Figure 4

Movies Reveal Transcriptional Bursting and State-Switching Dynamics in Individual Cells

(A) Distribution of Nanog and Oct4 production rates from representative movies in serum + LIF, and Gaussian fits to the components. Production rates were extracted from a total of 376 and 103 tracked cell cycles for Nanog and Oct4, respectively.

(B) Production rate distributions of individual cell lineage trees, each consisting of closely related cells descending from a single cell. Lineage trees are color-coded by the state they spend the majority of time in.

(C) Example single lineage traces exhibiting step-like changes in production rates within a state.

(D) Cell cycle phase distribution of steps within the Nanog-high state. Step occurrences are normalized by the frequencies of each cell cycle phase observed in the tracked data.

(E) Representative trace showing apparent steps from simulations under the bursty transcription model, using parameters estimated from mRNA distribution for the Nanog-high state (see Supplemental Information; see Figure S4E for simulation of Oct4 dynamics).

(F) Example traces of individual cells switching between Nanog-low and Nanog-high states.

(G) Empirical transition rates (mean ± SD) between the two Nanog states (N^Hi, Nanog-high; N^Lo, Nanog-low).