Abstract] [Google Scholar]
  • Ribas-Aparicio RM, Sparkowski JJ, Proulx AE, Mitchell JD, Klobutcher LA. Nucleic acid splicing events occur frequently during macronuclear development in the protozoan Oxytricha nova and involve the elimination of unique DNA. Genes Dev. 1987 Jun;1(4):323–336. [Abstract] [Google Scholar]
  • Klobutcher LA, Vailonis-Walsh AM, Cahill K, Ribas-Aparicio RM. Gene-sized macronuclear DNA molecules are clustered in micronuclear chromosomes of the ciliate Oxytricha nova. Mol Cell Biol. 1986 Nov;6(11):3606–3613. [Europe PMC free article] [Abstract] [Google Scholar]
  • Swanton MT, Greslin AF, Prescott DM. Arrangement of coding and non-coding sequences in the DNA molecules coding for rRNAs in Oxytricha sp. DNA of ciliated protozoa. VII. Chromosoma. 1980;77(2):203–215. [Abstract] [Google Scholar]
  • Lauth MR, Spear BB, Heumann J, Prescott DM. DNA of ciliated protozoa: DNA sequence diminution during macronuclear development of Oxytricha. Cell. 1976 Jan;7(1):67–74. [Abstract] [Google Scholar]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [Abstract] [Google Scholar]
  • Boswell RE, Klobutcher LA, Prescott DM. Inverted terminal repeats are added to genes during macronuclear development in Oxytricha nova. Proc Natl Acad Sci U S A. 1982 May;79(10):3255–3259. [Europe PMC free article] [Abstract] [Google Scholar]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [Europe PMC free article] [Abstract] [Google Scholar]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [Abstract] [Google Scholar]
  • Herrick G, Cartinhour SW, Williams KR, Kotter KP. Multiple sequence versions of the Oxytricha fallax 81-MAC alternate processing family. J Protozool. 1987 Nov;34(4):429–434. [Abstract] [Google Scholar]
  • Austerberry CF, Allis CD, Yao MC. Specific DNA rearrangements in synchronously developing nuclei of Tetrahymena. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7383–7387. [Europe PMC free article] [Abstract] [Google Scholar]
  • Howard EA, Blackburn EH. Reproducible and variable genomic rearrangements occur in the developing somatic nucleus of the ciliate Tetrahymena thermophila. Mol Cell Biol. 1985 Aug;5(8):2039–2050. [Europe PMC free article] [Abstract] [Google Scholar]
  • Baroin A, Prat A, Caron F. Telomeric site position heterogeneity in macronuclear DNA of Paramecium primaurelia. Nucleic Acids Res. 1987 Feb 25;15(4):1717–1728. [Europe PMC free article] [Abstract] [Google Scholar]
  • Cartinhour SW, Herrick GA. Three different macronuclear DNAs in Oxytricha fallax share a common sequence block. Mol Cell Biol. 1984 May;4(5):931–938. [Europe PMC free article] [Abstract] [Google Scholar]
  • Green MR. Pre-mRNA splicing. Annu Rev Genet. 1986;20:671–708. [Abstract] [Google Scholar]
  • Hood L, Kronenberg M, Hunkapiller T. T cell antigen receptors and the immunoglobulin supergene family. Cell. 1985 Feb;40(2):225–229. [Abstract] [Google Scholar]
  • Yao MC, Zheng K, Yao CH. A conserved nucleotide sequence at the sites of developmentally regulated chromosomal breakage in Tetrahymena. Cell. 1987 Mar 13;48(5):779–788. [Abstract] [Google Scholar]
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NIGMS NIH HHS (1)