Europe PMC

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Interaction of the operator of the tryptophan operon with repressor.

Proceedings of the National Academy of Sciences of the United States of America, 01 Aug 1974, 71(8):3134-3138
https://doi.org/10.1073/pnas.71.8.3134 PMID: 4528605 PMCID: PMC388636

Free full text in Europe PMC

Abstract 

Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo. The half-life of the trp repressor-trp operator complex is less than 2 min in vitro in 0.12 M salt.

Free full text 

Logo of pnasLink to Publisher's site
Proc Natl Acad Sci U S A. 1974 Aug; 71(8): 3134–3138.
PMCID: PMC388636
PMID: 4528605

Interaction of the Operator of the Tryptophan Operon with Repressor

John K. Rose and Charles Yanofsky
Author information Copyright and License information Disclaimer

Abstract

Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37°, a value consistent with the extent of trp operon regulation in vivo. The half-life of the trp repressor-trp operator complex is less than 2 min in vitro in 0.12 M salt.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.0M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.
 
icon of scanned page 3134
3134
icon of scanned page 3135
3135
icon of scanned page 3136
3136
icon of scanned page 3137
3137
icon of scanned page 3138
3138
 

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Zubay G, Morse DE, Schrenk WJ, Miller JH. Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc Natl Acad Sci U S A. 1972 May;69(5):1100–1103. [Europe PMC free article] [Abstract] [Google Scholar]
  • Rose JK, Squires CL, Yanofsky C, Yang HL, Zubay G. Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan. Nat New Biol. 1973 Oct 3;245(144):133–137. [Abstract] [Google Scholar]
  • McGeoch D, McGeoch J, Morse D. Synthesis of tryptophan operon RNA in a cell-free system. Nat New Biol. 1973 Oct 3;245(144):137–140. [Abstract] [Google Scholar]
  • Shimizu Y, Shimizu N, Hayashi M. In vitro repression of transcription of the tryptophan operon by trp repressor. Proc Natl Acad Sci U S A. 1973 Jul;70(7):1990–1994. [Europe PMC free article] [Abstract] [Google Scholar]
  • Squires CL, Rose JK, Yanofsky C, Yang HL, Zubay G. Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon. Nat New Biol. 1973 Oct 3;245(144):131–133. [Abstract] [Google Scholar]
  • Doolittle WF, Yanofsky C. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase. J Bacteriol. 1968 Apr;95(4):1283–1294. [Europe PMC free article] [Abstract] [Google Scholar]
  • Mosteller RD, Yanofsky C. Evidence that tryptophanyl transfer ribonucleic acid is not the corepressor of the tryptophan operon of Escherichia coli. J Bacteriol. 1971 Jan;105(1):268–275. [Europe PMC free article] [Abstract] [Google Scholar]
  • Riggs AD, Suzuki H, Bourgeois S. Lac repressor-operator interaction. I. Equilibrium studies. J Mol Biol. 1970 Feb 28;48(1):67–83. [Abstract] [Google Scholar]
  • Jackson EN, Yanofsky C. Internal promoter of the tryptophan operon of Escherichia coli is located in a structural gene. J Mol Biol. 1972 Aug 21;69(2):307–313. [Abstract] [Google Scholar]
  • Riggs AD, Bourgeois S, Cohn M. The lac repressor-operator interaction. 3. Kinetic studies. J Mol Biol. 1970 Nov 14;53(3):401–417. [Abstract] [Google Scholar]
  • Jobe A, Riggs AD, Bourgeois S. Lac repressor-operator interaction. V. Characterization of super- and pseudo-wild-type repressors. J Mol Biol. 1972 Feb 28;64(1):181–199. [Abstract] [Google Scholar]
  • Riggs AD, Newby RF, Bourgeois S. lac repressor--operator interaction. II. Effect of galactosides and other ligands. J Mol Biol. 1970 Jul 28;51(2):303–314. [Abstract] [Google Scholar]
  • Gilbert W, Müller-Hill B. The lac operator is DNA. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415–2421. [Europe PMC free article] [Abstract] [Google Scholar]
  • LUBIN M, ENNIS HL. ON THE ROLE OF INTRACELLULAR POTASSIUM IN PROTEIN SYNTHESIS. Biochim Biophys Acta. 1964 Apr 27;80:614–631. [Abstract] [Google Scholar]
  • Parks JS, Gottesman M, Shimada K, Weisberg RA, Perlman RL, Pastan I. Isolation of the gal repressor. Proc Natl Acad Sci U S A. 1971 Aug;68(8):1891–1895. [Europe PMC free article] [Abstract] [Google Scholar]
Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

Full text links 

Read article at publisher's site: https://doi.org/10.1073/pnas.71.8.3134

Read article for free, from open access legal sources, via Unpaywall: https://europepmc.org/articles/pmc388636?pdf=renderUnpaywall

Citations & impact 

Impact metrics

36
Citations
Jump to Citations
1
Data citation
Jump to Data

Citations of article over time

Article citations

Go to all (36) article citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.