Monocyte recruitment during infection and inflammation.

Shi C; Pamer EG

doi:10.1038/nri3070

Monocyte recruitment during infection and inflammation.

Shi C ¹,

Pamer EG

Affiliations

1. Infectious Diseases Service, Department of Medicine, Immunology Program, Sloan-Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.
Authors
Shi C¹
(1 author)

Nature reviews. Immunology, 10 Oct 2011, 11(11):762-774
https://doi.org/10.1038/nri3070 PMID: 21984070 PMCID: PMC3947780

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Abstract

Monocytes originate from progenitors in the bone marrow and traffic via the bloodstream to peripheral tissues. During both homeostasis and inflammation, circulating monocytes leave the bloodstream and migrate into tissues where, following conditioning by local growth factors, pro-inflammatory cytokines and microbial products, they differentiate into macrophage or dendritic cell populations. Recruitment of monocytes is essential for effective control and clearance of viral, bacterial, fungal and protozoal infections, but recruited monocytes also contribute to the pathogenesis of inflammatory and degenerative diseases. The mechanisms that control monocyte trafficking under homeostatic, infectious and inflammatory conditions are being unravelled and are the focus of this Review.

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Nat Rev Immunol. Author manuscript; available in PMC 2014 Mar 9.

Published in final edited form as:

Nat Rev Immunol. 2011 Oct 10; 11(11): 762–774.

Published online 2011 Oct 10. https://doi.org/10.1038/nri3070

PMCID: PMC3947780

NIHMSID: NIHMS556423

PMID: 21984070

Monocyte recruitment during infection and inflammation

Chao Shi and Eric G. Pamer

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Abstract

Monocytes are a subset of circulating white blood cells that can further differentiate into a range of tissue macrophages and dendritic cells (DCs)¹. Bloodstream monocytes are derived from precursors in the bone marrow² and are subdivided into subsets that differ in size, trafficking and innate immune receptor expression and in their ability to differentiate following stimulation with cytokines and/or microbial molecules¹. Monocytes mediate host antimicrobial defence³ and are also implicated in many inflammatory diseases, including atherosclerosis⁴. In addition, monocytes can be recruited to tumour sites and can inhibit tumour-specific immune defence mechanisms⁵.

Studies of these different diseases have revealed the remarkable multipotency of monocytes in different inflammatory environments. The ability of monocytes to mobilize and traffic to where they are needed is central for their functions in promoting immune defence during infection and in driving inflammatory diseases. This Review focuses on the mechanisms that allow monocytes to traffic from their site of origin — the bone marrow — to distinct tissue sites. We discuss the mechanisms involved in homeostatic trafficking of monocytes and in their ‘patrolling’ functions, and compare these with the mechanisms that are used to recruit monocytes to inflamed tissues.

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Monocyte subsets and chemokine receptors

Monocytes are divided into subsets on the basis of chemokine receptor expression and the presence of specific surface molecules^6,7. In mice, expression of LY6C and CD11b identifies a monocyte subset that expresses high levels of CC-chemokine receptor 2 (CCR2) but low levels of CX₃C-chemokine receptor 1 (CX₃CR1). These monocytes — which are often referred to as inflammatory or LY6C^hi monocytes — represent approximately 2–5% of circulating white blood cells in an uninfected mouse and are rapidly recruited to sites of infection and inflammation³. CCR2 deficiency markedly reduces LY6C^hi monocyte trafficking to sites of inflammation, indicating a crucial role for this chemokine receptor in the trafficking of these monocytes^8,9. CCR2 is also expressed by haematopoietic stem cells (HSCs) and a subset of natural killer (NK) cells, and CCR2-mediated recruitment of HSCs contributes to the generation of inflammatory monocyte populations during peritonitis and acetaminophen-induced hepatitis¹⁰.

A second major subset of circulating monocytes in mice expresses high levels of CX₃CR1 and low levels of CCR2 and LY6C^6,7. In the bloodstream, these LY6C^low monocytes (also referred to as CX₃CR1^hi monocytes) are less prevalent than LY6C^hi monocytes, and intravital microscopy studies demonstrate that they adhere to and migrate along the luminal surface of endothelial cells that line small blood vessels in a process referred to as patrolling¹¹ (FIG. 1).

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Figure 1

Trafficking of circulating monocyte subsets

Macrophage and dendritic cell precursors (MDPs) in the bone marrow give rise to LY6C^hi monocytes, which can serve as an intermediate for LY6C^low monocyte generation. LY6C^hi monocytes exit the bone marrow in a CC-chemokine receptor 2 (CCR2)-dependent manner and are recruited to inflamed tissues. Here, they can differentiate into TNF- and iNOS-producing dendritic cells (TIP DCs), inflammatory macrophages or inflammatory DCs, some of which can subsequently migrate to draining lymph nodes. LY6C^low monocytes patrol the blood vessel lumen by associating with the vascular endothelium. LY6C^low monocytes are also recruited to sites of inflammation and possibly contribute to wound healing by differentiating into alternatively activated macrophages. Under certain experimental conditions, LY6C^hi monocytes can give rise to Langerhans cells in the skin, microglia in the central nervous system, and CX₃C-chemokine receptor 1 (CX₃CR1)⁺ mononuclear phagocytes in the intestinal lamina propria, where they can sample the gut lumen by extending dendrites between intestinal epithelial cells. In the absence of inflammation, some circulating LY6C^hi monocytes return to the bone marrow. The spleen functions as a reservoir for monocytes outside the bone marrow. Following myocardial infarction, monocytes are released from splenic reservoirs, and LY6C^hi monocytes are selectively recruited to the injured heart.

Human monocytes are divided into subsets on the basis of surface CD14 and CD16 expression¹². CD14⁺⁺CD16⁻ monocytes, which are also referred to as classical monocytes¹³, are the most prevalent monocyte subset in human blood and, similarly to mouse LY6C^hi monocytes, these cells express CCR2 (REF. 7). The CD16⁺ monocyte population comprises two subsets, CD14⁺CD16⁺⁺ and CD14⁺⁺CD16⁺ monocytes; these are also referred to as non-classical and intermediate monocytes, respectively¹³. The CD14⁺CD16⁺⁺ subset, in terms of in vivo patrolling, is similar to the mouse LY6C^low monocyte population¹⁴ (TABLE 1). Although the monocyte subsets identified in humans and mice are not precisely overlapping, their differentiation and contribution to immune defence appear to be similar^15–17.

Table 1

Mouse and human monocyte subsets

Subset	Markers	Chemokine receptors	Functions
*Mouse*
LY6C^hi	CD11b⁺CD115⁺LY6C^hi	CCR2^hiCX₃CR1^low	Pro-inflammatory⁷ and antimicrobial³ roles
LY6C^low	CD11b⁺CD115⁺LY6C^low	CX₃CR1^hiCCR2^low	Patrolling¹¹; early responses¹¹; tissue repair¹²⁸
*Human*
Classical	CD14⁺⁺CD16⁻	CCR2^hiCX₃CR1^low	Resemble LY6C^hi monocytes based on gene-expression arrays^7,17,140
Intermediate	CD14⁺⁺CD16⁺	CX₃CR1^hiCCR2^low	Pro-inflammatory roles^12,15
Non-classical	CD14⁺CD16⁺⁺	CX₃CR1^hiCCR2^low	Patrolling¹⁴; antiviral roles¹⁴

CCR2, CC-chemokine receptor 2; CX₃CR1, CX₃C-chemokine receptor 1.

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Chemokines and monocyte trafficking

CCR2 and its ligands

CC–chemokine ligand 2 (CCL2; also known as MCP1) and CCL7 (also known as MCP3) are CC-chemokines that bind to CCR2 and mediate LY6C^hi monocyte recruitment¹⁸. Although expression of CCR2 is restricted to only a few cell types, most (if not all) nucleated cells express CCL2 in response to activation by pro-inflammatory cytokines or stimulation of innate immune receptors by a range of microbial molecules^18–22. Many infections induce the expression of CCL2 and result in high circulating levels of CCL2 in serum and within inflamed tissues.

Although CCL2 is important for monocyte recruitment in vivo, its mechanism of action remains unclear. One idea, which is based on the finding that CCL2 dimerizes and associates with tissue glycosaminoglycans, is that circulating CCL2 associates with glycosaminoglycans in specific tissues and establishes gradients that guide monocytes towards sites of infection or inflammation. In support of this model, monocyte recruitment is impaired by amino acid substitutions in CCL2 that prevent dimerization or association with glycosaminoglycans^23,24. CCL2 has been suggested to circulate in the lymph and bind to high endothelial venules in lymph nodes, thereby guiding monocytes into the lymph nodes⁶. Other studies have demonstrated that the Duffy antigen receptor for chemokines (DARC) — a non-signalling chemokine receptor that binds CCL2 and transports it across endothelial cells to the luminal side — is important for the recruitment of monocytes from the bloodstream into inflamed tissues²⁵.

CCL7 is also induced by bacterial infection and contributes to LY6C^hi monocyte recruitment^18,26. Deletion of either Ccl2 or Ccl7 diminishes the recruitment of monocytes during infection by approximately 40–50%²⁶, although the specific contributions of CCL2 and CCL7 to the process of recruitment remain unclear. One possibility is that CCL2 and CCL7 act in series within tissues, guiding monocytes to sequential sites in the bone marrow before they enter the bloodstream. Alternatively, CCL2 and CCL7 may act in parallel, driving monocyte recruitment from different regions within the bone marrow or other tissues. CCL8 and CCL12 also bind to CCR2, but deletion of the genes encoding these chemokines does not have a detectable impact on monocyte trafficking under homeostatic conditions¹⁸.

The CX₃CL1–CX₃CR1 axis

LY6C^low monocytes respond to CX₃C-chemokine ligand 1 (CX₃CL1; also known as fractalkine), a membrane-bound chemokine that is expressed in tissues and the marginal zone of the spleen. Deletion of CX3CR1 results in diminished patrolling by LY6C^low monocytes¹¹ and also decreases the recruitment of LY6C^hi monocytes to splenic sites of bacterial infection²⁷. CX₃CL1 expression was detected in the marginal zone of the spleen during bacterial infection and, as had been previously proposed, CX₃CL1 mediated very early recruitment of LY6C^low monocytes to the spleen²⁷. In addition to their role in monocyte trafficking, CX₃CL1 and CX₃CR1 provide a survival signal to LY6C^low monocytes under both steady-state and inflammatory conditions²⁸.

CCR1 and CCR5

Monocytes also express CCR1 and CCR5 (REFS ²⁹^,³⁰), and these receptors bind to a variety of chemokines, including the shared ligands CCL3 (also known as MIP1α) and CCL5 (REF. 31). In vitro transmigration assays have shown that CCR1 predominantly mediates the arrest of monocytes in the presence of shear flow, CCR5 contributes to monocyte ‘spreading’, and both CCR1 and CCR5 support transendothelial chemotaxis towards CCL5; this suggests that the two receptors have specialized roles in monocyte recruitment³². In vivo studies using mice deficient in CCR1 or CCR5 have demonstrated non-redundant roles for these chemokine receptors in inflammatory cell recruitment^33–38 with implications for the development and/or progression of atherosclerosis, multiple sclerosis and rheumatoid arthritis³¹. CCR5-mediated signals, for example, contribute to the pathogenesis of atherosclerosis^39,40. The recruitment of LY6C^hi monocytes to atherosclerotic plaques in apolipoprotein E-deficient (Apoe^−/−) mice is mediated in part by CCR2, CCR5 and CX₃CR1. Recruitment of LY6C^low monocytes is partially dependent on CCR5, which is upregulated in the LY6C^low monocyte population in Apoe^−/− mice⁴¹. In multiple sclerosis, infiltrating monocytes express both CCR1 and CCR5 (REFS ⁴²^,⁴³), and the ligands for these receptors are present in the affected tissues^44,45. Mice deficient in CCR1 are more resistant to the development of experimental autoimmune encephalomyelitis (EAE), a mouse model of multiple sclerosis⁴⁶. These findings suggest that CCR1 and CCR5 may contribute to the recruitment of monocytes into or within inflamed tissues. Determining the role of CCR1 and CCR5 in monocyte recruitment is complicated by the fact that these chemokine receptors, unlike CCR2, are expressed by a wide range of cell types^47–50. Thus, alterations in monocyte recruitment in CCR1− or CCR5-deficient mice may be secondary to defective recruitment of other cell populations.

CCR6, CCR7, CCR8 and CXCR2

Though less studied, CCR6, CCR7, CCR8 and CXC-chemokine receptor 2 (CXCR2) have been suggested to be involved in monocyte recruitment. Circulating monocytes do not express significant levels of CCR6 (REF. 7) and do not respond to CCL20 (the ligand for CCR6) in vitro⁵¹. Therefore, it is unlikely that CCR6 mediates extravasation of circulating monocytes into tissues. However, transfer of CCR6⁺ (but not CCR6⁻) LY6C^hi monocytes restored the functional defects in Ccr6^−/− mice in a model of epithelial immunization⁵² and in a model of intradermal infection with Salmonella enterica⁵³, suggesting a role for CCR6 in the migration or function of monocytes in inflamed tissues. Blood GR1^mid monocytes express Ccr7 and Ccr8 mRNAs and give rise to DCs that subsequently use CCR7 and CCR8 to migrate from the skin to the lymph nodes⁵⁴. CXCR2 mediates monocyte arrest that is induced by macrophage migration inhibitory factor (MIF)⁵⁵.

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Adhesion molecules in monocyte trafficking

Monocyte recruitment is thought to follow the general paradigm of leukocyte adhesion and trafficking, which involves rolling, adhesion and transmigration. The migration of leukocytes, including monocytes, depends on integrins and other adhesion molecules⁵⁶. LY6C^hi monocytes in mice express L-selectin (also known as CD62L), P-selectin glycoprotein ligand 1 (PSGL1), lymphocyte function-associated antigen 1 (LFA1; also known as αLβ2 integrin), macrophage receptor 1 (MAC1; also known as integrin αMβ2), platelet endothelial cell adhesion molecule (PECAM1) and very late antigen 4 (VLA4; also known as integrin α4β1), which all contribute to leukocyte adhesion and migration.

Monocyte recruitment during thioglycollate-induced peritonitis is impaired in mice deficient in L-selectin⁵⁷. Using antibody-mediated blockade of adhesion molecules, it was shown that, in mice infected with Leishmania major, monocyte migration through inflamed dermal venules depends on two sets of interactions: PSGL1 expressed on monocytes interacts with P-selectin and E-selectin expressed on the venule endothelium; and monocyte-expressed L-selectin binds to endothelial cell-expressed peripheral node addressin (PNAd; a mixture of glycoprotein ligands for L-selectin)⁵⁸. Monocyte migration through lymph node high endothelial venules also relies on L-selectin–PNAd interactions⁵⁸. In the setting of atherosclerosis, P-selectin and E-selectin mediate the rolling of monocytes on inflamed aortic endothelium, whereas vascular cell adhesion molecule 1 (VCAM1) contributes to slow rolling and tight adhesion⁵⁹. The involvement of intercellular adhesion molecule 1 (ICAM1), PECAM1, endothelial junctional proteins and other vascular adhesion molecules in the recruitment of monocytes to atherosclerotic aortas has been well covered in previous reviews^60,61. Administration of antibodies that block MAC1 (REF. 62) or ICAM1 (REF. 63) also inhibits inflammatory monocyte recruitment during acute inflammation.

Patrolling by LY6C^low monocytes along endothelial cells in resting dermal blood vessels was shown to be mediated by the integrin LFA1 (REF. 11). By contrast, the early recruitment of LY6C^hi monocytes is not affected by LFA1 deficiency⁶⁴. These results suggest that the adhesion mechanisms used by distinct monocyte subsets may differ, depending on the type of tissue and the inflammatory state. A summary of molecules involved in monocyte recruitment, which is not meant to be exhaustive, is shown in TABLE 2.

Table 2

Molecules involved in monocyte trafficking

Monocyte-expressed molecules	Binding partners	Functions in monocyte recruitment
*Chemokines and their receptors*
CCR2	CCL2, CCL7 and CCL12	Emigration of LY6C^hi monocytes from the bone marrow^18,26
CX₃CR1	CX₃CL1	Patrolling; recruitment to splenic sites of bacterial infection²⁷
CCR1 and CCR5	Various, including the shared ligands CCL3 and CCL5	Recruitment into or within inflamed tissues^41–43,125
CCR6	CCL20	Possible role in the migration or function of LY6C^hi monocytes in inflamed tissues^52,53
CCR7 and CCR8	CCL19 and CCL1, respectively	Migration of monocyte-derived DCs from the skin to lymph nodes⁵⁴
CXCR2	MIF	Arrest in atherosclerotic arteries of mice⁵⁵
*Adhesion molecules*
L-selectin	Glycoproteins, including CD34, GLYCAM1 and MADCAM1	Tethering and rolling^56,57; recruitment during thioglycollate-induced peritonitis⁵⁷; migration to lymph nodes⁵⁸
PSGL1	P-selectin and E-selectin	Migration through inflamed dermal venules⁵⁸; tethering and rolling on atherosclerotic endothelium⁶¹
LFA1	ICAM1	Patrolling during the steady state; not involved in the early recruitment of inflammatory monocytes¹¹
MAC1	ICAM1	Adhesion during acute inflammation^62,63
VLA4	VCAM1	Adhesion to inflamed endothelium^60,61
PECAM1	Endothelial PECAM1	Transendothelial migration^141,142

CCL, CC-chemokine ligand; CCR, CC-chemokine receptor; CX₃CL1, CX₃C-chemokine ligand 1; CX₃CR1, CX₃C-chemokine receptor 1; CXCR2, CXC-chemokine receptor 2; DC, dendritic cell; GLYCAM1, glycosylation-dependent cell adhesion molecule 1; ICAM1, intercellular adhesion molecule 1; LFA1, lymphocyte function-associated antigen 1; MAC1, macrophage receptor 1; MADCAM1, mucosal addressin cell adhesion molecule 1; MIF, macrophage migration inhibitory factor; PECAM1, platelet endothelial cell adhesion molecule; PSGL1, P-selectin glycoprotein ligand 1; VCAM1, vascular cell adhesion molecule 1; VLA4, very late antigen 4.

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Constitutive trafficking of monocytes

Adoptively transferred CX₃CR1⁺ (LY6C^low) blood monocytes patrol blood vessels and enter non-inflamed tissues, whereas LY6C^hi monocytes selectively traffic to sites of inflammation⁷. In the absence of inflammation, LY6C^hi monocytes return to the bone marrow, where they are thought to differentiate into LY6C^low monocytes^65,66 (FIG. 1). Under normal circumstances, peripheral tissues are replete with the progeny of circulating monocytes, and it is difficult, on an experimental level, to detect replenishment of tissue-resident macrophages and DCs by circulating monocytes. Depletion of resident cells by a variety of experimental approaches has been used to demonstrate that, under certain conditions, circulating monocytes can reconstitute Langerhans cell populations in the skin⁶⁷, microglia in the brain^68,69 and CX₃CR1⁺ mononuclear phagocytes in the intestinal lamina propria^70,71. It is unclear whether the loss of these cells in tissues enhances monocyte recruitment (for example, by inducing the expression of chemokines or the upregulation of adhesion molecules) or simply opens up a niche that monocytes occupy. It is important to note that monocyte-mediated replenishment of tissue-resident cell populations following their experimental depletion does not necessarily reflect the natural processes that occur during the development of different tissues or under homeostatic conditions. For example, in the steady state, Langerhans cells are maintained by local self-renewal rather than through replenishment by blood-borne cells⁷². Also, during embryonic development, microglia develop from primitive myeloid progenitors that are distinct from inflammatory monocytes⁷³. Nevertheless, the ability of monocytes to reconstitute distinct tissue macrophage and DC populations suggests that this may be a physiologically important and relevant role for monocytes following tissue trauma or infection.

Macrophage and DC progenitors (MDPs) give rise to monocyte progenitors and also to common DC progenitors (CDPs)⁷⁴. Monocyte progenitors express CD115 (also known as the M-CSF receptor) and develop in response to macrophage colony-stimulating factor (M-CSF), whereas CDP development is driven by FMS-related tyrosine kinase 3 ligand (FLT3L). LY6C^hi monocytes traffic to the intestinal lamina propria by mechanisms that remain unclear and differentiate into CD11b⁺CD11c⁺ DCs that also express CX₃CR1 (which have been referred to as CX₃CR1⁺ mononuclear phagocytes)⁷⁵, whereas CDPs give rise to intestinal CD103⁺ DCs^70,71. Following the depletion of CD11c^hi DCs in mice, LY6C^hi monocytes are recruited to the lamina propria and undergo extensive proliferation to ‘fill the space’⁷⁰. The resulting CX₃CR1⁺ mononuclear phagocytes extend dendrites between intestinal epithelial cells, a process that depends on CX₃CR1, and enhance the uptake of bacteria into underlying tissues⁷⁶. CX₃CR1⁺ mononuclear phagocytes, however, do not traffic to draining lymph nodes and thus are not thought to directly mediate T cell priming. Instead, CD103⁺ DCs, which express high levels of CCR7, carry antigen into draining mesenteric lymph nodes, where they prime T cells^71,77.

Careful kinetic studies of adoptively transferred monocytes have demonstrated that, in the lung, LY6C^hi monocytes give rise to CD103⁺ DCs, whereas LY6C^low monocytes give rise to CD11b⁺ DCs⁷⁸. However, it has also been suggested that CD103⁺ DCs in the lung can be derived from CDPs⁷⁹. LY6C^low monocytes also give rise to alveolar macrophages after first differentiating into parenchymal macrophages in the lung^80,81.

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Monocyte recruitment to sites of infection

In addition to their role in replenishing tissue macrophages and DCs in the steady state, monocytes are recruited to infected tissues and mediate direct antimicrobial activity at these sites³. During infection, monocytes and/or monocyte-derived cells can also enter draining lymph nodes and promote adaptive immune responses (BOX 1). Studies with infectious disease models in mice are providing insights into the mechanisms that mobilize and target monocytes to sites of microbial infection. LY6C^low monocytes can be recruited to sites of infection even earlier than LY6C^hi monocytes, and can participate in the initial inflammatory response by releasing tumour necrosis factor (TNF) and chemokines¹¹. However, in most cases of infection, the ensuing recruitment of LY6C^hi monocytes is more prominent and robust. Because few studies using infection models have included a comparative analysis on the recruitment of different monocyte subsets, the relative contributions of LY6C^hi versus LY6C^low monocytes in these settings are not adequately defined. In the following sections, we review some of the major infectious disease models in which a role for CCR2-mediated monocyte recruitment has been identified (FIG. 2).

Box 1

Monocyte recruitment to lymph nodes during infection

Although lymphoid tissues can be important sites of infection, most microorganisms invade non-lymphoid tissues and, in the process, cause tissue damage and induce inflammatory responses. Although some evidence supports the direct recruitment of monocytes to lymph nodes via high endothelial venules^6,120, the predominant route by which inflammatory monocytes contribute to immune defence is by direct recruitment to the infected tissue and subsequent entry into lymph nodes via afferent lymphatic channels^100,138. Pro-inflammatory signals (including pro-inflammatory cytokines and microbial molecules) from the site of infection induce monocyte emigration from the bone marrow, and circulating LY6C^hi monocytes are targeted to inflammatory sites in large part by the upregulation of adhesion molecules on local endothelial cells. Monocytes have a direct antimicrobial role at sites of infection and can also, particularly during pulmonary or dermal infections, carry microbial antigens to local lymph nodes, where they can either transfer antigens to classical dendritic cells (DCs)¹¹¹ or differentiate into monocyte-derived DCs. Recent studies have shown that monocyte-derived DCs can be as effective as classical DCs in priming CD4⁺ and CD8⁺ T cells¹³⁹, suggesting that monocytes may contribute to pathogen-specific T cell responses at multiple stages of the immune response.

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Figure 2

Monocyte recruitment is crucial for defence against a broad range of pathogenic microorganisms

Infections with diverse pathogens, including bacteria, parasites, fungi and viruses, induce the recruitment of LY6C^hi monocytes to sites of infection. For most pathogens (including those listed in the blue boxes), CC-chemokine receptor 2 (CCR2)-mediated monocyte recruitment enhances resistance to infection. However, during some infections (such as Trypanosoma brucei or influenza virus infection; listed in the red boxes), CCR2-mediated recruitment of monocytes is deleterious and enhances pathology.

Bacterial infections

Listeria monocytogenes is a facultative intracellular Gram-positive bacterium that infects mammals and, particularly in rabbits, induces monocytosis⁸². Inflammatory cell recruitment to the liver and spleen — two major sites of infection — is essential for early control of L. monocytogenes, and antibody-mediated blockade of CD11b reduces bacterial clearance and increases mortality⁸³. CCR2 deficiency worsens infection with L. monocytogenes and reduces the recruitment of LY6C^hi monocytes to infected tissues^8,84. TNF and inducible nitric oxide synthase (iNOS) both contribute to innate defence against L. monocytogenes and are produced by TNF- and iNOS-producing (TIP) DCs, a cell population that is derived from LY6C^hi monocytes that are recruited to the foci of infection⁸⁴. LY6C^hi monocytes also give rise to iNOS-producing cells following infection with Brucella melitensis⁸⁵, Mycobacterium tuberculosis and various protozoa (see below). Infection with L. monocytogenes induces CCL2 and CCL7 expression, and these chemokines, at later stages of infection, can be detected in the serum, spleen, liver and lungs²⁶. Monocyte recruitment following L. monocytogenes infection is reduced in mice lacking CCL2 or CCL7. Mice deficient in CCL2, CCL7 or CCR2 retain LY6C^hi monocytes in the bone marrow, indicating that CCR2-mediated signals are principally required for LY6C^hi monocyte recruitment from the bone marrow into the bloodstream⁸⁶. Adoptive transfer of CCR2-deficient monocytes into L. monocytogenes-infected mice demonstrated that the trafficking of these monocytes to sites of infection in the spleen is normal⁸⁶. Trafficking of LY6C^hi monocytes to foci of L. monocytogenes infection in the liver is also CCR2 independent and predominantly mediated by CD11b, ICAM1 and CD44 (REF. 63).

Monocyte emigration from the bone marrow during the first 72 hours of L. monocytogenes infection can occur in the absence of myeloid differentiation primary response protein 88 (MYD88) and Toll-like receptor (TLR) signalling⁸⁷. Monocyte emigration from the bone marrow is normal during early-stage infection of mice lacking either MYD88 or the type I interferon receptor (IFNAR); however, the combined loss of MYD88 and IFNAR results in a dramatic reduction in early monocyte recruitment⁸⁸ (FIG. 3). Thus, the contributions of these two signalling pathways to the regulation of monocyte emigration from the bone marrow provide redundancy to ensure innate immune antimicrobial defence. On the other hand, monocytopoiesis and the replenishment of bone marrow monocytes are reduced in MYD88-deficient mice after the third day of infection with L. monocytogenes⁸⁹.

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Figure 3

Pro-inflammatory cytokines and TLR ligands promote the emigration of LY6C^hi monocytes from the bone marrow

Monocyte emigration from the bone marrow during the early stage of infection with Listeria monocytogenes is normal in mice that lack either myeloid differentiation primary response protein 88 (MYD88; which is required for most responses to Toll-like receptor (TLR) ligands) or the type I interferon receptor (IFNAR). However, the combined loss of MYD88 and IFNAR impairs monocyte emigration from the bone marrow. IFN, interferon.

Recruitment of LY6C^hi monocytes into the spleen during L. monocytogenes infection is mediated in part by CX₃CR1 expression on monocytes and CX₃CL1 expression in the marginal zone and T cell areas of the spleen²⁷. Splenic DCs also coordinate monocyte and NK cell recruitment to the infected spleen by mechanisms that remain incompletely defined. Recruited NK cells, in proximity to recruited LY6C^hi monocytes, produce interferon-γ (IFNγ) and thus drive monocyte differentiation into TIP DCs at the site of bacterial infection⁹⁰.

M. tuberculosis is an inhaled, respiratory pathogen that infects macrophages and DCs⁹¹. Although CCR2-deficient mice respond normally to infection with a low-dose inoculum of M. tuberculosis⁹², CCR2-dependent monocyte recruitment protects mice following infection with a high-dose inoculum⁹³. Monocyte CCR2 deficiency markedly reduces the expansion of F4/80^lowCD11c⁺ cell populations in the lungs of infected mice, presumably because of diminished monocyte recruitment. T cell activation and recruitment in response to M. tuberculosis infection is defective in CCR2-deficient mice; however, loss of CCR2 expression on T cells does not interfere with the in vivo control of M. tuberculosis growth⁹⁴. These results suggest that recruited inflammatory monocytes promote the activation of M. tuberculosis-specific T cells and/or their recruitment to the lung. In addition, recruited monocytes, following activation and differentiation in the lung, may directly defend against mycobacterial infection. LY6C^hi monocytes are the major iNOS-producing cell population during pulmonary M. tuberculosis infection. LY6C^hi monocytes isolated from the bone marrow and adoptively transferred into M. tuberculosis-infected mice traffic to the lung and differentiate into CD11c^hi DCs that express MHC class II and high levels of iNOS⁹⁵. These cells are phenotypically similar to TIP DCs and require CCR2 to exit the bone marrow but not for trafficking into the infected lung.

Although monocytes provide protection against M. tuberculosis, a recent study using intranasal polyinosinic–polycytidylic acid (polyI:C) administration demonstrates that monocyte recruitment can also be deleterious⁹⁶. M. tuberculosis-infected mice treated with polyI:C succumbed to infection and harboured higher numbers of mycobacteria in the lungs. Defective mycobacterial clearance in polyI:C-treated mice was dependent on type I IFN-mediated induction of CCL2, which triggered enhanced recruitment of monocytes. In this setting, monocyte recruitment impaired microbial clearance, in part because monocytes harboured live M. tuberculosis. Thus, although CCR2-dependent recruitment of monocytes is important for controlling M. tuberculosis infection, excessive recruitment of monocytes following polyI:C administration worsens the infection. It is possible that inflammatory monocytes differentiate abnormally in the presence of high levels of type I IFNs, rendering them incapable of killing M. tuberculosis.

Protozoal infections

Toxoplasma gondii is a protozoal pathogen that infects mammalian cells and causes severe infections in immunocompromised hosts. Experimental studies have demonstrated that diminished recruitment of LY6C^hi monocytes renders CCR2− or CCL2-deficient mice more susceptible to T. gondii infection following peritoneal or oral inoculation^97,98. By contrast, in vivo depletion of neutrophils, which are also recruited to the intestine, does not worsen infection⁹⁹. LY6C^hi monocytes are recruited to the lamina propria of the intestine, where they differentiate into major producers of iNOS and TNF. In CCR2− or CCL2-deficient mice, TNF and iNOS production are markedly reduced in intestinal villi because LY6C^hi monocytes are retained in the bone marrow⁹⁸. Adoptive transfer of LY6C^hi monocytes to T. gondii-infected CCR2-deficient mice reduces mortality⁹⁸. This study also demonstrated that, once monocytes have exited the bone marrow, their trafficking to the intestinal lamina propria during T. gondii infection is CCR2 independent⁹⁸. It remains unclear how inflammatory monocytes are recruited to the lamina propria of the gut during T. gondii infection and how they mediate protection at this site. In contrast to the TIP DCs that are induced by L. monocytogenes infection⁸⁴, the TNF- and iNOS-producing cells that differentiate from LY6C^hi monocytes during T. gondii infection do not express CD11c, a result that may be attributable to distinct inflammatory responses to these two pathogens.

Inflammatory monocytes and DCs are recruited to dermal sites of infection and have been implicated in the induction of a T helper 1 (T_H1)-type response to L. major infection¹⁰⁰. In the skin, L. major multiplies within the vacuoles of phagocytic cells and induces an inflammatory response that includes the production of TNF and iNOS. A recent study demonstrated that TNF and iNOS are produced by CD11b⁺LY6C⁺CD11c⁺ DCs that are recruited to sites of infection in a CCR2-dependent manner¹⁰¹, although it is unclear whether CCR2 is involved in the step beyond bone marrow emigration. These recruited cells are probably derived from circulating LY6C^hi monocytes and harbour most of the L. major parasites in cutaneous lesions and draining lymph nodes.

During infection of mice with Plasmodium chabaudi, which serves as a model for malaria, LY6C^hi monocytes are recruited to the spleen, produce iNOS and phagocytose parasites. Adoptive transfer of LY6C^hi monocytes protects otherwise susceptible Ccr2^−/− mice during P. chabaudi infection, demonstrating the importance of monocytes for resistance to malaria infections¹⁰².

In contrast to the role of monocytes in L. major or P. chabaudi infections, CCR2-dependent recruitment of monocytes during Trypanosoma brucei infection is deleterious and enhances pathology. CCR2-deficient mice are more resistant to T. brucei infection and have decreased numbers of circulating parasites¹⁰³. T. brucei infection induces CCR2-dependent monocyte emigration from the bone marrow and CCR2-independent trafficking into the liver, where monocytes undergo IFNγ- and MYD88-dependent differentiation into TIP DCs. During T. brucei infection, IL-10 administration reduces monocyte emigration from the bone marrow, potentially by preventing the induction of CCL2 expression¹⁰⁴. In contrast to T. gondii, L. major and P. chabaudi, all of which are intracellular pathogens, T. brucei circulates in the plasma and remains extracellular during infection. Thus, differences in cellular localization may provide a partial explanation for the disparate effects of monocyte recruitment on host survival following infection with these pathogens.

Fungal infections

Yeasts and moulds are an important class of pathogens that cause infections in mammalian hosts. Cryptococcus neoformans is an environmental fungus that causes pulmonary infections on inhalation. Studies in CCR2-deficient mice have demonstrated that preventing the recruitment of monocytes alters the CD4⁺ T cell response from a predominantly T_H1-type response to a T_H2-type response, resulting in prolonged and more severe pulmonary infection and pathology¹⁰⁵. CCR2 deficiency also resulted in decreased accumulation of CD11b⁺ DCs in lung-draining lymph nodes¹⁰⁶ and decreased recruitment of LY6C^hi monocytes to the lung during C. neoformans infection¹⁰⁷. Thus, these studies suggest that LY6C^hi monocytes are recruited in a CCR2-dependent manner and contribute to the accumulation of CD11b⁺ DCs in lung-draining lymph nodes during fungal infection.

Aspergillus fumigatus is an inhaled fungal pathogen that can cause invasive infections in immunocompromised patients¹⁰⁸. A. fumigatus spores are common in the environment and are inhaled on a daily basis. In some individuals, A. fumigatus induces T_H2 cell responses that exacerbate asthma and lead to allergic bronchopulmonary aspergillosis. The role of inflammatory monocytes in defence against A. fumigatus was recently investigated using a CCR2 depleter mouse strain, in which the Ccr2 promoter drives expression of the simian diphtheria toxin receptor¹⁰⁹. In these mice, administration of diphtheria toxin rapidly but transiently depletes LY6C^hi monocytes from the bone marrow, bloodstream and peripheral tissues. Depletion of monocytes at the time of pulmonary infection with A. fumigatus markedly decreases the delivery of fungal spores to draining lymph nodes and prevents priming of A. fumigatus-specific CD4⁺ T cells. Thus, LY6C^hi monocytes — and potentially the CD11b⁺ pulmonary DCs that are derived from these cells — deliver spores from the lung to lymph nodes¹⁰⁹. Although CD11b⁺ DCs are capable of activating naive T cells, it remains unclear whether inflammatory monocytes delivering A. fumigatus spores to the lung directly prime T cells or whether they transfer antigens to classical DCs in the lymph nodes. Depletion of monocytes at later stages of A. fumigatus infection does not impair the activation or proliferation of responding CD4⁺ T cells, but does impair T_H1 cell development and promotes increased T_H17 cell differentiation in the lung¹¹⁰. Thus, recruitment of monocytes to the lung during fungal infection influences the differentiation of responding CD4⁺ T cells.

Blastomyces dermatitidis is a fungus that causes pulmonary infections following inhalation of spores. A recent study of the immune response to an attenuated B. dermatitidis vaccine demonstrated that LY6C^hi monocytes phagocytose this fungus at dermal sites of immunization and traffic to draining lymph nodes¹¹¹. Analyses of vaccine antigens associated with DCs in lymph nodes support a model of antigen transfer from LY6C^hi monocyte-derived DCs to classical DCs before T cell priming. Thus, in this model, monocyte-derived DCs do not directly induce naive T cells to upregulate activation markers or to proliferate, whereas classical DCs and migratory dermal DCs that express DEC205 potently activate naive antigen-specific T cells.

Viral infections

In mice infected with murine cytomegalovirus (MCMV), inflammatory monocytes are recruited to the liver and produce CCL3, which recruits NK cells¹¹². Monocyte recruitment to the liver is CCR2 dependent but, as in other infectious diseases, CCR2 signalling is only required for monocyte emigration from the bone marrow. Although CCL2 is produced by hepatocytes during MCMV infection¹¹³, the induction of type I IFNs also drives expression of CCL2, CCL12 and, to a lesser extent, CCL7 in bone marrow cells. Surprisingly, F4/80⁺ monocytes produce CCL2 in the bone marrow, leading to the suggestion that these cells might promote their own entry into the bloodstream¹¹⁴.

MCMV and vaccinia virus induce type I IFN expression by LY6C^hi monocytes¹¹⁵. This process requires TLR2 stimulation by viral ligands in endosomes of virusinfected cells. Following recruitment to sites of infection, inflammatory monocytes respond to distinct pathogen-derived ligands and differentiate along distinct pathways. In the case of MCMV and vaccinia virus infection, this response includes the induction of type I IFNs, in contrast to the response to bacterial and fungal infections.

LY6C^hi monocytes are recruited to the brain and enhance viral clearance in mice infected with West Nile virus (WNV)¹¹⁶. Adoptive transfer of wild-type or CCR2-deficient monocytes into WNV-infected recipients showed that CCR2 mediates monocyte emigration from the bone marrow but not entry into the brain. CCR2-deficient mice also show delayed viral clearance and impaired T cell responses during herpes simplex virus infections. In this model, adoptive transfer of wild-type or CCR2-deficient monocytes showed that trafficking of monocytes from the blood into infected vaginal tissues is dependent on CCR2. Although this finding suggests that CCR2 is required for monocyte infiltration of virus-infected vaginal tissues, the possibility that transferred CCR2-deficient monocytes returned to and became trapped in the bone marrow was not excluded.

Monocytes appear to have both beneficial and detrimental roles during influenza virus infections. CCR2-deficient mice show impaired monocyte recruitment to the lungs during infection with influenza virus, and this is associated with decreased immunopathology and mortality^117,118. On the other hand, CCR2-deficient mice also show delayed clearance of influenza virus as a result of diminished priming of virus-specific CD8⁺ T cells. Adoptive transfer of LY6C^hi monocytes into CCR2-deficient mice restores CD8⁺ T cell responses and promotes clearance of the virus. Interestingly, activation of the nuclear receptor peroxisome proliferator-activated receptor-γ (PPARγ) with the drug pioglitazone led to decreased CCL2 expression and reduced recruitment of LY6C^hi monocytes in vivo; these mice showed lower levels of mortality following influenza virus infection but were still able to effectively clear the infection¹¹⁸. This study suggests that monocyte recruitment can be pharmacologically manipulated during infection to minimize immunopathological tissue damage while maintaining microbial clearance.

Alveolar epithelial cells have been shown to produce CCL2 following infection with influenza virus in vitro, and this can facilitate monocyte trafficking across an epithelial cell monolayer¹¹⁹. However, the stage at which CCR2 and its chemokine ligands contribute to monocyte recruitment during respiratory viral infection in vivo remains unclear. It is possible that, in addition to mediating monocyte recruitment from the bone marrow to the bloodstream, CCR2 contributes to the direct recruitment of inflammatory monocytes from the bloodstream to the infected lungs, or from the bloodstream to lymph nodes that drain infected sites¹²⁰.

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Monocyte recruitment in sterile inflammation

Monocyte recruitment contributes to the pathogenesis of various aseptic inflammatory conditions. Studies in these disease models, particularly atherosclerosis^4,59, provide insights into the mechanisms of monocyte recruitment in aseptic, and often chronic, inflammatory settings.

Monocytes have long been associated with atherosclerosis^4,40. Monocytes are recruited to fatty streaks (the earliest visible atherosclerotic lesions, consisting of lipid deposits and infiltrating cells in the intima of arteries¹²¹), although the mechanisms of monocyte recruitment at this stage of disease remain undefined. During the progression of atherosclerosis, both LY6C^hi and LY6C^low monocytes are recruited to atherosclerotic plaques. Monocytes initially undergo P-selectin- and E-selectin-dependent rolling and then use VCAM1 for firm adhesion⁶¹. LY6C^hi monocytes are recruited to the lesions in a CCR2-, CCR5- and CX₃CR1-dependent manner^41,122,123, and they contribute to inflammation by differentiating into macrophages¹²⁴. Combined inhibition of CCL2, CCR5 and CX₃CR1 signalling abrogated monocyte recruitment and almost abolished atherosclerosis in Apoe^−/− mice, which are predisposed to the disease¹²⁵. LY6C^low monocytes infiltrate lesions to a lesser extent, and their recruitment has been less well characterized. They require CCR5, but not CX₃CR1, for migration to lesions⁴¹. Although LY6C^low monocytes exhibit phagocytic and pro-angiogenic functions, and might differentiate into DCs in the lesion, their role in disease progression remains largely unknown¹²⁶.

Myocardial infarction triggers an acute inflammatory response in patients, with an early peak of circulating inflammatory CD16⁻ monocytes followed by an increase in circulating CD16⁺ monocytes, supporting a model of biphasic monocyte recruitment to the heart¹²⁷. A similar temporal difference in the recruitment of LY6C^hi and LY6C^low monocytes has been demonstrated in a mouse model of myocardial infarction¹²⁸. These studies suggest that distinct subsets of monocytes are recruited at different stages of infarction, with the first wave of monocytes facilitating the removal of dead cardiac myocytes and the later phase promoting the resolution of inflammation and tissue repair¹²⁹. A recent study demonstrated that monocyte recruitment to the myocardium following infarction begins with their release from a reservoir in the spleen¹³⁰. In this setting, the vasoconstrictive hormone angiotensin II promotes the release of LY6C^hi monocytes from the spleen, independently of CCR2 signalling. Whether angiotensin II-mediated release of LY6C^hi monocytes from the spleen contributes to monocyte recruitment in other inflammatory settings is unclear.

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From the bone marrow to the bloodstream

As discussed above, one of the key roles of CCR2 is in mediating monocyte egress from the bone marrow into the circulation. It has been shown that the increase in circulating monocytes that is associated with infection or sterile inflammation (such as that induced by high-fat diets) is mediated by CCL2 and CCL7 triggering CCR2 signalling in LY6C^hi monocytes in the bone marrow¹⁸. However, how infections at peripheral sites can promote monocyte emigration from the bone marrow has remained a mystery.

Microbial infections induce granulocytosis, but the mechanisms that govern monocytosis and granulocytosis are distinct. Granulocytes are retained in the bone marrow by CXCL12-mediated CXCR4 signalling and exit in response to CXCR2 ligands, such as CXCL2 (also known as MIP2) and CXCL1 (also known as KC), which are produced during infection^131,132. Although CCL2 and CCL7 enhance CCR2-mediated monocyte emigration from the bone marrow, it is unclear whether a retention mechanism is in place to retain LY6C^hi monocytes in the bone marrow, as in the case of granulocytes. Inflammatory monocytes express CXCR4, but blocking CXCR4 under homeostatic conditions only minimally increases circulating monocyte frequencies (while markedly enhancing granulocyte frequencies)¹³³. On the other hand, blockade of both CXCR4 and CCR2 does increase circulating monocyte frequencies, suggesting that CXCR4 may promote the return of monocytes from the bloodstream to the bone marrow¹³³.

A recent study has provided new insights into the mechanisms controlling monocyte emigration from the bone marrow during infection¹³⁴. During L. monocytogenes infection in mice, it was shown that CCL2 production by bone marrow stromal cells, but not by haematopoietic cells, could promote monocyte emigration into the bloodstream¹³⁴. These bone marrow cells — which were identified as mesenchymal stem cells (MSCs)¹³⁵ and CXCL12-abundant reticular cells (CAR cells)¹³⁶ — express TLRs and respond to very low concentrations of TLR ligands by producing CCL2 (REF. 134). This provides a mechanism for peripheral infections to promote monocytosis (FIG. 4). Interestingly, in addition to TLR ligands, type I IFNs can promote the release of monocytes from the bone marrow during L. monocytogenes infection⁸⁸. Thus, it is possible that bone marrow stromal cells can respond to either circulating microbial molecules or pro-inflammatory cytokines induced by infection. These results suggest that monocyte release from the bone marrow is a crucial defence mechanism that can be readily triggered.

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Figure 4

Models of CCL2-mediated emigration of LY6C^hi monocytes from the bone marrow

Release of LY6C^hi monocytes from the bone marrow during Listeria monocytogenes infection is driven by CC-chemokine ligand 2 (CCL2). CCL2 is produced by mesenchymal stem cells (MSCs) and CXCL12-abundant reticular (CAR) cells following stimulation with circulating Toll-like receptor (TLR) ligands. Two models are proposed for the emigration of monocytes following CCL2 expression by these bone marrow stromal cells. In the first model, CCL2 production in the bone marrow increases monocyte chemokinesis, thereby increasing the likelihood that monocytes will come into contact with blood vessels. Subsequent movement of monocytes into the lumen may be independent of CC-chemokine receptor 2 (CCR2) (a). In the second model, CCL2 produced in proximity to vascular sinuses binds to glycosaminoglycans (GAGs) and forms a chemokine gradient that attracts monocytes to the abluminal aspect of the endothelium and then guides their transmigration into the circulation (b).

The cost of triggering monocyte release from the bone marrow is relatively low. The cells can enter the circulation and peruse the periphery, returning to the bone marrow in the absence of peripheral inflammation or entering sites of infection by adhering to the endothelial surface. How CCL2 production by local stromal cells induces monocytes to leave the bone marrow is unclear and requires further study. One possibility is that CCL2 production in proximity to vascular sinuses in the bone marrow attracts monocytes to the abluminal aspect of the endothelium and that subsequent movement to the lumen is automatic and independent of CCR2 or other chemokine-mediated signals. This scenario might explain the 80% reduction in homeostatic levels of monocyte emigration from the bone marrow that is seen in CCR2-deficient mice.

Such sensitive detection of circulating microbial molecules or pro-inflammatory cytokines by bone marrow stromal cells, with commensurate induction of CCL2, provides a mechanism to modulate the frequency of circulating inflammatory monocytes. Certain diseases, such as atherosclerosis, are associated with CCR2-mediated recruitment of inflammatory monocytes to the affected tissue site, and it is likely that circulating microbial molecules can, by modulating circulating monocyte frequencies, influence disease progression. It has been shown that bacterial cell wall fragments from the microbiota are present in the bloodstream and activate granulocytes, thereby enhancing their antimicrobial activity¹³⁷. It is likely that these and other microbial molecules, such as those absorbed from the gastrointestinal tract or other mucosal tissues, can also stimulate the release of monocytes from the bone marrow by inducing CCL2 production from MSCs and CAR cells. On the one hand, this would enhance antimicrobial defence during infection but, on the other hand, it would also exacerbate inflammatory processes at distinct tissue sites in the absence of infection. Notably, this suggests a mechanism whereby microorganisms may drive inflammatory disease in an otherwise ‘sterile’ tissue environment.

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Summary

Monocytes have an essential role in antimicrobial immune defence and promote tissue healing but, like a double-edged sword, they also contribute to tissue destruction during some infections and inflammatory diseases. Recent studies have furthered our understanding of monocyte heterogeneity and the mechanisms that mediate their trafficking. Infection with diverse pathogens (including bacteria, parasites, fungi and viruses) induces the recruitment of LY6C^hi monocytes to sites of infection, where they restrict further microbial growth and invasion. Emigration of LY6C^hi monocytes from the bone marrow is mediated by CCR2 and is modulated by bone marrow stromal cells, which sense circulating microbial molecules and produce CCL2. Although it has been shown that certain adhesion mechanisms target monocytes to sites of infection, much remains to be learnt about the trafficking of monocytes and the cues that direct them into different tissues once they emigrate from the bone marrow. Deciphering the process of monocyte recruitment may provide insights for the development of new therapeutics to manipulate the number and distribution of monocytes and to enhance antimicrobial immune defence or dampen detrimental inflammation.

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Acknowledgments

This work was supported by US National Institutes of Health grants 5R37AI039031 and 5P01CA023766-31 (to E.G.P.).

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Glossary

Polyinosinic–polycytidylic acid (polyI:C)	A substance that is used as a mimic of viral double-stranded RNA
CXCL12-abundant reticular cells (CAR cells)	A type of stromal cell found in the bone marrow that expresses high levels of CXC-chemokine ligand 12. CAR cells can function as adipo-osteogenic progenitors, which are required for the proliferation of haematopoietic stem cells (HSCs), B cells and erythroid progenitor cells, and for maintaining HSCs in an undifferentiated state

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Footnotes

Competing interests statement

The authors declare no competing financial interests.

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Monocyte recruitment during infection and inflammation.

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Abstract

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Monocyte recruitment during infection and inflammation

Abstract

Monocyte subsets and chemokine receptors

Table 1

Chemokines and monocyte trafficking

CCR2 and its ligands

The CX3CL1–CX3CR1 axis

CCR1 and CCR5

CCR6, CCR7, CCR8 and CXCR2

Adhesion molecules in monocyte trafficking

Table 2

Constitutive trafficking of monocytes

Monocyte recruitment to sites of infection

Box 1

Monocyte recruitment to lymph nodes during infection

Bacterial infections

Protozoal infections

Fungal infections

Viral infections

Monocyte recruitment in sterile inflammation

From the bone marrow to the bloodstream

Summary

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