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Chromosomal context dependence of a eukaryotic recombinational hot spot.

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  • 1. Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
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    • Ponticelli AS 1
    (1 author)

Proceedings of the National Academy of Sciences of the United States of America, 01 Jan 1992, 89(1):227-231
https://doi.org/10.1073/pnas.89.1.227 PMID: 1729693 PMCID: PMC48209

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Abstract 

The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.

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Proc Natl Acad Sci U S A. 1992 Jan 1; 89(1): 227–231.
PMCID: PMC48209
PMID: 1729693

Chromosomal context dependence of a eukaryotic recombinational hot spot.

A S Ponticelli and G R Smith
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Abstract

The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
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NIGMS NIH HHS (1)