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FIG. 1.

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Zymogram assay to determine lytic activity of Tal2009 in gels containing 0.4% (wt/vol) L. lactis UC509.9 autoclaved cells. Each well was loaded with E. coli M15 lysates with proteins expressed from the following plasmids: lane 1, pQE60; lane 2, pTal2009-1; lane 3, pTal2009-2ΔN; lane 4, pTal2009-3ΔN; lane 5, pTal2009-4ΔN; lane 6, pTal2009-5ΔN; lane 7, pTal2009-6ΔN; lane 8, pTal2009ΔC. Molecular masses are indicated to the left of the gel, and a white arrow denotes the ~30-kDa processed proteins with lytic activity.

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